fphys-09-01066.pdf 2,91MB
1000 Titel
  • Low Oxygen Levels Induce Early Luteinization Associated Changes in Bovine Granulosa Cells
1000 Autor/in
  1. Baddela, Vijay S. |
  2. Sharma, Arpna |
  3. Viergutz, Torsten |
  4. Koczan, Dirk |
  5. Vanselow, Jens |
1000 Erscheinungsjahr 2018
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2018-08-07
1000 Erschienen in
1000 Quellenangabe
  • 9:1066
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2018
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Ergänzendes Material
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • During follicle maturation, oxygen levels continuously decrease in the follicular fluid and reach lowest levels in the preovulatory follicle. The current study was designed to comprehensively understand effects of low oxygen levels on bovine granulosa cells (GC) using our established estrogen active GC culture model. As evident from flow cytometry analysis the viability of GC was not found to be affected at severely low oxygen condition (1% O2) compared to normal (atmospheric) oxygen condition (21% O2). Estimations of hormone concentrations using competitive radioimmunoassay revealed that the production of estradiol and progesterone was significantly reduced at low oxygen condition. To understand the genome-wide changes of gene expression, mRNA microarray analysis was performed using Affymetrix’s Bovine Gene 1.0 ST Arrays. This resulted in the identification of 1104 differentially regulated genes of which 505 were up- and 599 down-regulated under low oxygen conditions. Pathway analysis using Ingenuity pathway analyzer (IPA) identified 36 significantly affected (p < 0.05) canonical pathways. Importantly, pathways like “Estrogen-mediated S-phase Entry” and “Cyclins and Cell Cycle Regulation” were found to be greatly down-regulated at low oxygen levels. This was experimentally validated using flow cytometry based cell cycle analysis. Up-regulation of critical genes associated with angiogenesis, inflammation, and glucose metabolism, and down-regulation of FSH signaling, steroidogenesis and cell proliferation indicated that low oxygen levels induced early luteinization associated changes in granulosa cells. Identification of unmethylated CpG sites in the CYP19A1 promoter region suggests that granulosa cells were not completely transformed into luteal cells under the present low oxygen in vitro condition. In addition, the comparison with earlier published in vivo microarray data indicated that 1107 genes showed a similar expression pattern in granulosa cells at low oxygen levels (in vitro) as found in preovulatory follicles after the LH surge (in vivo). Overall, our findings demonstrate for the first time that low oxygen levels in preovulatory follicles may play an important role in supporting early events of luteinization in granulosa cells.
1000 Sacherschließung
lokal preovulatory follicle
lokal luteinization
lokal granulosa cells
lokal oxygen levels
lokal gene expression
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Label
1000 Förderer
  1. Alexander von Humboldt foundation |
1000 Fördernummer
  1. -
1000 Förderprogramm
  1. fellowship
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Alexander von Humboldt foundation |
    1000 Förderprogramm fellowship
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6410259.rdf
1000 Erstellt am 2018-09-18T09:51:54.666+0200
1000 Erstellt von 122
1000 beschreibt frl:6410259
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet Thu Jan 30 19:15:01 CET 2020
1000 Objekt bearb. Tue Sep 18 09:52:41 CEST 2018
1000 Vgl. frl:6410259
1000 Oai Id
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1000 Sichtbarkeit Metadaten public
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