Annamneedi2018_Article_AblationOfThePresynapticOrgani.pdf 2,66MB
1000 Titel
  • Ablation of the presynaptic organizer Bassoon in excitatory neurons retards dentate gyrus maturation and enhances learning performance
1000 Autor/in
  1. Annamneedi, Anil |
  2. Caliskan, Gürsel |
  3. Müller, Sabrina |
  4. Montag, Dirk |
  5. Budinger, Eike |
  6. Angenstein, Frank |
  7. Fejtova, Anna |
  8. Tischmeyer, Wolfgang |
  9. Gundelfinger, Eckart |
  10. Stork, Oliver |
1000 Erscheinungsjahr 2018
1000 LeibnizOpen
1000 Art der Datei
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2018-06-18
1000 Erschienen in
1000 Quellenangabe
  • 223(7):3423-3445
1000 FRL-Sammlung
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Ergänzendes Material
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Bassoon is a large scaffolding protein of the presynaptic active zone involved in the development of presynaptic terminals and in the regulation of neurotransmitter release at both excitatory and inhibitory brain synapses. Mice with constitutive ablation of the Bassoon (Bsn) gene display impaired presynaptic function, show sensory deficits and develop severe seizures. To specifically study the role of Bassoon at excitatory forebrain synapses and its relevance for control of behavior, we generated conditional knockout (Bsn cKO) mice by gene ablation through an Emx1 promoter-driven Cre recombinase. In these animals, we confirm selective loss of Bassoon from glutamatergic neurons of the forebrain. Behavioral assessment revealed that, in comparison to wild-type littermates, Bsn cKO mice display selectively enhanced contextual fear memory and increased novelty preference in a spatial discrimination/pattern separation task. These changes are accompanied by an augmentation of baseline synaptic transmission at medial perforant path to dentate gyrus (DG) synapses, as indicated by increased ratios of field excitatory postsynaptic potential slope to fiber volley amplitude. At the structural level, an increased complexity of apical dendrites of DG granule cells can be detected in Bsn cKO mice. In addition, alterations in the expression of cellular maturation markers and a lack of age-dependent decrease in excitability between juvenile and adult Bsn cKO mice are observed. Our data suggest that expression of Bassoon in excitatory forebrain neurons is required for the normal maturation of the DG and important for spatial and contextual memory.
1000 Sacherschließung
lokal Knockout mice
lokal Bassoon
lokal Immature DG
lokal Contextual fear memory
lokal Neurogenesis
lokal Spatial memory
1000 Fachgruppe
  1. Biologie |
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft
  2. Leibniz Association
1000 Fördernummer
  1. A06; B05; B09
  2. SAS-2015-LIN-LWC
1000 Förderprogramm
  1. CRC 779 “Neurobiology of Motivated Behavior”; RTG2162 “Neurodevelopment and Vulnerability of the Central Nervous System”
  2. CBBS ScienceCampus; Leibniz Graduate School “SynaptoGenetics” (Leibniz SAW program)
1000 Dateien
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6413538.rdf
1000 Erstellt am 2019-03-26T11:49:50.421+0100
1000 Erstellt von 242
1000 beschreibt frl:6413538
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Thu Jan 30 22:20:03 CET 2020
1000 Objekt bearb. Wed Mar 27 07:31:25 CET 2019
1000 Vgl. frl:6413538
1000 Oai Id
  1. |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

View source