WeightNameValue
1000 Titel
  • Elucidating the principles of the molecular organization of heteropolymeric tight junction strands
1000 Autor/in
  1. Piontek, Jörg |
  2. Fritzsche, Susanne |
  3. Cording, Jimmi |
  4. Richter, Sandra |
  5. Hartwig, Jens |
  6. Walter, Maria |
  7. Yu, Dan |
  8. Turner, Jerrold R. |
  9. Gehring, Claudia |
  10. Rahn, Hans-Peter |
  11. Wolburg, Hartwig |
  12. Blasig, Ingolf E. |
1000 Erscheinungsjahr 2011
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2011-05-01
1000 Erschienen in
1000 Quellenangabe
  • 68(23): 3903–3918
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4336547/ |
  • https://doi.org/10.1007/s00018-011-0680-z |
1000 Ergänzendes Material
  • https://link.springer.com/article/10.1007%2Fs00018-011-0680-z#SupplementaryMaterial |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Paracellular barrier properties of tissues are mainly determined by the composition of claudin heteropolymers. To analyze the molecular organization of tight junctions (TJ), we investigated the ability of claudins (Cld) to form homo- and heteromers. Cld1, -2, -3, -5, and -12 expressed in cerebral barriers were investigated. TJ-strands were reconstituted by claudin-transfection of HEK293-cells. cis-Interactions and/or spatial proximity were analyzed by fluorescence resonance energy transfer inside and outside of strands and ranked: Cld5/Cld5 > Cld5/Cld1 > Cld3/Cld1 > Cld3/Cld3 > Cld3/Cld5, no Cld3/Cld2. Classic Cld1, -3, and -5 but not non-classic Cld12 showed homophilic trans-interaction. Freeze-fracture electron microscopy revealed that, in contrast to classic claudins, YFP-tagged Cld12 does not form homopolymers. Heterophilic trans-interactions were analyzed in cocultures of differently monotransfected cells. trans-Interaction of Cld3/Cld5 was less pronounced than that of Cld3/Cld1, Cld5/Cld1, Cld5/Cld5 or Cld3/Cld3. The barrier function of reconstituted TJ-strands was demonstrated by a novel imaging assay. A model of the molecular organization of TJ was generated.
1000 Sacherschließung
lokal Claudins
lokal Tight junction
lokal FRET
lokal Protein–protein interaction
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/UGlvbnRlaywgSsO2cmc=|https://frl.publisso.de/adhoc/creator/RnJpdHpzY2hlLCBTdXNhbm5l|https://frl.publisso.de/adhoc/creator/Q29yZGluZywgSmltbWk=|https://frl.publisso.de/adhoc/creator/UmljaHRlciwgU2FuZHJh|https://frl.publisso.de/adhoc/creator/SGFydHdpZywgSmVucw==|https://frl.publisso.de/adhoc/creator/V2FsdGVyLCBNYXJpYQ==|https://frl.publisso.de/adhoc/creator/WXUsIERhbg==|https://frl.publisso.de/adhoc/creator/VHVybmVyLCBKZXJyb2xkIFIu|https://frl.publisso.de/adhoc/creator/R2VocmluZywgQ2xhdWRpYQ==|https://frl.publisso.de/adhoc/creator/UmFobiwgSGFucy1QZXRlcg==|https://frl.publisso.de/adhoc/creator/V29sYnVyZywgSGFydHdpZw==|https://frl.publisso.de/adhoc/creator/Qmxhc2lnLCBJbmdvbGYgRS4=
1000 Förderer
  1. Deutsche Forschungsgemeinschaft (DFG) |
  2. National Institutes of Health (NIH) |
1000 Fördernummer
  1. BL308/7-3; BL308/7-4; PI 837/2-1
  2. DK61931
1000 Förderprogramm
  1. -
  2. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft (DFG) |
    1000 Förderprogramm -
    1000 Fördernummer BL308/7-3; BL308/7-4; PI 837/2-1
  2. 1000 joinedFunding-child
    1000 Förderer National Institutes of Health (NIH) |
    1000 Förderprogramm -
    1000 Fördernummer DK61931
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406565.rdf
1000 Erstellt am 2018-01-30T15:32:48.707+0100
1000 Erstellt von 218
1000 beschreibt frl:6406565
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Tue Jul 31 11:44:41 CEST 2018
1000 Objekt bearb. Tue Jul 31 11:44:40 CEST 2018
1000 Vgl. frl:6406565
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406565 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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