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1000 Titel
  • Dopamine Receptor Activation Modulates the Integrity of the Perisynaptic Extracellular Matrix at Excitatory Synapses
1000 Autor/in
  1. Mitlöhner, Jessica |
  2. Kaushik, Rahul |
  3. Niekisch, Hartmut |
  4. Blondiaux, Armand |
  5. Gee, Christine |
  6. Happel, Max |
  7. Gundelfinger, Eckart |
  8. Dityatev, Alexander |
  9. Frischknecht, Renato |
  10. Seidenbecher, Constanze |
1000 Erscheinungsjahr 2020
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2020-01-21
1000 Erschienen in
1000 Quellenangabe
  • 9(2):260
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2020
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.3390/cells9020260 |
1000 Ergänzendes Material
  • https://www.mdpi.com/2073-4409/9/2/260#supplementary |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • In the brain, Hebbian-type and homeostatic forms of plasticity are affected by neuromodulators like dopamine (DA). Modifications of the perisynaptic extracellular matrix (ECM), which control the functions and mobility of synaptic receptors as well as the diffusion of transmitters and neuromodulators in the extracellular space, are crucial for the manifestation of plasticity. Mechanistic links between synaptic activation and ECM modifications are largely unknown. Here, we report that neuromodulation via D1-type DA receptors can induce targeted ECM proteolysis specifically at excitatory synapses of rat cortical neurons via proteases ADAMTS-4 and -5. We showed that receptor activation induces increased proteolysis of brevican (BC) and aggrecan, two major constituents of the adult ECM both in vivo and in vitro. ADAMTS immunoreactivity was detected near synapses, and shRNA-mediated knockdown reduced BC cleavage. We have outlined a molecular scenario of how synaptic activity and neuromodulation are linked to ECM rearrangements via increased cAMP levels, NMDA receptor activation, and intracellular calcium signaling.
1000 Sacherschließung
lokal brevican
lokal D1/D5 receptors
lokal ADAMTS 4/5
lokal NMDA receptors
lokal chondroitin sulfate proteoglycan
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0002-5125-7063|https://frl.publisso.de/adhoc/uri/S2F1c2hpaywgUmFodWw=|https://frl.publisso.de/adhoc/uri/Tmlla2lzY2gsIEhhcnRtdXQ=|https://frl.publisso.de/adhoc/uri/QmxvbmRpYXV4LCBBcm1hbmQ=|https://orcid.org/0000-0003-0345-3665|https://orcid.org/0000-0002-9581-5856|https://orcid.org/0000-0001-9377-7414|https://frl.publisso.de/adhoc/uri/RGl0eWF0ZXYsIEFsZXhhbmRlcg==|https://orcid.org/0000-0002-1268-7511|https://orcid.org/0000-0002-7433-2716
1000 Label
1000 Förderer
  1. Deutsche Forschungsgemeinschaft |
  2. H2020 Marie Skłodowska-Curie Actions |
1000 Fördernummer
  1. SFB779 TPB14; SFB779 TPB16N; SFB779 TPB09
  2. -
1000 Förderprogramm
  1. -
  2. Marie Curie ITN ECMED
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm -
    1000 Fördernummer SFB779 TPB14; SFB779 TPB16N; SFB779 TPB09
  2. 1000 joinedFunding-child
    1000 Förderer H2020 Marie Skłodowska-Curie Actions |
    1000 Förderprogramm Marie Curie ITN ECMED
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6418774.rdf
1000 Erstellt am 2020-01-31T11:26:02.710+0100
1000 Erstellt von 242
1000 beschreibt frl:6418774
1000 Bearbeitet von 16
1000 Zuletzt bearbeitet Fri Mar 13 10:06:54 CET 2020
1000 Objekt bearb. Fri Mar 13 10:06:54 CET 2020
1000 Vgl. frl:6418774
1000 Oai Id
  1. oai:frl.publisso.de:frl:6418774 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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