Age-dependent increase of oxidative stress regulates microRNA-29 family preserving cardiac health

  1. Heid, Johanna
  2. Cencioni, Chiara
  3. Ripa, Roberto
  4. Baumgart, Mario ORCID logo
  5. Atlante, Sandra ORCID logo
  6. Milano, Giuseppina ORCID logo
  7. Scopece, Alessandro ORCID logo
  8. Kuenne, Carsten ORCID logo
  9. Guenther, Stefan
  10. Azzimato, Valerio
  11. Farsetti, Antonella
  12. Rossi, Giacomo
  13. Braun, Thomas ORCID logo
  14. Pompilio, Giulio ORCID logo
  15. Martelli, Fabio ORCID logo
  16. Zeiher, Andreas ORCID logo
  17. Cellerino, Alessandro ORCID logo
  18. Gaetano, Carlo ORCID logo
  19. Spallotta, Francesco ORCID logo

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1000 Titel
  • Age-dependent increase of oxidative stress regulates microRNA-29 family preserving cardiac health
1000 Autor/in
  1. Heid, Johanna |
  2. Cencioni, Chiara |
  3. Ripa, Roberto |
  4. Baumgart, Mario |
  5. Atlante, Sandra |
  6. Milano, Giuseppina |
  7. Scopece, Alessandro |
  8. Kuenne, Carsten |
  9. Guenther, Stefan |
  10. Azzimato, Valerio |
  11. Farsetti, Antonella |
  12. Rossi, Giacomo |
  13. Braun, Thomas |
  14. Pompilio, Giulio |
  15. Martelli, Fabio |
  16. Zeiher, Andreas |
  17. Cellerino, Alessandro |
  18. Gaetano, Carlo |
  19. Spallotta, Francesco |
1000 Erscheinungsjahr 2017
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-12-04
1000 Erschienen in
1000 Quellenangabe
  • 7:16839
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1038/s41598-017-16829-w |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/29203887/ |
1000 Ergänzendes Material
  • https://www.nature.com/articles/s41598-017-16829-w#Sec22 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The short-lived turquoise killifish Nothobranchius furzeri (Nfu) is a valid model for aging studies. Here, we investigated its age-associated cardiac function. We observed oxidative stress accumulation and an engagement of microRNAs (miRNAs) in the aging heart. MiRNA-sequencing of 5 week (young), 12–21 week (adult) and 28–40 week (old) Nfu hearts revealed 23 up-regulated and 18 down-regulated miRNAs with age. MiR-29 family turned out as one of the most up-regulated miRNAs during aging. MiR-29 family increase induces a decrease of known targets like collagens and DNA methyl transferases (DNMTs) paralleled by 5´methyl-cytosine (5mC) level decrease. To further investigate miR-29 family role in the fish heart we generated a transgenic zebrafish model where miR-29 was knocked-down. In this model we found significant morphological and functional cardiac alterations and an impairment of oxygen dependent pathways by transcriptome analysis leading to hypoxic marker up-regulation. To get insights the possible hypoxic regulation of miR-29 family, we exposed human cardiac fibroblasts to 1% O2 levels. In hypoxic condition we found miR-29 down-modulation responsible for the accumulation of collagens and 5mC. Overall, our data suggest that miR-29 family up-regulation might represent an endogenous mechanism aimed at ameliorating the age-dependent cardiac damage leading to hypertrophy and fibrosis.
1000 Sacherschließung
lokal Cell biology
lokal Cardiovascular biology
1000 Fachgruppe
  1. Medizin |
  2. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/SGVpZCwgSm9oYW5uYQ==|https://frl.publisso.de/adhoc/creator/Q2VuY2lvbmksIENoaWFyYQ==|https://frl.publisso.de/adhoc/creator/UmlwYSwgUm9iZXJ0bw==|http://orcid.org/0000-0001-8172-1595|http://orcid.org/0000-0001-8090-9895|http://orcid.org/0000-0002-5560-4667|http://orcid.org/0000-0001-5883-484X|http://orcid.org/0000-0001-8013-5906|https://frl.publisso.de/adhoc/creator/R3VlbnRoZXIsIFN0ZWZhbg==|https://frl.publisso.de/adhoc/creator/QXp6aW1hdG8sIFZhbGVyaW8=|https://frl.publisso.de/adhoc/creator/RmFyc2V0dGksIEFudG9uZWxsYQ==|https://frl.publisso.de/adhoc/creator/Um9zc2ksIEdpYWNvbW8=|http://orcid.org/0000-0002-6165-4804|http://orcid.org/0000-0003-2581-5735|http://orcid.org/0000-0002-8624-7738|http://orcid.org/0000-0003-1711-5819|http://orcid.org/0000-0003-3834-0097|http://orcid.org/0000-0002-5238-1832|http://orcid.org/0000-0002-0466-6286
1000 Förderer
  1. Hessische Ministerium für Wissenschaft und Kunst
  2. Deutsche Forschungsgemeinschaft (DFG)
  3. August Scheidel-Stiftung
  4. Amandus und Barbara Pauli Stiftung
  5. Fondazione Pisa
1000 Fördernummer
  1. III L 5–518/17.004
  2. -
  3. -
  4. -
  5. -
1000 Förderprogramm
  1. LOEWE Cell & Gene Therapy Center (LOEWE-CGT)
  2. SFB834 “Endothelial Signaling and Vascular Repair”; Project B11; Excellence Cluster Cardio Pulmonary System
  3. -
  4. -
  5. ETHERNA
1000 Dateien
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6408436.rdf
1000 Erstellt am 2018-06-19T10:19:16.556+0200
1000 Erstellt von 285
1000 beschreibt frl:6408436
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Wed Jun 20 11:54:31 CEST 2018
1000 Objekt bearb. Wed Jun 20 11:54:16 CEST 2018
1000 Vgl. frl:6408436
1000 Oai Id
  1. oai:frl.publisso.de:frl:6408436 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
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