ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones

  1. Kawabe, Hiroshi
  2. Mitkovski, Miso
  3. Kaeser, Pascal S
  4. Hirrlinger, Johannes
  5. Opazo, Felipe ORCID logo
  6. Nestvogel, Dennis ORCID logo
  7. Kalla, Stefan ORCID logo
  8. Fejtova, Anna
  9. Verrier, Sophie ORCID logo
  10. Bungers, Simon ORCID logo
  11. Cooper, Benjamin H.
  12. Varoqueaux, Frederique ORCID logo
  13. Wang, Yun
  14. Nehring, Ralf B.
  15. Gundelfinger, Eckart ORCID logo
  16. Rosenmund, Christian
  17. Rizzoli, Silvio O.
  18. Südhof, Thomas C.
  19. Rhee, Jeong-Seop
  20. Brose, Nils ORCID logo

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WeightNameValue
1000 Titel
  • ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones
1000 Autor/in
  1. Kawabe, Hiroshi |
  2. Mitkovski, Miso |
  3. Kaeser, Pascal S |
  4. Hirrlinger, Johannes |
  5. Opazo, Felipe |
  6. Nestvogel, Dennis |
  7. Kalla, Stefan |
  8. Fejtova, Anna |
  9. Verrier, Sophie |
  10. Bungers, Simon |
  11. Cooper, Benjamin H. |
  12. Varoqueaux, Frederique |
  13. Wang, Yun |
  14. Nehring, Ralf B. |
  15. Gundelfinger, Eckart |
  16. Rosenmund, Christian |
  17. Rizzoli, Silvio O. |
  18. Südhof, Thomas C. |
  19. Rhee, Jeong-Seop |
  20. Brose, Nils |
1000 Erscheinungsjahr 2017
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-03-06
1000 Erschienen in
1000 Quellenangabe
  • 216(4):1143-1161
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1083/jcb.201606086 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379939/ |
1000 Ergänzendes Material
  • http://jcb.rupress.org/content/216/4/1143/tab-figures-data |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Presynaptic active zones (AZs) are unique subcellular structures at neuronal synapses, which contain a network of specific proteins that control synaptic vesicle (SV) tethering, priming, and fusion. Munc13s are core AZ proteins with an essential function in SV priming. In hippocampal neurons, two different Munc13s—Munc13-1 and bMunc13-2—mediate opposite forms of presynaptic short-term plasticity and thus differentially affect neuronal network characteristics. We found that most presynapses of cortical and hippocampal neurons contain only Munc13-1, whereas ∼10% contain both Munc13-1 and bMunc13-2. Whereas the presynaptic recruitment and activation of Munc13-1 depends on Rab3-interacting proteins (RIMs), we demonstrate here that bMunc13-2 is recruited to synapses by the AZ protein ELKS1, but not ELKS2, and that this recruitment determines basal SV priming and short-term plasticity. Thus, synapse-specific interactions of different Munc13 isoforms with ELKS1 or RIMs are key determinants of the molecular and functional heterogeneity of presynaptic AZs.
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/S2F3YWJlLCBIaXJvc2hp|https://frl.publisso.de/adhoc/creator/TWl0a292c2tpLCBNaXNv|https://frl.publisso.de/adhoc/creator/S2Flc2VyLCBQYXNjYWwgUw==|https://frl.publisso.de/adhoc/creator/SGlycmxpbmdlciwgSm9oYW5uZXM=|https://orcid.org/0000-0002-4968-9713|https://orcid.org/0000-0002-0568-2552|https://orcid.org/0000-0003-4496-5827|https://frl.publisso.de/adhoc/creator/RmVqdG92YSwgQW5uYQ==|https://orcid.org/0000-0003-1308-5229|https://orcid.org/0000-0002-2880-839X|https://frl.publisso.de/adhoc/creator/Q29vcGVyLCBCZW5qYW1pbiBILg==|https://orcid.org/0000-0002-3614-3121|https://frl.publisso.de/adhoc/creator/V2FuZywgWXVu|https://frl.publisso.de/adhoc/creator/TmVocmluZywgUmFsZiBCLg==|https://orcid.org/0000-0001-9377-7414|https://frl.publisso.de/adhoc/creator/Um9zZW5tdW5kLCBDaHJpc3RpYW4=|https://frl.publisso.de/adhoc/creator/Uml6em9saSwgU2lsdmlvIE8u|https://frl.publisso.de/adhoc/creator/U8O8ZGhvZiwgVGhvbWFzIEMu|https://frl.publisso.de/adhoc/creator/UmhlZSwgSmVvbmctU2VvcA==|https://orcid.org/0000-0003-0938-8534
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft
  2. European Union
  3. National Institutes of Health
  4. Fritz Thyssen Foundation
  5. Japan Society for the Promotion of Science
  6. Mochida Memorial Foundation for Medical and Pharmaceutical Research
1000 Fördernummer
  1. DFG-CNMPB FZT103, SPP1365/KA3423/1-1, KA3423/3-1, SFB958/A5
  2. -
  3. NS051262, R01NS083898
  4. -
  5. -
  6. -
1000 Förderprogramm
  1. -
  2. EUROSPIN, SynSys, European Research Council Advanced Grant SYNPRIME
  3. -
  4. -
  5. -
  6. -
1000 Dateien
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6407108.rdf
1000 Erstellt am 2018-03-14T10:11:10.355+0100
1000 Erstellt von 242
1000 beschreibt frl:6407108
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet 2020-01-30T23:58:14.066+0100
1000 Objekt bearb. Mon Mar 25 14:14:28 CET 2019
1000 Vgl. frl:6407108
1000 Oai Id
  1. oai:frl.publisso.de:frl:6407108 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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