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1000 Titel
  • DNA methylation analysis of porcine mammary epithelial cells reveals differentially methylated loci associated with immune response against Escherichia coli challenge
1000 Autor/in
  1. Sajjanar, Basavaraj |
  2. Trakooljul, Nares |
  3. Wimmers, Klaus |
  4. Ponsuksili, Siriluck |
1000 Erscheinungsjahr 2019
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2019-07-31
1000 Erschienen in
1000 Quellenangabe
  • 623:20
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2019
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1186/s12864-019-5976-7 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6670134/ |
1000 Ergänzendes Material
  • https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-019-5976-7#Sec19 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Epigenetic changes such as cytosine (CpG) DNA methylations regulate gene expression patterns in response to environmental cues including infections. Microbial infections induce DNA methylations that play a potential role in modulating host-immune response. In the present study, we sought to determine DNA methylation changes induced by the mastitis causing Escherichia coli (E. coli) in porcine mammary epithelial cells (PMEC). Two time points (3 h and 24 h) were selected based on specific transcriptomic changes during the early and late immune responses, respectively. RESULTS: DNA methylation analysis revealed 561 and 898 significant (P < 0.01) differentially methylated CpG sites at 3 h and 24 h after E. coli challenge in PMEC respectively. These CpG sites mapped to genes that have functional roles in innate and adaptive immune responses. Significantly, hypomethylated CpG sites were found in the promoter regions of immune response genes such as SDF4, SRXN1, CSF1 and CXCL14. The quantitative transcript estimation indicated higher expression associated with the DNA CpG methylation observed in these immune response genes. Further, E. coli challenge significantly reduced the expression levels of DNMT3a, a subtype of de novo DNA methylation enzyme, in PMEC indicating the probable reason for the hypomethylation observed in the immune response genes. CONCLUSIONS: Our study revealed E. coli infection induced DNA methylation loci in the porcine genome. The differentially methylated CpGs were identified in the regulatory regions of genes that play important role in immune response. These results will help to understand epigenetic mechanisms for immune regulation during coliform mastitis in pigs.
1000 Sacherschließung
lokal DNA methylations
lokal Immune response
lokal Coliform mastitis
lokal CpG islands
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/uri/U2FqamFuYXIsIEJhc2F2YXJhag==|https://frl.publisso.de/adhoc/uri/VHJha29vbGp1bCwgTmFyZXM=|https://orcid.org/0000-0002-9523-6790|https://frl.publisso.de/adhoc/uri/UG9uc3Vrc2lsaSwgU2lyaWx1Y2s=
1000 Label
1000 Förderer
  1. Leibniz-Institut für Nutztierbiologie |
1000 Fördernummer
  1. -
1000 Förderprogramm
  1. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Leibniz-Institut für Nutztierbiologie |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
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1000 @id frl:6416805.rdf
1000 Erstellt am 2019-10-15T15:23:04.626+0200
1000 Erstellt von 218
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1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Thu Jan 30 17:36:09 CET 2020
1000 Objekt bearb. Tue Oct 15 15:23:59 CEST 2019
1000 Vgl. frl:6416805
1000 Oai Id
  1. oai:frl.publisso.de:frl:6416805 |
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