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1000 Titel
  • Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence
1000 Autor/in
  1. Marthandan, Shiva |
  2. Menzel, Uwe |
  3. Priebe, Steffen |
  4. Groth, Marco |
  5. Guthke, Reinhard |
  6. Platzer, Matthias |
  7. Hemmerich, Peter |
  8. Kaether, Christoph |
  9. Diekmann, Stephan |
1000 Erscheinungsjahr 2016
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-07-28
1000 Erschienen in
1000 Quellenangabe
  • 49:34
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2016
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1186/s40659-016-0095-2 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/27464526/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Cellular senescence is induced either internally, for example by replication exhaustion and cell division, or externally, for example by irradiation. In both cases, cellular damages accumulate which, if not successfully repaired, can result in senescence induction. Recently, we determined the transcriptional changes combined with the transition into replicative senescence in primary human fibroblast strains. Here, by γ-irradiation we induced premature cellular senescence in the fibroblast cell strains (HFF and MRC-5) and determined the corresponding transcriptional changes by high-throughput RNA sequencing. RESULTS: Comparing the transcriptomes, we found a high degree of similarity in differential gene expression in replicative as well as in irradiation induced senescence for both cell strains suggesting, in each cell strain, a common cellular response to error accumulation. On the functional pathway level, “Cell cycle” was the only pathway commonly down-regulated in replicative and irradiation-induced senescence in both fibroblast strains, confirming the tight link between DNA repair and cell cycle regulation. However, “DNA repair” and “replication” pathways were down-regulated more strongly in fibroblasts undergoing replicative exhaustion. We also retrieved genes and pathways in each of the cell strains specific for irradiation induced senescence. CONCLUSION: We found the pathways associated with “DNA repair” and “replication” less stringently regulated in irradiation induced compared to replicative senescence. The strong regulation of these pathways in replicative senescence highlights the importance of replication errors for its induction.
1000 Sacherschließung
lokal Senescence
lokal Transcriptome analysis
lokal Aging
lokal Fibroblasts
lokal DNA repair
lokal γ-irradiation
1000 Fachgruppe
  1. Medizin |
  2. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TWFydGhhbmRhbiwgU2hpdmE=|https://frl.publisso.de/adhoc/creator/TWVuemVsLCBVd2U=|https://frl.publisso.de/adhoc/creator/UHJpZWJlLCBTdGVmZmVu|http://orcid.org/0000-0002-9199-8990|https://frl.publisso.de/adhoc/creator/R3V0aGtlLCBSZWluaGFyZA==|http://orcid.org/0000-0003-0596-8582|https://frl.publisso.de/adhoc/creator/SGVtbWVyaWNoLCBQZXRlcg==|https://frl.publisso.de/adhoc/creator/S2FldGhlciwgQ2hyaXN0b3Bo|https://frl.publisso.de/adhoc/creator/RGlla21hbm4sIFN0ZXBoYW4=
1000 Label
1000 Förderer
  1. German Ministry for Education and Research (BMBF) |
1000 Fördernummer
  1. 0315581
1000 Förderprogramm
  1. JenAge
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer German Ministry for Education and Research (BMBF) |
    1000 Förderprogramm JenAge
    1000 Fördernummer 0315581
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6407357.rdf
1000 Erstellt am 2018-03-29T10:59:41.546+0200
1000 Erstellt von 285
1000 beschreibt frl:6407357
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet Thu Jan 30 18:55:14 CET 2020
1000 Objekt bearb. Tue Apr 03 09:55:53 CEST 2018
1000 Vgl. frl:6407357
1000 Oai Id
  1. oai:frl.publisso.de:frl:6407357 |
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1000 Sichtbarkeit Daten public
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