WeightNameValue
1000 Titel
  • Molecular basis for SH3 domain regulation of F-BAR–mediated membrane deformation
1000 Autor/in
  1. Haucke, Volker |
  2. Rao, Yijian |
  3. Ma, Qingjun |
  4. Vahedi-Faridi, Ardeschir |
  5. Sundborger, Anna |
  6. Pechstein, Arndt |
  7. Puchkov, Dmytro |
  8. Luo, Lin |
  9. Shupliakov, Oleg |
  10. Saenger, Wolfram |
1000 Erscheinungsjahr 2010
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2010-04-19
1000 Erschienen in
1000 Quellenangabe
  • 107(18): 8213–8218
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2889545/ |
  • http://doi.org/10.1073/pnas.1003478107 |
1000 Ergänzendes Material
  • http://www.pnas.org/cgi/content/full/1003478107/DCSupplemental |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Members of the Bin/amphiphysin/Rvs (BAR) domain protein superfamily are involved in membrane remodeling in various cellular pathways ranging from endocytic vesicle and T-tubule formation to cell migration and neuromorphogenesis. Membrane curvature induction and stabilization are encoded within the BAR or Fer-CIP4 homology-BAR (F-BAR) domains, α-helical coiled coils that dimerize into membrane-binding modules. BAR/F-BAR domain proteins often contain an SH3 domain, which recruits binding partners such as the oligomeric membrane-fissioning GTPase dynamin. How precisely BAR/F-BAR domain-mediated membrane deformation is regulated at the cellular level is unknown. Here we present the crystal structures of full-length syndapin 1 and its F-BAR domain. Our data show that syndapin 1 F-BAR-mediated membrane deformation is subject to autoinhibition by its SH3 domain. Release from the clamped conformation is driven by association of syndapin 1 SH3 with the proline-rich domain of dynamin 1, thereby unlocking its potent membrane-bending activity. We hypothesize that this mechanism might be commonly used to regulate BAR/F-BAR domain-induced membrane deformation and to potentially couple this process to dynamin-mediated fission. Our data thus suggest a structure-based model for SH3-mediated regulation of BAR/F-BAR domain function.
1000 Sacherschließung
lokal membrane bending
lokal endocytosis
lokal syndapin
lokal dynamin
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. http://orcid.org/0000-0003-3119-6993|https://frl.publisso.de/adhoc/creator/UmFvLCBZaWppYW4=|https://frl.publisso.de/adhoc/creator/TWEsIFFpbmdqdW4=|https://frl.publisso.de/adhoc/creator/VmFoZWRpLUZhcmlkaSwgQXJkZXNjaGly|https://frl.publisso.de/adhoc/creator/U3VuZGJvcmdlciwgQW5uYQ==|https://frl.publisso.de/adhoc/creator/UGVjaHN0ZWluLCBBcm5kdA==|https://frl.publisso.de/adhoc/creator/UHVjaGtvdiwgRG15dHJv|https://frl.publisso.de/adhoc/creator/THVvLCBMaW4=|https://frl.publisso.de/adhoc/creator/U2h1cGxpYWtvdiwgT2xlZw==|https://frl.publisso.de/adhoc/creator/U2FlbmdlciwgV29sZnJhbQ==
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
  2. Swedish Research Council |
  3. Wallenbergs Foundation |
  4. Linné Center |
  5. Excellence Cluster |
  6. European Science Foundation |
1000 Fördernummer
  1. -
  2. 13473; 20587
  3. -
  4. -
  5. -
  6. -
1000 Förderprogramm
  1. Sonderforschungsbereich (SFB) 449/A11; Forschergruppe (FOR) 806; SFB 449/Z3
  2. -
  3. -
  4. -
  5. 257-Neurocure
  6. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm Sonderforschungsbereich (SFB) 449/A11; Forschergruppe (FOR) 806; SFB 449/Z3
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Swedish Research Council |
    1000 Förderprogramm -
    1000 Fördernummer 13473; 20587
  3. 1000 joinedFunding-child
    1000 Förderer Wallenbergs Foundation |
    1000 Förderprogramm -
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer Linné Center |
    1000 Förderprogramm -
    1000 Fördernummer -
  5. 1000 joinedFunding-child
    1000 Förderer Excellence Cluster |
    1000 Förderprogramm 257-Neurocure
    1000 Fördernummer -
  6. 1000 joinedFunding-child
    1000 Förderer European Science Foundation |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406028.rdf
1000 Erstellt am 2017-12-28T11:06:11.052+0100
1000 Erstellt von 25
1000 beschreibt frl:6406028
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Thu Jan 30 23:38:38 CET 2020
1000 Objekt bearb. Tue May 29 07:15:53 CEST 2018
1000 Vgl. frl:6406028
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406028 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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