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1000 Titel
  • Ingesting a Combined Carbohydrate and Essential Amino Acid Supplement Compared to a Non-Nutritive Placebo Blunts Mitochondrial Biogenesis-Related Gene Expression after Aerobic Exercise
1000 Autor/in
  1. Margolis, Lee M |
  2. Murphy, Nancy E |
  3. Carrigan, Christopher T |
  4. McClung, Holly L |
  5. Pasiakos, Stefan M |
1000 Erscheinungsjahr 2017
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-05-23
1000 Erschienen in
1000 Quellenangabe
  • 1(6):e000893
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Whether load carriage (LC), an endurance exercise mode composed of the aerobic component of traditional endurance exercise [e.g., cycle ergometry (CE)] and contractile forces characteristic of resistive-type exercise, modulates acute mitochondrial adaptive responses to endurance exercise and supplemental nutrition [carbohydrate + essential amino acids (CHO+EAA)] is not known. OBJECTIVE: The aim of this study was to examine the effects of LC and CE, with or without CHO+EAA supplementation, on acute markers of mitochondrial biogenesis. METHODS: Twenty-five adults performed 90 min of metabolically matched LC (treadmill walking, wearing a vest equal to 30% of body mass) or CE exercise during which CHO+EAA (46 g carbohydrate and 10 g essential amino acids) or non-nutritive control (CON) drinks were consumed. Muscle biopsy samples were collected at rest (pre-exercise), post-exercise, and after 3 h of recovery to assess citrate synthase activity and the expression of mRNA (reverse transcriptase–quantitative polymerase chain reaction) and protein (Western blot). RESULTS: Citrate synthase and phosphorylated p38 mitogen-activated protein kinase (p38 MAPK)Thr180/Tyr182 were elevated postexercise compared with pre-exercise (time main effect, P < 0.05). Peroxisome proliferator-activated γ-receptor coactivator 1α (PGC-1α) expression was highest after recovery for CE compared with LC (exercise-by-time effect, P < 0.05). Sirtuin 1 (SIRT1) expression postexercise was higher for CON than for CHO+EAA treatments (drink-by-time, P < 0.05). Tumor suppressor p53 (p53), mitochondrial transcription factor A (TFAM), and cytochrome c oxidase subunit IV (COXIV) expression was greater for CON than for CHO+EAA treatments (drink main effect, P < 0.05). PGC-1α and p53 expressions were positively associated (P < 0.05) with TFAM (r = 0.629 and 0.736, respectively) and COXIV (r = 0.465 and 0.461, respectively) expressions. CONCLUSIONS: Acute mitochondrial adaptive responses to endurance exercise appear to be largely driven by exogenous nutrition availability. Although CE upregulated PGC-1α expression to a greater extent than LC, downstream signaling was the same between modes, suggesting that LC, in large part, elicits the same acute mitochondrial response as traditional, non–weight-bearing endurance exercise. This trial was registered at as NCT01714479.
1000 Sacherschließung
lokal PGC-1α
lokal SIRT1
lokal COXIV
lokal concurrent exercise
lokal TFAM
lokal p53
1000 Fächerklassifikation (DDC)
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  1. U.S. Army |
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    1000 Förderer U.S. Army |
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1000 Erstellt am 2018-12-18T13:04:09.789+0100
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