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Mol Cell Proteomics-2018-Liu-216-32.pdf 4,64MB
WeightNameValue
1000 Titel
  • The interactome of intact mitochondria by cross-linking mass spectrometry provides evidence for coexisting respiratory supercomplexes
1000 Autor/in
  1. Liu, Fan |
  2. Lössl, Philip |
  3. Rabbitts, Beverley M. |
  4. Balaban, Robert S. |
  5. Heck, Albert J.R. |
1000 Erscheinungsjahr 2017
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-12-08
1000 Erschienen in
1000 Quellenangabe
  • 17(2):216-232
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2018
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1074/mcp.RA117.000470 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795388/ |
1000 Ergänzendes Material
  • http://www.mcponline.org/content/17/2/216/suppl/DC1 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Mitochondria exert an immense amount of cytophysiological functions, but the structural basis of most of these processes is still poorly understood. Here we use cross-linking mass spectrometry to probe the organization of proteins in native mouse heart mitochondria. Our approach provides the largest survey of mitochondrial protein interactions reported so far. In total, we identify 3,322 unique residue-to-residue contacts involving half of the mitochondrial proteome detected by bottom-up proteomics. The obtained mitochondrial protein interactome gives insights in the architecture and submitochondrial localization of defined protein assemblies, and reveals the mitochondrial localization of four proteins not yet included in the MitoCarta database. As one of the highlights, we show that the oxidative phosphorylation complexes I-V exist in close spatial proximity, providing direct evidence for supercomplex assembly in intact mitochondria. The specificity of these contacts is demonstrated by comparative analysis of mitochondria after high salt treatment, which disrupts the native supercomplexes and substantially changes the mitochondrial interactome.
1000 Sacherschließung
lokal mitochondrial organization
lokal Protein-Protein Interactions*
lokal Crosslinking
lokal Macromolecular Assemblages*
lokal Macromolecular complex analysis
lokal Mitochondria function or biology
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TGl1LCBGYW4=|http://orcid.org/0000-0001-5062-7723|https://frl.publisso.de/adhoc/creator/UmFiYml0dHMsIEJldmVybGV5IE0u|https://frl.publisso.de/adhoc/creator/QmFsYWJhbiwgUm9iZXJ0IFMu|https://frl.publisso.de/adhoc/creator/SGVjaywgQWxiZXJ0IEouUi4=
1000 Label
1000 Förderer
  1. Netherlands Organisation for Scientific Research |
  2. European Union |
  3. Division of Intramural Research |
  4. National Heart Lung and Blood Institute |
  5. National Institutes of Health |
  6. U.S. Department of Health and Human Services |
1000 Fördernummer
  1. 184.032.201
  2. 686547; 317371
  3. -
  4. -
  5. -
  6. -
1000 Förderprogramm
  1. Proteins@Work; National Roadmap Large-Scale Research Facilities of the Netherlands
  2. European Union’s Horizon 2020 research and innovation program (MSMed); 7th framework program
  3. -
  4. -
  5. -
  6. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Netherlands Organisation for Scientific Research |
    1000 Förderprogramm Proteins@Work; National Roadmap Large-Scale Research Facilities of the Netherlands
    1000 Fördernummer 184.032.201
  2. 1000 joinedFunding-child
    1000 Förderer European Union |
    1000 Förderprogramm European Union’s Horizon 2020 research and innovation program (MSMed); 7th framework program
    1000 Fördernummer 686547; 317371
  3. 1000 joinedFunding-child
    1000 Förderer Division of Intramural Research |
    1000 Förderprogramm -
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer National Heart Lung and Blood Institute |
    1000 Förderprogramm -
    1000 Fördernummer -
  5. 1000 joinedFunding-child
    1000 Förderer National Institutes of Health |
    1000 Förderprogramm -
    1000 Fördernummer -
  6. 1000 joinedFunding-child
    1000 Förderer U.S. Department of Health and Human Services |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406698.rdf
1000 Erstellt am 2018-02-12T13:26:54.373+0100
1000 Erstellt von 241
1000 beschreibt frl:6406698
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Thu Jan 30 23:55:28 CET 2020
1000 Objekt bearb. Wed Jun 27 10:46:58 CEST 2018
1000 Vgl. frl:6406698
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406698 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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