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1000 Titel
  • Transcriptional profiling of liver in riboflavin-deficient chicken embryos explains impaired lipid utilization, energy depletion, massive hemorrhaging, and delayed feathering
1000 Autor/in
  1. Cogburn, Larry A. |
  2. Smarsh, Danielle N. |
  3. Wang, Xiaofei |
  4. Trakooljul, Nares |
  5. Carré, Wilfrid |
  6. White, Harold B. |
1000 Erscheinungsjahr 2018
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2018-05-05
1000 Erschienen in
1000 Quellenangabe
  • 19:177
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2018
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Ergänzendes Material
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: A strain of Leghorn chickens (rd/rd), unable to produce a functional riboflavin-binding protein, lays riboflavin-deficient eggs, in which all embryos suddenly die at mid-incubation (days 13-15). This malady, caused by riboflavin deficiency, leads to excessive lipid accumulation in liver, impaired β-oxidation of lipid, and severe hypoglycemia prior to death. We have used high-density chicken microarrays for time-course transcriptional scans of liver in chicken embryos between days 9-15 during this riboflavin-deficiency-induced metabolic catastrophe. For comparison, half of rd/rd embryos (n = 16) were rescued from this calamity by injection of riboflavin just prior to incubation of fertile eggs from rd/rd hens. RESULTS: No significant differences were found between hepatic transcriptomes of riboflavin-deficient and riboflavin-rescued embryos at the first two ages (days 9 and 11). Overall, we found a 3.2-fold increase in the number of differentially expressed hepatic genes between day 13 (231 genes) and day 15 (734 genes). Higher expression of genes encoding the chicken flavoproteome was more evident in rescued- (15 genes) than in deficient-embryos (4 genes) at day 15. Diminished activity of flavin-dependent enzymes in riboflavin-deficient embryos blocks catabolism of yolk lipids, which normally serves as the predominant source of energy required for embryonic development. CONCLUSIONS: Riboflavin deficiency in mid-stage embryos leads to reduced expression of numerous genes controlling critical functions, including β-oxidation of lipids, blood coagulation and feathering. Surprisingly, reduced expression of feather keratin 1 was found in liver of riboflavin-deficient embryos at e15, which could be related to their delayed feathering and sparse clubbed down. A large number of genes are expressed at higher levels in liver of riboflavin-deficient embryos; these up-regulated genes control lipid storage/transport, gluconeogenesis, ketogenesis, protein catabolism/ubiquitination and cell death.
1000 Sacherschließung
lokal Coagulation factors
lokal Expression profiling
lokal Riboflavin deficiency
lokal Protease inhibitors
lokal Sudden embryonic death
lokal Impaired lipid metabolism
lokal β-oxidation
lokal Chicken flavoproteome
lokal Acetyl CoA deficiency
lokal Feather keratin
lokal Microarray analysis
lokal Protein catabolism
1000 Fachgruppe
  1. Agrarwissenschaften |
  2. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Label
1000 Förderer
  1. United States Department of Agriculture, Cooperative State Research, Education, and Extension Service, Initiative for Future Agriculture and Food Systems (USDA-CREES-IFAFS)
  2. USDA-National Research Initiative (NRI)
  3. Department of Animal and Food Sciences, University of Delaware
1000 Fördernummer
  1. 00-52,100-9614
  2. #2005-35206-15288
  3. -
1000 Förderprogramm
  1. -
  2. -
  3. Publication cost
1000 Dateien
1000 Objektart article
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1000 @id frl:6410731.rdf
1000 Erstellt am 2018-10-23T11:17:13.014+0200
1000 Erstellt von 122
1000 beschreibt frl:6410731
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Thu Jan 30 21:56:00 CET 2020
1000 Objekt bearb. Thu Jan 17 13:31:21 CET 2019
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