Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

  1. Corman, Victor M.
  2. Landt, Olfert
  3. Kaiser, Marco
  4. Molenkamp, Richard
  5. Meijer, Adam
  6. Chu, Daniel K. W.
  7. Bleicker, Tobias
  8. Brünink, Sebastian
  9. Schneider, Julia
  10. Schmidt, Marie Luisa
  11. Mulders, Daphne G. J. C.
  12. Haagmans, Bart L.
  13. van der Veer, Bas
  14. van den Brink, Sharon
  15. Wijsman, Lisa
  16. Goderski, Gabriel
  17. Romette, Jean-Louis
  18. Ellis, Joanna
  19. Zambon, Maria
  20. Peiris, Malik
  21. Goossens, Herman
  22. Reusken, Chantal
  23. Koopmans, Marion P. G.
  24. Drosten, Christian ORCID logo

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WeightNameValue
1000 Titel
  • Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
1000 Autor/in
  1. Corman, Victor M. |
  2. Landt, Olfert |
  3. Kaiser, Marco |
  4. Molenkamp, Richard |
  5. Meijer, Adam |
  6. Chu, Daniel K. W. |
  7. Bleicker, Tobias |
  8. Brünink, Sebastian |
  9. Schneider, Julia |
  10. Schmidt, Marie Luisa |
  11. Mulders, Daphne G. J. C. |
  12. Haagmans, Bart L. |
  13. van der Veer, Bas |
  14. van den Brink, Sharon |
  15. Wijsman, Lisa |
  16. Goderski, Gabriel |
  17. Romette, Jean-Louis |
  18. Ellis, Joanna |
  19. Zambon, Maria |
  20. Peiris, Malik |
  21. Goossens, Herman |
  22. Reusken, Chantal |
  23. Koopmans, Marion P. G. |
  24. Drosten, Christian |
1000 Erscheinungsjahr 2020
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2020-01-23
1000 Erschienen in
1000 Quellenangabe
  • 25(3):2000045
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2020
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988269/ |
1000 Ergänzendes Material
  • https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2020.25.3.2000045#supplementary_data |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur. AIM: We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available. METHODS: Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology. RESULTS: The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive – Global (EVAg), a European Union infrastructure project. CONCLUSION: The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks.
1000 Sacherschließung
gnd 1206347392 COVID-19
lokal RT-PCR
lokal Wuhan
lokal novel coronavirus
lokal 2019-nCoV
lokal diagnostics
lokal laboratory
lokal testing
lokal Coronavirus
lokal outbreak
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/uri/Q29ybWFuLCBWaWN0b3IgTS4=|https://frl.publisso.de/adhoc/uri/TGFuZHQsIE9sZmVydA==|https://frl.publisso.de/adhoc/uri/S2Fpc2VyLCBNYXJjbw==|https://frl.publisso.de/adhoc/uri/TW9sZW5rYW1wLCBSaWNoYXJk|https://frl.publisso.de/adhoc/uri/TWVpamVyLCBBZGFt|https://frl.publisso.de/adhoc/uri/Q2h1LCBEYW5pZWwgSy4gVy4=|https://frl.publisso.de/adhoc/uri/QmxlaWNrZXIsIFRvYmlhcw==|https://frl.publisso.de/adhoc/uri/QnLDvG5pbmssIFNlYmFzdGlhbg==|https://frl.publisso.de/adhoc/uri/U2NobmVpZGVyLCBKdWxpYQ==|https://frl.publisso.de/adhoc/uri/U2NobWlkdCwgTWFyaWUgTHVpc2E=|https://frl.publisso.de/adhoc/uri/TXVsZGVycywgRGFwaG5lIEcuIEouIEMu|https://frl.publisso.de/adhoc/uri/SGFhZ21hbnMsIEJhcnQgTC4=|https://frl.publisso.de/adhoc/uri/dmFuIGRlciBWZWVyLCBCYXM=|https://frl.publisso.de/adhoc/uri/dmFuIGRlbiBCcmluaywgU2hhcm9u|https://frl.publisso.de/adhoc/uri/V2lqc21hbiwgTGlzYQ==|https://frl.publisso.de/adhoc/uri/R29kZXJza2ksIEdhYnJpZWw=|https://frl.publisso.de/adhoc/uri/Um9tZXR0ZSwgSmVhbi1Mb3Vpcw==|https://frl.publisso.de/adhoc/uri/RWxsaXMsIEpvYW5uYQ==|https://frl.publisso.de/adhoc/uri/WmFtYm9uLCBNYXJpYQ==|https://frl.publisso.de/adhoc/uri/UGVpcmlzLCBNYWxpaw==|https://frl.publisso.de/adhoc/uri/R29vc3NlbnMsIEhlcm1hbg==|https://frl.publisso.de/adhoc/uri/UmV1c2tlbiwgQ2hhbnRhbA==|https://frl.publisso.de/adhoc/uri/S29vcG1hbnMsIE1hcmlvbiBQLiBHLg==|https://orcid.org/0000-0001-7923-0519
1000 Label
1000 Förderer
  1. European Union |
  2. Bundesministerium für Bildung und Forschung |
1000 Fördernummer
  1. GA602525; GA643476; GA653316
  2. 01KI1723A; 301-4-7-01.703
1000 Förderprogramm
  1. Prepare; Compare; EVAg
  2. RAPID; DZIF
1000 Dateien
  1. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
    Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer European Union |
    1000 Förderprogramm Prepare; Compare; EVAg
    1000 Fördernummer GA602525; GA643476; GA653316
  2. 1000 joinedFunding-child
    1000 Förderer Bundesministerium für Bildung und Forschung |
    1000 Förderprogramm RAPID; DZIF
    1000 Fördernummer 01KI1723A; 301-4-7-01.703
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6419818.rdf
1000 Erstellt am 2020-04-07T12:26:22.614+0200
1000 Erstellt von 25
1000 beschreibt frl:6419818
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Wed Apr 08 09:01:52 CEST 2020
1000 Objekt bearb. Wed Apr 08 09:01:52 CEST 2020
1000 Vgl. frl:6419818
1000 Oai Id
  1. oai:frl.publisso.de:frl:6419818 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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