WeightNameValue
1000 Titel
  • Expression of the Prion Protein Gene (PRNP) and Cellular Prion Protein (PrPc) in Cattle and Sheep Fetuses and Maternal Tissues During Pregnancy
1000 Autor/in
  1. Thumdee, Patama |
  2. Ponsuksili, Siriluck |
  3. Muráni, Eduard |
  4. Nganvongpanit, Korakot |
  5. Gehrig, Bernhard |
  6. Tesfaye, Dawit |
  7. Hoelker, Michael |
  8. Jennen, Danyel |
  9. Griese, Josef |
  10. Schellander, Karl |
  11. Wimmers, Klaus |
1000 Erscheinungsjahr 2018
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2018-07-05
1000 Erschienen in
1000 Quellenangabe
  • 13(4-5): 283-297
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032460/ |
  • https://www.cognizantcommunication.com/cccSiteFiles/Gene/gene13abs4.html#gene13abs46 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • We investigated the expression of prion protein gene both on mRNA and protein levels in bovine and ovine female reproductive organs during gestation and various tissues of their fetuses. The fetal tissues of both species included brain, cotyledon, heart, intestine, kidney, liver, lung, and muscle. In cattle, prion protein gene (PRNP) transcripts were detected by semiquantitative RT-PCR in reproductive tissues such as ovary, oviduct, endometrium, myometrium, follicles, and granulosa cells. In various tissues of 2-month-old fetuses, higher expression levels were found in brain and cotyledon compared to the other tissues. To detect the expression of the gene transcript in in vivo preimplantation embryos and 1-month-old fetuses, real-time PCR was performed showing that the level of gene expression in zygote stage was significantly higher (p ≤ 0.05) than that of the other stages. Sheep were categorized as resistant (R1) or high susceptible (R5) to scrapie according to their PRNP genotype. In both genotype groups, the PRNP mRNA was detectable in all tissues studied including ovary, oviduct, endometrium, myometrium, and caruncle of ewes and all tissues of 2-month-old fetuses of both groups. Comparison between reproductive organs demonstrates the highest expression level in caruncle tissue of R1 ewes, whereas the level was high in brain and low in liver of both R1 and R5 fetuses. In addition, real-time RT-PCR was performed in immature oocytes, mature oocytes, in vivo embryos at morula stage, and 1-month-old fetuses. The results showed that the relative expression levels of the ovine PRNP mRNA in mature oocytes and morula stage embryos were significantly lower than those in immature oocytes and 1-month-old fetuses (p ≤ 0.05). Western blot analyses revealed the immunoreactive bands corresponding to the cellular prion protein (PrPc) in all maternal and fetal tissues examined of both cattle and sheep. Moreover, immunohistochemical staining implicated localization of the PrPc in ovarian cortex and ovarian medulla of both species. However, PrPc was not detected in oocyte, granulosa cells, theca cells, and corpus luteum in this study.
1000 Sacherschließung
lokal Pregnancy
lokal Prion protein gene (PRNP)
lokal Fetus
lokal Prion
lokal Reproductive organs
lokal Preimplantation embryo
lokal Cellular prion protein (PrPc)
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/VGh1bWRlZSwgUGF0YW1h|https://frl.publisso.de/adhoc/creator/UG9uc3Vrc2lsaSwgU2lyaWx1Y2s=|https://frl.publisso.de/adhoc/creator/TXVyw6FuaSwgRWR1YXJk|https://frl.publisso.de/adhoc/creator/TmdhbnZvbmdwYW5pdCwgS29yYWtvdA==|https://frl.publisso.de/adhoc/creator/R2VocmlnLCBCZXJuaGFyZA==|https://frl.publisso.de/adhoc/creator/VGVzZmF5ZSwgRGF3aXQ=|https://frl.publisso.de/adhoc/creator/SG9lbGtlciwgTWljaGFlbA==|https://frl.publisso.de/adhoc/creator/SmVubmVuLCBEYW55ZWw=|https://frl.publisso.de/adhoc/creator/R3JpZXNlLCBKb3NlZg==|https://frl.publisso.de/adhoc/creator/U2NoZWxsYW5kZXIsIEthcmw=|http://orcid.org/0000-0002-9523-6790
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1000 Erstellt am 2018-10-08T15:51:44.457+0200
1000 Erstellt von 122
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1000 Zuletzt bearbeitet Mon Oct 08 15:52:46 CEST 2018
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1000 Vgl. frl:6410425
1000 Oai Id
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