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WeightNameValue
1000 Titel
  • Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV
1000 Autor/in
  1. Ou, Xiuyuan |
  2. Liu, Yan |
  3. Lei, Xiaobo |
  4. Li, Pei |
  5. Mi, Dan |
  6. Ren, Lili |
  7. Guo, Li |
  8. Guo, Ruixuan |
  9. Chen, Ting |
  10. Hu, Jiaxin |
  11. Xiang, Zichun |
  12. Mu, Zhixia |
  13. Chen, Xing |
  14. Chen, Jieyong |
  15. Hu, Keping |
  16. Jin, Qi |
  17. Wang, Jianwei |
  18. Qian, Zhaohui |
1000 Erscheinungsjahr 2020
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2020-03-27
1000 Erschienen in
1000 Quellenangabe
  • 11(1):1620
1000 Copyrightjahr
  • 2020
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1038/s41467-020-15562-9 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7100515/ |
1000 Ergänzendes Material
  • https://www.nature.com/articles/s41467-020-15562-9#Sec29 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Since 2002, beta coronaviruses (CoV) have caused three zoonotic outbreaks, SARS-CoV in 2002–2003, MERS-CoV in 2012, and the newly emerged SARS-CoV-2 in late 2019. However, little is currently known about the biology of SARS-CoV-2. Here, using SARS-CoV-2 S protein pseudovirus system, we confirm that human angiotensin converting enzyme 2 (hACE2) is the receptor for SARS-CoV-2, find that SARS-CoV-2 enters 293/hACE2 cells mainly through endocytosis, that PIKfyve, TPC2, and cathepsin L are critical for entry, and that SARS-CoV-2 S protein is less stable than SARS-CoV S. Polyclonal anti-SARS S1 antibodies T62 inhibit entry of SARS-CoV S but not SARS-CoV-2 S pseudovirions. Further studies using recovered SARS and COVID-19 patients’ sera show limited cross-neutralization, suggesting that recovery from one infection might not protect against the other. Our results present potential targets for development of drugs and vaccines for SARS-CoV-2.
1000 Sacherschließung
gnd 1206347392 COVID-19
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/uri/T3UsIFhpdXl1YW4=|https://frl.publisso.de/adhoc/uri/TGl1LCBZYW4=|https://frl.publisso.de/adhoc/uri/TGVpLCBYaWFvYm8=|https://frl.publisso.de/adhoc/uri/TGksIFBlaQ==|https://frl.publisso.de/adhoc/uri/TWksIERhbg==|https://frl.publisso.de/adhoc/uri/UmVuLCBMaWxp|https://frl.publisso.de/adhoc/uri/R3VvLCBMaQ==|https://frl.publisso.de/adhoc/uri/R3VvLCBSdWl4dWFu|https://frl.publisso.de/adhoc/uri/Q2hlbiwgVGluZw==|https://frl.publisso.de/adhoc/uri/SHUsIEppYXhpbg==|https://frl.publisso.de/adhoc/uri/WGlhbmcsIFppY2h1bg==|https://frl.publisso.de/adhoc/uri/TXUsIFpoaXhpYQ==|https://frl.publisso.de/adhoc/uri/Q2hlbiwgWGluZw==|https://frl.publisso.de/adhoc/uri/Q2hlbiwgSmlleW9uZw==|https://frl.publisso.de/adhoc/uri/SHUsIEtlcGluZw==|https://frl.publisso.de/adhoc/uri/SmluLCBRaQ==|https://orcid.org/0000-0002-1116-4559|https://orcid.org/0000-0003-4116-4450
1000 Label
1000 Förderer
  1. Ministry of Science and Technology, Government of the People’s Republic of Bangladesh |
  2. National Natural Science Foundation of China |
  3. Chinese Academy of Medical Sciences |
1000 Fördernummer
  1. 2020YFC0841000; 2017ZX0103004
  2. 31670164; 31970171; 81930063
  3. 2020HY320001; 2016-12M-1-014
1000 Förderprogramm
  1. Science and Technology Key Projects
  2. -
  3. COVID-19 Emergency Fund; Innovation Fund for Medical Sciences
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Ministry of Science and Technology, Government of the People’s Republic of Bangladesh |
    1000 Förderprogramm Science and Technology Key Projects
    1000 Fördernummer 2020YFC0841000; 2017ZX0103004
  2. 1000 joinedFunding-child
    1000 Förderer National Natural Science Foundation of China |
    1000 Förderprogramm -
    1000 Fördernummer 31670164; 31970171; 81930063
  3. 1000 joinedFunding-child
    1000 Förderer Chinese Academy of Medical Sciences |
    1000 Förderprogramm COVID-19 Emergency Fund; Innovation Fund for Medical Sciences
    1000 Fördernummer 2020HY320001; 2016-12M-1-014
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6419634.rdf
1000 Erstellt am 2020-04-01T15:06:21.155+0200
1000 Erstellt von 122
1000 beschreibt frl:6419634
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet Wed Apr 01 15:23:39 CEST 2020
1000 Objekt bearb. Wed Apr 01 15:23:39 CEST 2020
1000 Vgl. frl:6419634
1000 Oai Id
  1. oai:frl.publisso.de:frl:6419634 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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