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1000 Titel
  • Assessment of the immune capacity of mammary epithelial cells: comparison with mammary tissue after challenge with Escherichia coli
1000 Autor/in
  1. Günther, Juliane |
  2. Koczan, Dirk |
  3. Yang, Wei |
  4. Nürnberg, Gerd |
  5. Repsilber, Dirk |
  6. Schuberth, Hans-Joachim |
  7. Park, Zaneta |
  8. Maqbool, Nauman |
  9. Molenaar, Adrian |
  10. Seyfert, Hans-Martin |
1000 Erscheinungsjahr 2009
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2009-03-27
1000 Erschienen in
1000 Quellenangabe
  • 40(4): 31
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2009
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1051/vetres/2009014 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2695127/ |
1000 Ergänzendes Material
  • https://www.vetres.org/articles/vetres/full_html/2009/04/v08328/v08328.html#supplementary-material |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • We examined the repertoire and extent of inflammation dependent gene regulation in a bovine mammary epithelial cell (MEC) model, to better understand the contribution of the MEC in the immune defence of the udder. We challenged primary cultures of MEC from cows with heat inactivated Escherichia coli pathogens and used Affymetrix DNA-microarrays to profile challenge related alterations in their transcriptome. Compared to acute mastitis, the most prominently activated genes comprise those encoding chemokines, interleukins, beta-defensins, serum amyloid A and haptoglobin. Hence, the MEC exert sentinel as well as effector functions of innate immune defence. E. coli stimulated a larger fraction of genes (30%) in the MEC belonging to the functional category Inflammatory Response than we recorded with the same microarrays during acute mastitis in the udder (17%). This observation underscores the exquisite immune capacity of MEC. To more closely examine the adequacy of immunological regulation in MEC, we compared the inflammation dependent regulation of factors contributing to the complement system between the udder versus the MEC. In the MEC we observed only up regulation of several complement factor-encoding genes. Mastitis, in contrast, in the udder strongly down regulates such genes encoding factors contributing to both, the classical pathway of complement activation and the Membrane Attack Complex, while the expression of factors contributing to the alternative pathway may be enhanced. This functionally polarized regulation of the complex complement pathway is not reflected in the MEC models.
1000 Sacherschließung
lokal mammary epithelial cell
lokal mastitis
lokal transcriptome profiling
lokal immune capacity
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/R8O8bnRoZXIsIEp1bGlhbmU=|https://frl.publisso.de/adhoc/creator/S29jemFuLCBEaXJr|https://frl.publisso.de/adhoc/creator/WWFuZywgV2Vp|https://frl.publisso.de/adhoc/creator/TsO8cm5iZXJnLCBHZXJk|https://frl.publisso.de/adhoc/creator/UmVwc2lsYmVyLCBEaXJr|https://frl.publisso.de/adhoc/creator/U2NodWJlcnRoLCBIYW5zLUpvYWNoaW0=|https://frl.publisso.de/adhoc/creator/UGFyaywgWmFuZXRh|https://frl.publisso.de/adhoc/creator/TWFxYm9vbCwgTmF1bWFu|https://frl.publisso.de/adhoc/creator/TW9sZW5hYXIsIEFkcmlhbg==|https://frl.publisso.de/adhoc/creator/U2V5ZmVydCwgSGFucy1NYXJ0aW4=
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft (DFG) |
  2. European Community |
  3. Ministry of Research, Science and Technology (MORST), New Zealand |
1000 Fördernummer
  1. Se 326/14-2
  2. -
  3. -
1000 Förderprogramm
  1. Researcher Group FOR585
  2. EADGENE network-of-excellence
  3. Julius-von-Haast award; AgResearch
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm Researcher Group FOR585
    1000 Fördernummer Se 326/14-2
  2. 1000 joinedFunding-child
    1000 Förderer European Community |
    1000 Förderprogramm EADGENE network-of-excellence
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Ministry of Research, Science and Technology (MORST), New Zealand |
    1000 Förderprogramm Julius-von-Haast award; AgResearch
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406369.rdf
1000 Erstellt am 2018-01-18T15:36:30.751+0100
1000 Erstellt von 218
1000 beschreibt frl:6406369
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Thu Jan 30 23:49:11 CET 2020
1000 Objekt bearb. Wed May 02 12:38:22 CEST 2018
1000 Vgl. frl:6406369
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406369 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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