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1000 Titel
  • A Tissue Culture Model of Estrogen-producing Primary Bovine Granulosa Cells
1000 Autor/in
  1. Baufeld, Anja |
  2. Vanselow, Jens |
1000 Erscheinungsjahr 2018
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2018-09-06
1000 Erschienen in
1000 Quellenangabe
  • 139:e58208
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2018
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.3791/58208 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6235104/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Ovarian granulosa cells (GC) are the major source of estradiol synthesis. Induced by the preovulatory luteinizing hormone (LH) surge, cells of the theca and, in particular, of the granulosa cell layer profoundly change their morphological, physiological, and molecular characteristics and form the progesterone-producing corpus luteum that is responsible for maintaining pregnancy. Cell culture models are essential tools to study the underlying regulatory mechanisms involved in the folliculo-luteal transformation. The presented protocol focuses on the isolation procedure and cryopreservation of bovine GC from small- to medium-sized follicles (< 6 mm). With this technique, a nearly pure population of GC can be obtained. The cryopreservation procedure greatly facilitates time management of the cell culture work independent of a direct primary tissue (ovaries) supply. This protocol describes a serum-free cell culture model that mimics the estradiol-active status of bovine GC. Important conditions that are essential for a successful steroid-active cell culture are discussed throughout the protocol. It is demonstrated that increasing the plating density of the cells induces a specific response as indicated by an altered gene expression profile and hormone production. Furthermore, this model provides a basis for further studies on GC differentiation and other applications.
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  1. https://frl.publisso.de/adhoc/creator/QmF1ZmVsZCwgQW5qYQ==|http://orcid.org/0000-0001-7374-4615
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1000 Erstellt am 2018-10-16T10:10:17.397+0200
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