WeightNameValue
1000 Titel
  • A Conformational Intermediate in Glutamate Receptor Activation
1000 Autor/in
  1. Lau, Albert Y. |
  2. Salazar, Héctor |
  3. Blachowicz, Lydia |
  4. Ghisi, Valentina |
  5. Roux, Benoît |
  6. Plested, Andrew |
1000 Erscheinungsjahr 2013
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2013-08-07
1000 Erschienen in
1000 Quellenangabe
  • 79(3): 492-503
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814226/ |
  • https://doi.org/10.1016/j.neuron.2013.06.003 |
1000 Ergänzendes Material
  • http://www.sciencedirect.com/science/article/pii/S0896627313004947?via%3Dihub#app3 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Ionotropic glutamate receptors (iGluRs) transduce the chemical signal of neurotransmitter release into membrane depolarization at excitatory synapses in the brain. The opening of the transmembrane ion channel of these ligand-gated receptors is driven by conformational transitions that are induced by the association of glutamate molecules to the ligand-binding domains (LBDs). Here, we describe the crystal structure of a GluA2 LBD tetramer in a configuration that involves an ∼30° rotation of the LBD dimers relative to the crystal structure of the full-length receptor. The configuration is stabilized by an engineered disulfide crosslink. Biochemical and electrophysiological studies on full-length receptors incorporating either this crosslink or an engineered metal bridge show that this LBD configuration corresponds to an intermediate state of receptor activation. GluA2 activation therefore involves a combination of both intra-LBD (cleft closure) and inter-LBD dimer conformational transitions. Overall, these results provide a comprehensive structural characterization of an iGluR intermediate state.
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TGF1LCBBbGJlcnQgWS4=|https://frl.publisso.de/adhoc/creator/U2FsYXphciwgSMOpY3Rvcg==|https://frl.publisso.de/adhoc/creator/QmxhY2hvd2ljeiwgTHlkaWE=|https://frl.publisso.de/adhoc/creator/R2hpc2ksIFZhbGVudGluYQ==|https://frl.publisso.de/adhoc/creator/Um91eCwgQmVub8OudA==|http://orcid.org/0000-0001-6062-0832
1000 Förderer
  1. NIH |
  2. DFG |
  3. HFSP |
1000 Fördernummer
  1. GM094495; GM062342
  2. EXC 257 (NeuroCure); Pl-619
  3. -
1000 Förderprogramm
  1. -
  2. -
  3. Long-Term Fellowship
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer NIH |
    1000 Förderprogramm -
    1000 Fördernummer GM094495; GM062342
  2. 1000 joinedFunding-child
    1000 Förderer DFG |
    1000 Förderprogramm -
    1000 Fördernummer EXC 257 (NeuroCure); Pl-619
  3. 1000 joinedFunding-child
    1000 Förderer HFSP |
    1000 Förderprogramm Long-Term Fellowship
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6405604.rdf
1000 Erstellt am 2017-12-01T10:37:16.367+0100
1000 Erstellt von 25
1000 beschreibt frl:6405604
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Wed Apr 11 10:44:10 CEST 2018
1000 Objekt bearb. Wed Apr 11 10:44:09 CEST 2018
1000 Vgl. frl:6405604
1000 Oai Id
  1. oai:frl.publisso.de:frl:6405604 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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