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WeightNameValue
1000 Titel
  • Cryopreservation in rhinoceros—Setting a new benchmark for sperm cryosurvival
1000 Titelzusatz
  • Cryopreservation in rhinoceros - Setting a new benchmark for sperm cryosurvival
1000 Autor/in
  1. Hermes, Robert |
  2. Hildebrandt, Thomas Bernd |
  3. Göritz, Frank |
1000 Erscheinungsjahr 2018
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2018-07-11
1000 Erschienen in
1000 Quellenangabe
  • 13(7):e0200154
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2018
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1371/journal.pone.0200154 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6040743/ |
1000 Ergänzendes Material
  • https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0200154#sec015 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • At times when rhinoceros are fiercely poached, when some rhinoceros species are closer than ever to extinction, and when the scientific community is in debate over the use of advanced cell technologies as a remaining resort it is time to simplify and improve existing assisted reproduction techniques to enhance breeding and genetic diversity in the living populations under our care. Semen cryopreservation has been performed in all captive rhinoceros species with limited degree of success. Here we tested three freezing extenders, containing different cryoprotectants and various freezing rates for the cryopreservation of rhinoceros sperm from 14 bulls. In experiment I, semen from 9 bulls was used to determine the most suitable diluent, cryoprotectant and freezing rate for the successful cryopreservation of rhinoceros sperm. In experiment II, semen from 5 bulls was used to assess whether the removal of seminal plasma could further improve post thaw sperm quality following cryopreservation with conditions identified in Experiment I. Semen was diluted with Berliner Cryomedia, ButoCrio® or INRA Freeze®, packaged in 0.5 mL straws and frozen 3, 4, and 5 cm over liquid nitrogen (LN) vapour or directly in a dryshipper. It was found that semen extended with ButoCrio® (containing glycerol and methylformamide) and frozen 3cm over LN vapour provided the best protection to rhinoceros spermatozoa during cryopreservation. When pooled over treatments, total and progressive post thaw motility was 75.3 ± 4.2% and 68.5 ± 5.7%, respectively marking a new benchmark for the cryopreservation of rhinoceros sperm. Post thaw total and progressive motility, viability and acrosome integrity of semen diluted in ButoCrio® was significantly higher than semen extended in Berliner Cryomedia or INRA Freeze®. The removal of seminal plasma did not improve post thaw sperm survival (p > 0.05). In conclusion, the cryosurvival of rhinoceros spermatozoa was significantly improved when using a mixture of glycerol and methylformamide in combination with a fast freezing rate at 3 cm. These results describe a new protocol for the improved cryosurvival of rhinoceros spermatozoa and will enable a more successful preservation of genetic diversity between males, especially in donors whose spermatozoa may already be compromised prior to or during collection. The successful reduction of glycerol concentration in favour of methylformamide as a cryoprotectant could be a novel suggestion for the improvement of cryopreservation techniques in other wildlife species.
1000 Sacherschließung
lokal Semen
lokal Wildlife
lokal Cryopreservation
lokal Acrosomes
lokal Vapors
lokal Density gradient centrifugation
lokal Sperm
lokal Glycerolization
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0003-2487-9171|https://orcid.org/0000-0001-8685-4733|https://frl.publisso.de/adhoc/uri/R8O2cml0eiwgRnJhbms=
1000 Label
1000 Förderer
  1. Leibniz-Gemeinschaft |
1000 Fördernummer
  1. -
1000 Förderprogramm
  1. Open Access Fund
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Leibniz-Gemeinschaft |
    1000 Förderprogramm Open Access Fund
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6415002.rdf
1000 Erstellt am 2019-07-03T12:42:22.394+0200
1000 Erstellt von 218
1000 beschreibt frl:6415002
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet 2020-10-28T11:44:41.711+0100
1000 Objekt bearb. Wed Oct 28 11:44:41 CET 2020
1000 Vgl. frl:6415002
1000 Oai Id
  1. oai:frl.publisso.de:frl:6415002 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
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