WeightNameValue
1000 Titel
  • Pknox1/Prep1 Regulates Mitochondrial Oxidative Phosphorylation Components in Skeletal Muscle
1000 Autor/in
  1. Kanzleiter, Timo |
  2. Rath, Michaela |
  3. Penkov, Dmitry |
  4. Schulz, Nadja |
  5. Blasi, Francesco |
  6. Schürmann, Annette |
  7. Puchkov, Dmytro |
1000 Erscheinungsjahr 2013
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2013-11-11
1000 Erschienen in
1000 Quellenangabe
  • 34(2): 290-298
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3911294/ |
  • http://doi.org/10.1128/MCB.01232-13 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The homeodomain transcription factor Prep1 was previously shown to regulate insulin sensitivity. Our aim was to study the specific role of Prep1 for the regulation of energy metabolism in skeletal muscle. Muscle-specific ablation of Prep1 resulted in increased expression of respiratory chain subunits. This finding was consistent with an increase in mitochondrial enzyme activity without affecting mitochondrial volume fraction as assessed by electron microscopy. Metabolic phenotyping revealed no differences in daily energy expenditure or body composition. However, during treadmill exercise challenge, Prep1 ablation resulted in a higher maximal oxidative capacity and better endurance. Elevated PGC-1α expression was identified as a cause for increased mitochondrial capacity in Prep1 ablated mice. Prep1 stabilizes p160 Mybbp1a, a known inhibitor of PGC-1α activity. Thereby, p160 protein levels were significantly lower in the muscle of Prep1 ablated mice. By a chromatin immunoprecipitation-sequencing (ChIP-seq) approach, PREP1 binding sites in genes encoding mitochondrial components (e.g., Ndufs2) were identified that might be responsible for elevated proteins involved in oxidative phosphorylation (OXPHOS) in the muscle of Prep1 null mutants. These results suggest that Prep1 exhibits additional direct effects on regulation of mitochondrial proteins. We therefore conclude that Prep1 is a regulator of oxidative phosphorylation components via direct and indirect mechanisms.
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/S2FuemxlaXRlciwgVGltbw==|https://frl.publisso.de/adhoc/creator/UmF0aCwgTWljaGFlbGE=|https://frl.publisso.de/adhoc/creator/UGVua292LCBEbWl0cnk=|https://frl.publisso.de/adhoc/creator/U2NodWx6LCBOYWRqYQ==|https://frl.publisso.de/adhoc/creator/Qmxhc2ksIEZyYW5jZXNjbw==|https://frl.publisso.de/adhoc/creator/U2Now7xybWFubiwgQW5uZXR0ZQ==|http://orcid.org/0000-0001-8341-4847
1000 Förderer
  1. European Union |
  2. German Ministry of Education and Research |
  3. State of Brandenburg |
  4. AIRC (Italian Association for Cancer Research) |
1000 Fördernummer
  1. -
  2. 01GI0922
  3. -
  4. -
1000 Förderprogramm
  1. FP7 PREPROBEDIA
  2. -
  3. -
  4. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer European Union |
    1000 Förderprogramm FP7 PREPROBEDIA
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer German Ministry of Education and Research |
    1000 Förderprogramm -
    1000 Fördernummer 01GI0922
  3. 1000 joinedFunding-child
    1000 Förderer State of Brandenburg |
    1000 Förderprogramm -
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer AIRC (Italian Association for Cancer Research) |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405598.rdf
1000 Erstellt am 2017-11-30T14:00:09.607+0100
1000 Erstellt von 25
1000 beschreibt frl:6405598
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Wed Aug 01 12:41:06 CEST 2018
1000 Objekt bearb. Wed Aug 01 12:41:06 CEST 2018
1000 Vgl. frl:6405598
1000 Oai Id
  1. oai:frl.publisso.de:frl:6405598 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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