Download
Miersch2018_Article_EffectsOfTrypsinizationAndOfAC.pdf 5,04MB
WeightNameValue
1000 Titel
  • Effects of trypsinization and of a combined trypsin, collagenase, and DNase digestion on liberation and in vitro function of satellite cells isolated from juvenile porcine muscles
1000 Autor/in
  1. Miersch, Claudia |
  2. Stange, Katja |
  3. Röntgen, Monika |
1000 Erscheinungsjahr 2018
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2018-05-21
1000 Erschienen in
1000 Quellenangabe
  • 54(6): 406-412
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2018
1000 Lizenz
1000 Verlagsversion
  • https://dx.doi.org/10.1007/s11626-018-0263-5 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5997727/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Muscle stem cells, termed satellite cells (SC), and SC-derived myogenic progenitor cells (MPC) are involved in postnatal muscle growth, regeneration, and muscle adaptability. They can be released from their natural environment by mechanical disruption and tissue digestion. The literature contains several isolation protocols for porcine SC/MPC including various digestion procedures, but comparative studies are missing. In this report, classic trypsinization and a more complex trypsin, collagenase, and DNase (TCD) digestion were performed with skeletal muscle tissue from 4- to 5-d-old piglets. The two digestion procedures were compared regarding cell yield, viability, myogenic purity, and in vitro cell function. The TCD digestion tended to result in higher cell yields than digestion with solely trypsin (statistical trend p = 0.096), whereas cell size and viability did not differ. Isolated myogenic cells from both digestion procedures showed comparable proliferation rates, expressed the myogenic marker Desmin, and initiated myogenic differentiation in vitro at similar levels. Thus, TCD digestion tended to liberate slightly more cells without changes in the tested in vitro properties of the isolated cells. Both procedures are adequate for the isolation of SC/MPC from juvenile porcine muscles but the developmental state of the animal should always be considered.
1000 Sacherschließung
lokal Trypsin
lokal Myogenic differentiation
lokal Collagenase
lokal Muscle tissue
lokal Pig
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TWllcnNjaCwgQ2xhdWRpYQ==|http://orcid.org/0000-0002-8416-2204|https://frl.publisso.de/adhoc/creator/UsO2bnRnZW4sIE1vbmlrYQ==
1000 Förderer
  1. Leibniz Institute for Farm Animal Biology (FBN) |
1000 Fördernummer
  1. -
1000 Förderprogramm
  1. Future Fund; Open Access Fund
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Leibniz Institute for Farm Animal Biology (FBN) |
    1000 Förderprogramm Future Fund; Open Access Fund
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6410600.rdf
1000 Erstellt am 2018-10-16T11:02:45.909+0200
1000 Erstellt von 122
1000 beschreibt frl:6410600
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet Wed Oct 17 11:30:32 CEST 2018
1000 Objekt bearb. Wed Oct 17 11:30:31 CEST 2018
1000 Vgl. frl:6410600
1000 Oai Id
  1. oai:frl.publisso.de:frl:6410600 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

View source