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1000 Titel
  • Monitoring the immune response to vaccination with an inactivated vaccine associated to bovine neonatal pancytopenia by deep sequencing transcriptome analysis in cattle
1000 Autor/in
  1. Demasius, Wiebke |
  2. Weikard, Rosemarie |
  3. Hadlich, Frieder |
  4. Müller, Kerstin Elisabeth |
  5. Kühn, Christa |
1000 Erscheinungsjahr 2013
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2013-10-07
1000 Erschienen in
1000 Quellenangabe
  • 44: 93
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2013
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1186/1297-9716-44-93 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3851820/ |
1000 Ergänzendes Material
  • https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-44-93#Declarations |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Bovine neonatal pancytopenia (BNP) is a new fatal, alloimmune/alloantibody mediated disease of new-born calves induced by ingestion of colostrum from cows, which had been vaccinated with a specific vaccine against the Bovine Virus Diarrhoea Virus (BVDV). The hypothesis of pathogenic MHC class I molecules in the vaccine had been put up, but no formal proof of specific causal MHC class I alleles has been provided yet. However, the unique features of the vaccine obviously result in extremely high specific antibody titres in the vaccinated animals, but apparently also in further molecules inducing BNP. Thus, a comprehensive picture of the immune response to the vaccine is essential. Applying the novel approach of next generation RNA sequencing (RNAseq), our study provides a new holistic, comprehensive analysis of the blood transcriptome regulation after vaccination with the specific BVDV vaccine. Our RNAseq approach identified a novel cytokine-like gene in the bovine genome that is highly upregulated after vaccination. This gene has never been described before in any other species and might be specific to ruminant immune response. Furthermore, our data revealed a very coordinated immune response to double-stranded (ds) RNA or a dsRNA analogue after vaccination with the inactivated single-stranded (ss) RNA vaccine. This would suggest either a substantial contamination of the vaccine with dsRNA from host cells after virus culture or a dsRNA analogue applied to the vaccine. The first option would highlight the potential risks associated with virus culture on homologous cells during vaccine production; the latter option would emphasise the potential risks associated with immune stimulating adjuvants used in vaccine production.
1000 Fachgruppe
  1. Biologie |
  2. Agrarwissenschaften |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/RGVtYXNpdXMsIFdpZWJrZQ==|https://frl.publisso.de/adhoc/creator/V2Vpa2FyZCwgUm9zZW1hcmll|https://frl.publisso.de/adhoc/creator/SGFkbGljaCwgRnJpZWRlcg==|https://frl.publisso.de/adhoc/creator/TcO8bGxlciwgS2Vyc3RpbiBFbGlzYWJldGg=|https://frl.publisso.de/adhoc/creator/S8O8aG4sIENocmlzdGE=
1000 Förderer
  1. German Ministry of Food, Agriculture, and Consumer Protection (BMELV) |
  2. Federal Office for Agriculture and Food (BLE) |
1000 Fördernummer
  1. -
  2. 2810HS27
1000 Förderprogramm
  1. -
  2. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Ministry of Food, Agriculture, and Consumer Protection (BMELV) |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Federal Office for Agriculture and Food (BLE) |
    1000 Förderprogramm -
    1000 Fördernummer 2810HS27
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405558.rdf
1000 Erstellt am 2017-11-29T16:13:11.468+0100
1000 Erstellt von 218
1000 beschreibt frl:6405558
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Tue May 29 13:46:42 CEST 2018
1000 Objekt bearb. Tue May 29 13:46:42 CEST 2018
1000 Vgl. frl:6405558
1000 Oai Id
  1. oai:frl.publisso.de:frl:6405558 |
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