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1000 Titel
  • Muscle Transcriptional Profile Based on Muscle Fiber, Mitochondrial Respiratory Activity, and Metabolic Enzymes
1000 Autor/in
  1. Liu, Xuan |
  2. Du, Yang |
  3. Trakooljul, Nares |
  4. Brand, Bodo |
  5. Muráni, Eduard |
  6. Krischek, Carsten |
  7. Wicke, Michael |
  8. Schwerin, Manfred |
  9. Wimmers, Klaus |
  10. Ponsuksili, Siriluck |
1000 Erscheinungsjahr 2015
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2015-11-1
1000 Erschienen in
1000 Quellenangabe
  • 11(12): 1348-1362
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2015
1000 Lizenz
1000 Verlagsversion
  • http://dx.doi.org/10.7150/ijbs.13132 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671993/ |
1000 Ergänzendes Material
  • http://www.ijbs.com/v11p1348.htm#headingA7 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Skeletal muscle is a highly metabolically active tissue that both stores and consumes energy. Important biological pathways that affect energy metabolism and metabolic fiber type in muscle cells may be identified through transcriptomic profiling of the muscle, especially ante mortem. Here, gene expression was investigated in malignant hyperthermia syndrome (MHS)-negative Duroc and Pietrian (PiNN) pigs significantly differing for the muscle fiber types slow-twitch-oxidative fiber (STO) and fast-twitch-oxidative fiber (FTO) as well as mitochondrial activity (succinate-dependent state 3 respiration rate). Longissimus muscle samples were obtained 24 h before slaughter and profiled using cDNA microarrays. Differential gene expression between Duroc and PiNN muscle samples were associated with protein ubiquitination, stem cell pluripotency, amyloid processing, and 3-phosphoinositide biosynthesis and degradation pathways. In addition, weighted gene co-expression network analysis within both breeds identified several co-expression modules that were associated with the proportion of different fiber types, mitochondrial respiratory activity, and ATP metabolism. In particular, Duroc results revealed strong correlations between mitochondrion-associated co-expression modules and STO (r = 0.78), fast-twitch glycolytic fiber (r = -0.98), complex I (r=0.72) and COX activity (r = 0.86). Other pathways in the protein-kinase-activity enriched module were positively correlated with STO (r=0.93), while negatively correlated with FTO (r = -0.72). In contrast to PiNN, co-expression modules enriched in macromolecule catabolic process, actin cytoskeleton, and transcription activator activity were associated with fiber types, mitochondrial respiratory activity, and metabolic enzyme activities. Our results highlight the importance of mitochondria for the oxidative capacity of porcine muscle and for breed-dependent molecular pathways in muscle cell fibers.
1000 Sacherschließung
lokal microarray
lokal muscle fiber
lokal muscle
lokal mitochondrial Respiratory Activity
1000 Fachgruppe
  1. Agrarwissenschaften |
  2. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/creator/TGl1LCBYdWFu|http://orcid.org/0000-0002-5707-513X|https://frl.publisso.de/adhoc/creator/VHJha29vbGp1bCwgTmFyZXM=|https://frl.publisso.de/adhoc/creator/QnJhbmQsIEJvZG8=|https://frl.publisso.de/adhoc/creator/TXVyw6FuaSwgRWR1YXJk|https://frl.publisso.de/adhoc/creator/S3Jpc2NoZWssIENhcnN0ZW4=|https://frl.publisso.de/adhoc/creator/V2lja2UsIE1pY2hhZWw=|https://frl.publisso.de/adhoc/creator/U2Nod2VyaW4sIE1hbmZyZWQ=|http://orcid.org/0000-0002-9523-6790|https://frl.publisso.de/adhoc/creator/UG9uc3Vrc2lsaSwgU2lyaWx1Y2s=
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1000 Erstellt am 2018-10-25T12:27:55.873+0200
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1000 Zuletzt bearbeitet Tue Dec 18 12:40:55 CET 2018
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