WeightNameValue
1000 Titel
  • Water Permeability of Asymmetric Planar Lipid Bilayers: Leaflets of Different Composition Offer Independent and Additive Resistances to Permeation
1000 Autor/in
  1. Krylov, Andrey V. |
  2. Pohl, Peter |
  3. Zeidel, Mark L. |
  4. Hill, Warren G. |
1000 Erscheinungsjahr 2001
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2001-10-01
1000 Erschienen in
1000 Quellenangabe
  • 118(4): 333–340
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2233699/ |
  • https://doi.org/10.1085/jgp.118.4.333 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • To understand how plasma membranes may limit water flux, we have modeled the apical membrane of MDCK type 1 cells. Previous experiments demonstrated that liposomes designed to mimic the inner and outer leaflet of this membrane exhibited 18-fold lower water permeation for outer leaflet lipids than inner leaflet lipids (Hill, W.G., and M.L. Zeidel. 2000. J. Biol. Chem. 275:30176–30185), confirming that the outer leaflet is the primary barrier to permeation. If leaflets in a bilayer resist permeation independently, the following equation estimates single leaflet permeabilities: 1/PAB = 1/PA + 1/PB (Eq. l), where PAB is the permeability of a bilayer composed of leaflets A and B, PA is the permeability of leaflet A, and PB is the permeability of leaflet B. Using for the MDCK leaflet–specific liposomes gives an estimated value for the osmotic water permeability (Pf) of 4.6 × 10−4 cm/s (at 25°C) that correlated well with experimentally measured values in intact cells. We have now constructed both symmetric and asymmetric planar lipid bilayers that model the MDCK apical membrane. Water permeability across these bilayers was monitored in the immediate membrane vicinity using a Na+-sensitive scanning microelectrode and an osmotic gradient induced by addition of urea. The near-membrane concentration distribution of solute was used to calculate the velocity of water flow (Pohl, P., S.M. Saparov, and Y.N. Antonenko. 1997. Biophys. J. 72:1711–1718). At 36°C, Pf was 3.44 ± 0.35 × 10−3 cm/s for symmetrical inner leaflet membranes and 3.40 ± 0.34 × 10−4 cm/s for symmetrical exofacial membranes. From , the estimated permeability of an asymmetric membrane is 6.2 × 10−4 cm/s. Water permeability measured for the asymmetric planar bilayer was 6.7 ± 0.7 × 10−4 cm/s, which is within 10% of the calculated value. Direct experimental measurement of Pf for an asymmetric planar membrane confirms that leaflets in a bilayer offer independent and additive resistances to water permeation and validates the use of.
1000 Sacherschließung
lokal apical membrane
lokal fluidity
lokal mock cells
lokal cell membrane permeability
lokal barrier function
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/S3J5bG92LCBBbmRyZXkgVi4=|http://orcid.org/0000-0002-1792-2314|https://frl.publisso.de/adhoc/creator/WmVpZGVsLCBNYXJrIEwu|https://frl.publisso.de/adhoc/creator/SGlsbCwgV2FycmVuIEcu
1000 Förderer
  1. National Kidney Foundation |
  2. National Institutes of Health |
  3. Deutsche Forschungsgemeinschaft |
1000 Fördernummer
  1. -
  2. DK43955
  3. Po 533/2-3; Po 533/7-1
1000 Förderprogramm
  1. -
  2. -
  3. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer National Kidney Foundation |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer National Institutes of Health |
    1000 Förderprogramm -
    1000 Fördernummer DK43955
  3. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer Po 533/2-3; Po 533/7-1
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406587.rdf
1000 Erstellt am 2018-01-31T15:12:07.781+0100
1000 Erstellt von 22
1000 beschreibt frl:6406587
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Thu Nov 22 13:00:58 CET 2018
1000 Objekt bearb. Thu Nov 22 13:00:57 CET 2018
1000 Vgl. frl:6406587
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406587 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
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