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1000 Titel
  • The primate-specific peptide Y-P30 regulates morphological maturation of neocortical dendritic spines
1000 Autor/in
  1. Neumann, Janine R. |
  2. Dash-Wagh, Suvarna |
  3. Jack, Alexander |
  4. Räk, Andrea |
  5. Jüngling, Kay |
  6. Hamad, Mohammad I. K. |
  7. Pape, Hans-Christian |
  8. Kreutz, Michael R. |
  9. Puskarjov, Martin |
  10. Wahle, Petra |
1000 Erscheinungsjahr 2019
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2019-02-13
1000 Erschienen in
1000 Quellenangabe
  • 14(2):e0211151
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2019
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1371/journal.pone.0211151 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6373909/ |
1000 Ergänzendes Material
  • https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0211151#sec021 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The 30-amino acid peptide Y-P30 corresponds to the N-terminus of the primate-specific, sweat gland-derived dermcidin prepropeptide. Previous work has revealed that Y-P30 enhances the interaction of pleiotrophin and syndecans-2/3, and thus represents a natural ligand to study this signaling pathway. In immature neurons, Y-P30 activates the c-Src and p42/44 ERK kinase pathway, increases the amount of F-actin in axonal growth cones, and promotes neuronal survival, cell migration and axonal elongation. The action of Y-P30 on axonal growth requires syndecan-3 and heparan sulfate side chains. Whether Y-P30 has the potential to influence dendrites and dendritic protrusions has not been explored. The latter is suggested by the observations that syndecan-2 expression increases during postnatal development, that syndecan-2 becomes enriched in dendritic spines, and that overexpression of syndecan-2 in immature neurons results in a premature morphological maturation of dendritic spines. Here, analysing rat cortical pyramidal and non-pyramidal neurons in organotypic cultures, we show that Y-P30 does not alter the development of the dendritic arborization patterns. However, Y-P30 treatment decreases the density of apical, but not basal dendritic protrusions at the expense of the filopodia. Analysis of spine morphology revealed an unchanged mushroom/stubby-to-thin spine ratio and a shortening of the longest decile of dendritic protrusions. Whole-cell recordings from cortical principal neurons in dissociated cultures grown in the presence of Y-P30 demonstrated a decrease in the frequency of glutamatergic mEPSCs. Despite these differences in protrusion morphology and synaptic transmission, the latter likely attributable to presynaptic effects, calcium event rate and amplitude recorded in pyramidal neurons in organotypic cultures were not altered by Y-P30 treatment. Together, our data suggest that Y-P30 has the capacity to decelerate spinogenesis and to promote morphological, but not synaptic, maturation of dendritic protrusions.
1000 Sacherschließung
lokal Neuronal morphology
lokal Neurons
lokal Interneurons
lokal Phosphorylation
lokal Neuronal dendrites
lokal Neuronal differentiation
lokal Synapses
lokal Pyramidal cells
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/uri/TmV1bWFubiwgSmFuaW5lIFIu|https://frl.publisso.de/adhoc/uri/RGFzaC1XYWdoLCBTdXZhcm5hIA==|https://frl.publisso.de/adhoc/uri/SmFjaywgQWxleGFuZGVyIA==|https://frl.publisso.de/adhoc/uri/UsOkaywgQW5kcmVhIA==|https://frl.publisso.de/adhoc/uri/SsO8bmdsaW5nLCBLYXkg|https://frl.publisso.de/adhoc/uri/SGFtYWQsIE1vaGFtbWFkIEkuIEsuIA==|https://frl.publisso.de/adhoc/uri/UGFwZSwgSGFucy1DaHJpc3RpYW4g|https://frl.publisso.de/adhoc/uri/S3JldXR6LCBNaWNoYWVsIFIuIA==|https://orcid.org/0000-0001-7201-2615|https://orcid.org/0000-0002-8710-0375
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
  2. Bundesministerium für Bildung und Forschung |
  3. Emil Aaltosen Säätiö |
  4. Stammzellnetzerk NRW |
1000 Fördernummer
  1. -
  2. -
  3. -
  4. AZ 224 – 400 010 04
1000 Förderprogramm
  1. Graduate Program 736
  2. Förderschwerpunkt Neurotraumatologie, Teilprojekt A11
  3. -
  4. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm Graduate Program 736
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Bundesministerium für Bildung und Forschung |
    1000 Förderprogramm Förderschwerpunkt Neurotraumatologie, Teilprojekt A11
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Emil Aaltosen Säätiö |
    1000 Förderprogramm -
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer Stammzellnetzerk NRW |
    1000 Förderprogramm -
    1000 Fördernummer AZ 224 – 400 010 04
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6417208.rdf
1000 Erstellt am 2019-10-30T14:03:23.684+0100
1000 Erstellt von 242
1000 beschreibt frl:6417208
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Fri Jan 31 01:25:18 CET 2020
1000 Objekt bearb. Wed Oct 30 15:47:00 CET 2019
1000 Vgl. frl:6417208
1000 Oai Id
  1. oai:frl.publisso.de:frl:6417208 |
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