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WeightNameValue
1000 Titel
  • Control of AMPA receptor activity by the extracellular loops of auxiliary proteins
1000 Autor/in
  1. Riva, Irene |
  2. Eibl, Clarissa |
  3. Volkmer, Rudolf |
  4. Carbone, Anna Lisa |
  5. Plested, Andrew |
1000 Erscheinungsjahr 2017
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-08-30
1000 Erschienen in
1000 Quellenangabe
  • 6: e28680
1000 FRL-Sammlung
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.7554/eLife.28680 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5599240/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • At synapses throughout the mammalian brain, AMPA receptors form complexes with auxiliary proteins, including TARPs. However, how TARPs modulate AMPA receptor gating remains poorly understood. We built structural models of TARP-AMPA receptor complexes for TARPs γ2 and γ8, combining recent structural studies and de novo structure predictions. These models, combined with peptide binding assays, provide evidence for multiple interactions between GluA2 and variable extracellular loops of TARPs. Substitutions and deletions of these loops had surprisingly rich effects on the kinetics of glutamate-activated currents, without any effect on assembly. Critically, by altering the two interacting loops of γ2 and γ8, we could entirely remove all allosteric modulation of GluA2, without affecting formation of AMPA receptor-TARP complexes. Likewise, substitutions in the linker domains of GluA2 completely removed any effect of γ2 on receptor kinetics, indicating a dominant role for this previously overlooked site proximal to the AMPA receptor channel gate.
1000 Sacherschließung
lokal desensitization
lokal none
lokal receptor kinetics
lokal modulation
lokal biophysics
lokal glutamate
lokal neuroscience
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/Uml2YSwgSXJlbmU=|https://frl.publisso.de/adhoc/creator/RWlibCwgQ2xhcmlzc2E=|https://frl.publisso.de/adhoc/creator/Vm9sa21lciwgUnVkb2xm|http://orcid.org/0000-0001-9746-2778|http://orcid.org/0000-0001-6062-0832
1000 Förderer
  1. German Research Foundation |
  2. Austrian Science fund (FWF) |
1000 Fördernummer
  1. FOR 2518; EXC-257
  2. J3682-B21
1000 Förderprogramm
  1. Funktionale Dynamik von Ionenkanälen und Transportern - DynIon -; Cluster of Excellence ‘NeuroCure’; Heisenberg Professor
  2. Erwin-Schrödinger Postdoctoral Fellowship
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Research Foundation |
    1000 Förderprogramm Funktionale Dynamik von Ionenkanälen und Transportern - DynIon -; Cluster of Excellence ‘NeuroCure’; Heisenberg Professor
    1000 Fördernummer FOR 2518; EXC-257
  2. 1000 joinedFunding-child
    1000 Förderer Austrian Science fund (FWF) |
    1000 Förderprogramm Erwin-Schrödinger Postdoctoral Fellowship
    1000 Fördernummer J3682-B21
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6406714.rdf
1000 Erstellt am 2018-02-14T10:44:29.355+0100
1000 Erstellt von 241
1000 beschreibt frl:6406714
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet Tue May 29 10:25:19 CEST 2018
1000 Objekt bearb. Tue May 29 10:25:09 CEST 2018
1000 Vgl. frl:6406714
1000 Oai Id
  1. oai:frl.publisso.de:frl:6406714 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
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