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1000 Titel
  • Enrichment of in vivo transcription data from dietary intervention studies with in vitro data provides improved insight into gene regulation mechanisms in the intestinal mucosa
1000 Autor/in
  1. Hulst, Marcel |
  2. Jansman, Alfons |
  3. Wijers, Ilonka |
  4. Hoekman, Arjan |
  5. Vastenhouw, Stéphanie |
  6. van Krimpen, Marinus |
  7. Smits, Mari |
  8. Schokker, Dirkjan |
1000 Erscheinungsjahr 2017
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2017-04-13
1000 Erschienen in
1000 Quellenangabe
  • 12:11
1000 Copyrightjahr
  • 2017
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1186/s12263-017-0559-1 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5390468/ |
1000 Ergänzendes Material
  • https://genesandnutrition.biomedcentral.com/articles/10.1186/s12263-017-0559-1#Decs |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Gene expression profiles of intestinal mucosa of chickens and pigs fed over long-term periods (days/weeks) with a diet rich in rye and a diet supplemented with zinc, respectively, or of chickens after a one-day amoxicillin treatment of chickens, were recorded recently. Such dietary interventions are frequently used to modulate animal performance or therapeutically for monogastric livestock. In this study, changes in gene expression induced by these three interventions in cultured “Intestinal Porcine Epithelial Cells” (IPEC-J2) recorded after a short-term period of 2 and 6 hours, were compared to the in vivo gene expression profiles in order to evaluate the capability of this in vitro bioassay in predicting in vivo responses. METHODS: Lists of response genes were analysed with bioinformatics programs to identify common biological pathways induced in vivo as well as in vitro. Furthermore, overlapping genes and pathways were evaluated for possible involvement in the biological processes induced in vivo by datamining and consulting literature. RESULTS: For all three interventions, only a limited number of identical genes and a few common biological processes/pathways were found to be affected by the respective interventions. However, several enterocyte-specific regulatory and secreted effector proteins that responded in vitro could be related to processes regulated in vivo, i.e. processes related to mineral absorption, (epithelial) cell adherence and tight junction formation for zinc, microtubule and cytoskeleton integrity for amoxicillin, and cell-cycle progression and mucus production for rye. CONCLUSIONS: Short-term gene expression responses to dietary interventions as measured in the in vitro bioassay have a low predictability for long-term responses as measured in the intestinal mucosa in vivo. The short-term responses of a set regulatory and effector genes, as measured in this bioassay, however, provided additional insight into how specific processes in piglets and broilers may be modulated by “early” signalling molecules produced by enterocytes. The relevance of this set of regulatory/effector genes and cognate biological processes for zinc deficiency and supplementation, gluten allergy (rye), and amoxicillin administration in humans is discussed.
1000 Sacherschließung
lokal Zinc
lokal Intestinal mucosa
lokal Gene expression
lokal Broilers
lokal Pigs
lokal Amoxicillin
lokal Rye
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. http://orcid.org/0000-0001-8020-8853|https://frl.publisso.de/adhoc/creator/SmFuc21hbiwgQWxmb25z|https://frl.publisso.de/adhoc/creator/V2lqZXJzLCBJbG9ua2E=|https://frl.publisso.de/adhoc/creator/SG9la21hbiwgQXJqYW4=|https://frl.publisso.de/adhoc/creator/VmFzdGVuaG91dywgU3TDqXBoYW5pZQ==|https://frl.publisso.de/adhoc/creator/dmFuIEtyaW1wZW4sIE1hcmludXM=|https://frl.publisso.de/adhoc/creator/U21pdHMsIE1hcmk=|https://frl.publisso.de/adhoc/creator/U2Nob2trZXIsIERpcmtqYW4=
1000 Label
1000 Förderer
  1. Ministry of Economic Affairs |
1000 Fördernummer
  1. TKI-AF12039B
1000 Förderprogramm
  1. Feed4Foodure: Voeding, darmgezondheid, en Immuniteit
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Ministry of Economic Affairs |
    1000 Förderprogramm Feed4Foodure: Voeding, darmgezondheid, en Immuniteit
    1000 Fördernummer TKI-AF12039B
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6412229.rdf
1000 Erstellt am 2019-01-24T13:10:39.157+0100
1000 Erstellt von 218
1000 beschreibt frl:6412229
1000 Bearbeitet von 218
1000 Zuletzt bearbeitet 2021-10-14T16:48:05.246+0200
1000 Objekt bearb. Thu Oct 14 16:48:04 CEST 2021
1000 Vgl. frl:6412229
1000 Oai Id
  1. oai:frl.publisso.de:frl:6412229 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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