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1000 Titel
  • Artificial Polysialic Acid Chains as Sialidase-Resistant Molecular-Anchors to Accumulate Particles on Neutrophil Extracellular Traps
1000 Autor/in
  1. Galuska, Christina E. |
  2. Dambon, Jan A. |
  3. Kühnle, Andrea |
  4. Bornhöfft, Kim F. |
  5. Prem, Gerlinde |
  6. Zlatina, Kristina |
  7. Lütteke, Thomas |
  8. Galuska, Sebastian P. |
1000 Erscheinungsjahr 2017
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-09-29
1000 Erschienen in
1000 Quellenangabe
  • 8:1229
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.3389/fimmu.2017.01229 |
  • https://www.ncbi.nlm.nih.gov/pubmed/29033944 |
1000 Ergänzendes Material
  • https://www.frontiersin.org/articles/10.3389/fimmu.2017.01229/full#h11 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Neutrophils are involved in numerous immunological events. One mechanism of neutrophils to combat pathogens is the formation of neutrophil extracellular traps (NETs). Thereby, neutrophils use DNA fibers to form a meshwork of DNA and histones as well as several antimicrobial components to trap and kill invaders. However, the formation of NETs can lead to pathological conditions triggering among other things (e.g., sepsis or acute lung failure), which is mainly a consequence of the cytotoxic characteristics of accumulated extracellular histones. Interestingly, the carbohydrate polysialic acid represents a naturally occurring antagonist of the cytotoxic properties of extracellular histones. Inspired by polysialylated vesicles, we developed polysialylated nanoparticles. Since sialidases are frequently present in areas of NET formation, we protected the sensitive non-reducing end of these homopolymers. To this end, the terminal sialic acid residue of the non-reducing end was oxidized and directly coupled to nanoparticles. The covalently linked sialidase-resistant polysialic acid chains are still able to neutralize histone-mediated cytotoxicity and to initiate binding of these polysialylated particles to NET filaments. Furthermore, polysialylated fluorescent microspheres can be used as a bioanalytical tool to stain NET fibers. Thus, polySia chains might not only be a useful agent to reduce histone-mediated cytotoxicity but also an anchor to accumulate nanoparticles loaded with active substances in areas of NET formation.
1000 Sacherschließung
lokal histones
lokal lipopolysaccharides
lokal neutrophil extracellular traps
lokal polysialic acid
lokal nanoparticle
1000 Fachgruppe
  1. Biologie |
  2. Medizin |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/R2FsdXNrYSwgQ2hyaXN0aW5hIEUu|https://frl.publisso.de/adhoc/creator/RGFtYm9uLCBKYW4gQS4=|https://frl.publisso.de/adhoc/creator/S8O8aG5sZSwgQW5kcmVh|https://frl.publisso.de/adhoc/creator/Qm9ybmjDtmZmdCwgS2ltIEYu|https://frl.publisso.de/adhoc/creator/UHJlbSwgR2VybGluZGU=|http://orcid.org/0000-0003-2912-3445|https://frl.publisso.de/adhoc/creator/TMO8dHRla2UsIFRob21hcw==|http://orcid.org/0000-0001-5565-0252
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
  2. Behring-Roentgen-Stiftung |
  3. Leibniz Institute for Farm Animal Biology (FBN) |
1000 Fördernummer
  1. GA 1755/1-2
  2. 60-008
  3. -
1000 Förderprogramm
  1. -
  2. -
  3. Open Access Fund
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer GA 1755/1-2
  2. 1000 joinedFunding-child
    1000 Förderer Behring-Roentgen-Stiftung |
    1000 Förderprogramm -
    1000 Fördernummer 60-008
  3. 1000 joinedFunding-child
    1000 Förderer Leibniz Institute for Farm Animal Biology (FBN) |
    1000 Förderprogramm Open Access Fund
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6410257.rdf
1000 Erstellt am 2018-09-18T09:39:49.943+0200
1000 Erstellt von 122
1000 beschreibt frl:6410257
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet Tue Sep 18 09:41:14 CEST 2018
1000 Objekt bearb. Tue Sep 18 09:40:51 CEST 2018
1000 Vgl. frl:6410257
1000 Oai Id
  1. oai:frl.publisso.de:frl:6410257 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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