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1000 Titel
  • Lactate promotes specific differentiation in bovine granulosa cells depending on lactate uptake thus mimicking an early post-LH stage
1000 Autor/in
  1. Baufeld, Anja |
  2. Vanselow, Jens |
1000 Erscheinungsjahr 2018
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2018-02-20
1000 Erschienen in
1000 Quellenangabe
  • 16:15
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2018
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1186/s12958-018-0332-3 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5819637/ |
1000 Ergänzendes Material
  • https://rbej.biomedcentral.com/articles/10.1186/s12958-018-0332-3#Declarations |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: The LH-induced folliculo-luteal transformation is connected with alterations of the gene expression profile in cells of the granulosa layer. It has been described that hypoxic conditions occur during luteinization, thus favoring the formation of L-lactate within the follicle. Despite being a product of anaerobic respiration, L-lactate has been shown to act as a signaling molecule affecting gene expression in neuronal cells. During the present study, we tested the hypothesis that L-lactate may influence differentiation of follicular granulosa cells (GC). METHODS: In a bovine granulosa cell culture model effects of L- and D-lactate, of increased glucose concentrations and of the lactate transport inhibitor UK5099 were analyzed. Steroid hormone production was analyzed by RIA and the abundance of key transcripts was determined by quantitative real-time RT-PCR. RESULTS: L-lactate decreased the production of estradiol and significantly affected selected genes of the folliculo-luteal transition as well as genes of the lactate metabolism. CYP19A1, FSHR, LHCGR were down-regulated, whereas RGS2, VNN2, PTX3, LDHA and lactate transporters were up-regulated. These effects could be partly or completely reversed by pre-treatment of the cells with UK5099. The non-metabolized enantiomer D-lactate had even more pronounced effects on gene expression, whereas increased glucose concentrations did not affect transcript abundance. CONCLUSIONS: In summary, our data suggest that L-lactate specifically alters physiological and molecular characteristics of GC. These effects critically depend on L-lactate uptake, but are not triggered by increased energy supply. Further, we could show that L-lactate has a positive feedback on the lactate metabolism. Therefore, we hypothesize that L-lactate acts as a signaling molecule in bovine and possibly other monovular species supporting differentiation during the folliculo-luteal transformation.
1000 Sacherschließung
lokal Bovine
lokal Expression
lokal Granulosa cells
lokal L-lactate
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/QmF1ZmVsZCwgQW5qYQ==|http://orcid.org/0000-0001-7374-4615
1000 Label
1000 Förderer
  1. Leibniz Institute for Farm Animal Biology (FBN)
1000 Fördernummer
  1. -
1000 Förderprogramm
  1. -
1000 Dateien
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6407992.rdf
1000 Erstellt am 2018-05-09T07:26:43.206+0200
1000 Erstellt von 25
1000 beschreibt frl:6407992
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Thu Jan 30 21:31:44 CET 2020
1000 Objekt bearb. Wed May 09 07:27:24 CEST 2018
1000 Vgl. frl:6407992
1000 Oai Id
  1. oai:frl.publisso.de:frl:6407992 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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