WeightNameValue
1000 Titel
  • Nucleocytoplasmic shuttling by nucleoporins Nup153 and Nup214 and CRM1-dependent nuclear export control the subcellular distribution of latent Stat1
1000 Autor/in
  1. Marg, Andreas |
  2. Shan, Ying |
  3. Meyer, Thomas |
  4. Meissner, Torsten |
  5. Brandenburg, Martin |
  6. Vinkemeier, Uwe |
1000 Erscheinungsjahr 2004
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2004-06-21
1000 Erschienen in
1000 Quellenangabe
  • 165(6): 823–833
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2172394/ |
  • https://doi.org/10.1083/jcb.200403057 |
1000 Ergänzendes Material
  • http://jcb.rupress.org/content/suppl/2004/06/15/jcb.200403057.DC1 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Interferon stimulation of cells leads to the tyrosine phosphorylation of latent Stat1 and subsequent transient accumulation in the nucleus that requires canonical transport factors. However, the mechanisms that control the predominantly cytoplasmic localization in unstimulated cells have not been resolved. We uncovered that constitutive energy- and transport factor-independent nucleocytoplasmic shuttling is a property of unphosphorylated Stat1, Stat3, and Stat5. The NH2- and COOH-terminal Stat domains are generally dispensable, whereas alkylation of a single cysteine residue blocked cytokine-independent nuclear translocation and thus implicated the linker domain into the cycling of Stat1. It is revealed that constitutive nucleocytoplasmic shuttling of Stat1 is mediated by direct interactions with the FG repeat regions of nucleoporin 153 and nucleoporin 214 of the nuclear pore. Concurrent active nuclear export by CRM1 created a nucleocytoplasmic Stat1 concentration gradient that is significantly reduced by the blocking of energy-requiring translocation mechanisms or the specific inactivation of CRM1. Thus, we propose that two independent translocation pathways cooperate to determine the steady-state distribution of Stat1.
1000 Sacherschließung
lokal transcription factor
lokal nuclear transport
lokal Stat1
lokal interferon
lokal nucleoporins
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TWFyZywgQW5kcmVhcw==|https://frl.publisso.de/adhoc/creator/U2hhbiwgWWluZw==|https://frl.publisso.de/adhoc/creator/TWV5ZXIsIFRob21hcw==|https://frl.publisso.de/adhoc/creator/TWVpc3NuZXIsIFRvcnN0ZW4=|https://frl.publisso.de/adhoc/creator/QnJhbmRlbmJ1cmcsIE1hcnRpbg==|https://frl.publisso.de/adhoc/creator/Vmlua2VtZWllciwgVXdl
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
  2. Bundesministerium für Bildung und Forschung |
  3. EMBO |
  4. Boehringer Ingelheim Fonds |
1000 Fördernummer
  1. -
  2. -
  3. -
  4. -
1000 Förderprogramm
  1. -
  2. Bio-Future
  3. Young-Investigator Program
  4. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Bundesministerium für Bildung und Forschung |
    1000 Förderprogramm Bio-Future
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer EMBO |
    1000 Förderprogramm Young-Investigator Program
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer Boehringer Ingelheim Fonds |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406584.rdf
1000 Erstellt am 2018-01-31T14:32:46.561+0100
1000 Erstellt von 22
1000 beschreibt frl:6406584
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Tue Jul 31 13:04:38 CEST 2018
1000 Objekt bearb. Tue Jul 31 13:04:37 CEST 2018
1000 Vgl. frl:6406584
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406584 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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