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1000 Titel
  • Identification of expression QTL (eQTL) of genes expressed in porcine M. longissimus dorsi and associated with meat quality traits
1000 Autor/in
  1. Ponsuksili, Siriluck |
  2. Murani, Eduard |
  3. Schwerin, Manfred |
  4. Schellander, Karl |
  5. Wimmers, Klaus |
1000 Erscheinungsjahr 2010
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2010-10-16
1000 Erschienen in
1000 Quellenangabe
  • 11: 572
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2010
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1186/1471-2164-11-572 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3091721/ |
1000 Ergänzendes Material
  • https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-11-572#Declarations |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Genetic analysis of transcriptional profiles is a promising approach for identifying and dissecting the genetics of complex traits like meat performance. Accordingly, expression levels obtained by microarray analysis were taken as phenotypes in a linkage analysis to map eQTL. Moreover, expression levels were correlated with traits related to meat quality and principle components with high loadings of these traits. By using an up-to-date annotation and localization of the respective probe-sets, the integration of eQTL mapping data and information of trait correlated expression finally served to point to candidate genes for meat quality traits. RESULTS: Genome-wide transcriptional profiles of M. longissimus dorsi RNAs samples of 74 F2 animals of a pig resource population revealed 11,457 probe-sets representing genes expressed in the muscle. Linkage analysis of expression levels of these probe-sets provided 9,180 eQTL at the suggestive significance threshold of LOD > 2. We mapped 653 eQTL on the same chromosome as the corresponding gene and these were designated as 'putative cis- eQTL'. In order to link eQTL to the traits of interest, probe-sets were addressed with relative transcript abundances that showed correlation with meat quality traits at p ≤ 0.05. Out of the 653 'putative cis- eQTL', 262 transcripts were correlated with at least one meat quality trait. Furthermore, association of expression levels with composite traits with high loadings for meat quality traits generated by principle component analysis were taken into account leading to a list of 85 genes exhibiting cis- eQTL and trait dependent expression. CONCLUSION: Holistic expression profiling was integrated with QTL analysis for meat quality traits. Correlations between transcript abundance and meat quality traits, combined with genetic positional information of eQTL allowed us to prioritise candidate genes for further study.
1000 Fachgruppe
  1. Biologie |
  2. Agrarwissenschaften |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/UG9uc3Vrc2lsaSwgU2lyaWx1Y2s=|https://frl.publisso.de/adhoc/creator/TXVyYW5pLCBFZHVhcmQ=|https://frl.publisso.de/adhoc/creator/U2Nod2VyaW4sIE1hbmZyZWQ=|https://frl.publisso.de/adhoc/creator/U2NoZWxsYW5kZXIsIEthcmw=|http://orcid.org/0000-0002-9523-6790
1000 Förderer
  1. German Research Foundation (DFG) |
1000 Fördernummer
  1. FOR 753
1000 Förderprogramm
  1. Forschergruppe 'DRIP'
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Research Foundation (DFG) |
    1000 Förderprogramm Forschergruppe 'DRIP'
    1000 Fördernummer FOR 753
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406087.rdf
1000 Erstellt am 2018-01-02T18:02:07.636+0100
1000 Erstellt von 218
1000 beschreibt frl:6406087
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Mon May 28 09:35:40 CEST 2018
1000 Objekt bearb. Mon May 28 09:35:40 CEST 2018
1000 Vgl. frl:6406087
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406087 |
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