WeightNameValue
1000 Titel
  • Desialylation of Spermatozoa and Epithelial Cell Glycocalyx Is a Consequence of Bacterial Infection of the Epididymis
1000 Autor/in
  1. Khosravi, Farhad |
  2. Michel, Vera |
  3. Galuska, Christina E. |
  4. Bhushan, Sudhanshu |
  5. Christian, Philipp |
  6. Schuppe, Hans-Christian |
  7. Pilatz, Adrian |
  8. Galuska, Sebastian P. |
  9. Meinhardt, Andreas |
1000 Erscheinungsjahr 2016
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2016-06-23
1000 Erschienen in
1000 Quellenangabe
  • 91(34): 17717-17726
1000 FRL-Sammlung
1000 Verlagsversion
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5016166/ |
  • https://dx.doi.org/10.1074/jbc.M116.718072 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Urinary tract infections caused by uropathogenic Escherichia coli (UPEC) pathovars belong to the most frequent infections in humans. In men, pathogens can also spread to the genital tract via the continuous ductal system, eliciting bacterial prostatitis and/or epididymo-orchitis. Antibiotic treatment usually clears pathogens in acute epididymitis; however, the fertility of patients can be permanently impaired. Because a premature acrosome reaction was observed in an UPEC epididymitis mouse model, and sialidases on the sperm surface are considered to be activated via proteases of the acrosome, we aimed to investigate whether alterations of the sialome of epididymal spermatozoa and surrounding epithelial cells occur during UPEC infection. In UPEC-elicited acute epididymitis in mice, a substantial loss of N-acetylneuraminic acid residues was detected in epididymal spermatozoa and epithelial cells using combined laser microdissection/HPLC-ESI-MS analysis. In support, a substantial reduction of sialic acid residues bound to the surface of spermatozoa was documented in men with a recent history of E. coli-associated epididymitis. In vitro, such an UPEC induced N-acetylneuraminic acid release from human spermatozoa was effectively counteracted by a sialidase inhibitor. These findings strongly suggest a substantial remodeling of the glycocalyx of spermatozoa and epididymal epithelial cells by endogenous sialidases after a premature acrosome reaction during acute epididymitis.
1000 Sacherschließung
lokal sialidase
lokal spermatozoa
lokal sialic acid
lokal glycosylation
lokal sperm
lokal glycosylation inhibitor
lokal bacterial pagthogenesis
lokal reproduction
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/S2hvc3JhdmksIEZhcmhhZA==|https://frl.publisso.de/adhoc/creator/TWljaGVsLCBWZXJh|https://frl.publisso.de/adhoc/creator/R2FsdXNrYSwgQ2hyaXN0aW5hIEUu|https://frl.publisso.de/adhoc/creator/Qmh1c2hhbiwgU3VkaGFuc2h1|https://frl.publisso.de/adhoc/creator/Q2hyaXN0aWFuLCBQaGlsaXBw|https://frl.publisso.de/adhoc/creator/U2NodXBwZSwgSGFucy1DaHJpc3RpYW4=|https://frl.publisso.de/adhoc/creator/UGlsYXR6LCBBZHJpYW4=|http://orcid.org/0000-0001-5565-0252|https://frl.publisso.de/adhoc/creator/TWVpbmhhcmR0LCBBbmRyZWFz
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
  2. von Behring-Roentgen-Stiftung |
  3. Faculty of Medicine of the Justus Liebig University of Giessen |
1000 Fördernummer
  1. GA 1755/1-2; GRK 1871/1
  2. -
  3. -
1000 Förderprogramm
  1. -
  2. -
  3. -
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer GA 1755/1-2; GRK 1871/1
  2. 1000 joinedFunding-child
    1000 Förderer von Behring-Roentgen-Stiftung |
    1000 Förderprogramm -
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Faculty of Medicine of the Justus Liebig University of Giessen |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6410465.rdf
1000 Erstellt am 2018-10-09T13:09:15.194+0200
1000 Erstellt von 122
1000 beschreibt frl:6410465
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet Wed Oct 17 11:17:40 CEST 2018
1000 Objekt bearb. Wed Oct 17 11:17:40 CEST 2018
1000 Vgl. frl:6410465
1000 Oai Id
  1. oai:frl.publisso.de:frl:6410465 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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