WeightNameValue
1000 Titel
  • SNARE motif-mediated sorting of synaptobrevin by the endocytic adaptors clathrin assembly lymphoid myeloid leukemia (CALM) and AP180 at synapses
1000 Autor/in
  1. Koo, Seong Joo |
  2. Markovic, Stefan |
  3. Puchkov, Dmytro |
  4. Mahrenholz, Carsten C. |
  5. Beceren-Braun, Figen |
  6. Maritzen, Tanja |
  7. Dernedde, Jens |
  8. Volkmer, Rudolf |
  9. Oschkinat, Hartmut |
  10. Haucke, Volker |
1000 Erscheinungsjahr 2011
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2011-08-16
1000 Erschienen in
1000 Quellenangabe
  • 108(33): 13540-13545
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158172/ |
  • https://doi.org/10.1073/pnas.1107067108 |
1000 Ergänzendes Material
  • http://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1107067108/-/DCSupplemental |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Neurotransmission depends on the exo-endocytosis of synaptic vesicles at active zones. Synaptobrevin 2 [also known as vesicle-associated membrane protein 2 (VAMP2)], the most abundant synaptic vesicle protein and a major soluble NSF attachment protein receptor (SNARE) component, is required for fast calcium-triggered synaptic vesicle fusion. In contrast to the extensive knowledge about the mechanism of SNARE-mediated exocytosis, little is known about the endocytic sorting of synaptobrevin 2. Here we show that synaptobrevin 2 sorting involves determinants within its SNARE motif that are recognized by the ANTH domains of the endocytic adaptors AP180 and clathrin assembly lymphoid myeloid leukemia (CALM). Depletion of CALM or AP180 causes selective surface accumulation of synaptobrevin 2 but not vGLUT1 at the neuronal surface. Endocytic sorting of synaptobrevin 2 is mediated by direct interaction of the ANTH domain of the related endocytic adaptors CALM and AP180 with the N-terminal half of the SNARE motif centered around M46, as evidenced by NMR spectroscopy analysis and site-directed mutagenesis. Our data unravel a unique mechanism of SNARE motif-dependent endocytic sorting and identify the ANTH domain proteins AP180 and CALM as cargo-specific adaptors for synaptobrevin endocytosis. Defective SNARE endocytosis may also underlie the association of CALM and AP180 with neurodevelopmental and cognitive defects or neurodegenerative disorders.
1000 Sacherschließung
lokal structure
lokal clathrin-mediated endocytosis
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/S29vLCBTZW9uZyBKb28=|https://frl.publisso.de/adhoc/creator/TWFya292aWMsIFN0ZWZhbg==|https://frl.publisso.de/adhoc/creator/UHVjaGtvdiwgRG15dHJv|https://frl.publisso.de/adhoc/creator/TWFocmVuaG9seiwgQ2Fyc3RlbiBDLg==|https://frl.publisso.de/adhoc/creator/QmVjZXJlbi1CcmF1biwgRmlnZW4=|https://frl.publisso.de/adhoc/creator/TWFyaXR6ZW4sIFRhbmph|https://frl.publisso.de/adhoc/creator/RGVybmVkZGUsIEplbnM=|https://frl.publisso.de/adhoc/creator/Vm9sa21lciwgUnVkb2xm|https://frl.publisso.de/adhoc/creator/T3NjaGtpbmF0LCBIYXJ0bXV0|http://orcid.org/0000-0003-3119-6993
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft (DFG) |
  2. European Science Foundation |
  3. Max Delbrück Center for Molecular Medicine |
1000 Fördernummer
  1. SFB958/A01; FOR806
  2. -
  3. -
1000 Förderprogramm
  1. Exc 257-Neurocure
  2. -
  3. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm Exc 257-Neurocure
    1000 Fördernummer SFB958/A01; FOR806
  2. 1000 joinedFunding-child
    1000 Förderer European Science Foundation |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Max Delbrück Center for Molecular Medicine |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406562.rdf
1000 Erstellt am 2018-01-30T15:05:15.509+0100
1000 Erstellt von 218
1000 beschreibt frl:6406562
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Fri Jan 31 02:55:05 CET 2020
1000 Objekt bearb. Wed Jun 20 12:40:38 CEST 2018
1000 Vgl. frl:6406562
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406562 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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