s12986-018-0277-8.pdf 1,68MB
1000 Titel
  • Novel anti-obesity effects of beer hops compound xanthohumol: role of AMPK signaling pathway
1000 Autor/in
  1. Samuels, Janaiya S. |
  2. Shashidharamurthy, Rangaiah |
  3. Rayalam, Srujana |
1000 Erscheinungsjahr 2018
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2018-06-15
1000 Erschienen in
1000 Quellenangabe
  • 15:42
1000 Copyrightjahr
  • 2018
1000 Lizenz
1000 Verlagsversion
  • |
  • |
1000 Ergänzendes Material
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Obesity alters adipose tissue metabolic and endocrine functioning, leading to an increased adiposity and release of pro-inflammatory cytokines. Various phytochemicals have been reported to contribute to the beiging of white adipose tissue in order to ameliorate obesity by increasing thermogenesis. Here, we show that the prenylated chalcone, xanthohumol (XN), induces beiging of white adipocytes, stimulates lipolysis, and inhibits adipogenesis of murine 3T3-L1 adipocytes and primary human subcutaneous preadipocytes and these effects are partly mediated by the activation of the AMP-activated protein kinase (AMPK) signaling pathway. METHODS: 3T3-L1 adipocytes and primary human subcutaneous preadipocytes were differentiated using a standard protocol and were treated with various concentrations of XN, dorsomorphin, an AMPK inhibitor, or AICAR, an AMPK activator, to investigate the effects on adipogenesis, beiging and lipolysis. RESULTS: XN induced beiging of white adipocytes as witnessed by the increased expression of beige markers CIDE-A and TBX-1. XN increased mitochondrial biogenesis, as evidenced by increased mitochondrial content, enhanced expression of PGC-1α, and the thermogenic protein UCP1. Following 24 h of treatment, XN also increased oxygen consumption rate. XN stimulated lipolysis of mature 3T3-L1 and primary human subcutaneous adipocytes and inhibited adipogenesis of maturing adipocytes. XN activated AMPK and in turn, XN-induced upregulation of UCP1, p-ACC, HSL, and ATGL was downregulated in the presence of dorsomorphin. Likewise, an XN-induced decrease in adipogenesis was reversed in the presence of dorsomorphin. CONCLUSIONS: Taken together, XN demonstrates anti-obesity effects by not only inducing beiging but also decreasing adipogenesis and inducing lipolysis. The anti-obesity effects of XN are partly mediated by AMPK signaling pathway suggesting that XN may have potential therapeutic implications for obesity.
1000 Sacherschließung
lokal ACC
lokal Obesity
lokal AMPK signaling
lokal Oxygen consumption rate
lokal 3T3-L1 cells
lokal Beiging
lokal Xanthohumol
lokal Human primary subcutaneous adipocytes
lokal Lipolysis
lokal Thermogenesis
lokal UCP1
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Label
1000 Förderer
  1. Philadelphia College of Osteopathic Medicine |
  2. National Institute of Allergy and Infectious Diseases |
1000 Fördernummer
  1. -
  2. 1R03 AI128254-01A1
1000 Förderprogramm
  1. -
  2. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Philadelphia College of Osteopathic Medicine |
    1000 Förderprogramm -
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer National Institute of Allergy and Infectious Diseases |
    1000 Förderprogramm -
    1000 Fördernummer 1R03 AI128254-01A1
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6417595.rdf
1000 Erstellt am 2019-11-19T13:38:55.988+0100
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1000 Bearbeitet von 218
1000 Zuletzt bearbeitet Tue Nov 19 13:40:18 CET 2019
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