Download
genes-11-00296-v2.pdf 1,44MB
WeightNameValue
1000 Titel
  • Standards for Methods Utilizing Environmental DNA for Detection of Fish Species
1000 Autor/in
  1. Shu, Lu |
  2. Ludwig, Arne |
  3. Peng, Zuogang |
1000 Erscheinungsjahr 2020
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2020-03-11
1000 Erschienen in
1000 Quellenangabe
  • 11(3):296
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2020
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.3390/genes11030296 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140814/ |
1000 Ergänzendes Material
  • https://www.mdpi.com/2073-4425/11/3/296#supplementary |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Environmental DNA (eDNA) techniques are gaining attention as cost-effective, non-invasive strategies for acquiring information on fish and other aquatic organisms from water samples. Currently, eDNA approaches are used to detect specific fish species and determine fish community diversity. Various protocols used with eDNA methods for aquatic organism detection have been reported in different eDNA studies, but there are no general recommendations for fish detection. Herein, we reviewed 168 papers to supplement and highlight the key criteria for each step of eDNA technology in fish detection and provide general suggestions for eliminating detection errors. Although there is no unified recommendation for the application of diverse eDNA in detecting fish species, in most cases, 1 or 2 L surface water collection and eDNA capture on 0.7-μm glass fiber filters followed by extraction with a DNeasy Blood and Tissue Kit or PowerWater DNA Isolation Kit are useful for obtaining high-quality eDNA. Subsequently, species-specific quantitative polymerase chain reaction (qPCR) assays based on mitochondrial cytochrome b gene markers or eDNA metabarcoding based on both 12S and 16S rRNA markers via high-throughput sequencing can effectively detect target DNA or estimate species richness. Furthermore, detection errors can be minimized by mitigating contamination, negative control, PCR replication, and using multiple genetic markers. Our aim is to provide a useful strategy for fish eDNA technology that can be applied by researchers, advisors, and managers.
1000 Sacherschließung
lokal detection error
lokal genetic marker
lokal eDNA capture
lokal environmental DNA
lokal water sampling
lokal eDNA detection
lokal eDNA extraction
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/uri/U2h1LCBMdQ==|https://orcid.org/0000-0001-7249-9953|https://orcid.org/0000-0001-8810-2025
1000 Label
1000 Förderer
  1. National Key Research and Development Program of China |
  2. National Natural Science Foundation of China |
1000 Fördernummer
  1. 2018YFD0900805
  2. -
1000 Förderprogramm
  1. -
  2. 31872204
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer National Key Research and Development Program of China |
    1000 Förderprogramm -
    1000 Fördernummer 2018YFD0900805
  2. 1000 joinedFunding-child
    1000 Förderer National Natural Science Foundation of China |
    1000 Förderprogramm 31872204
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6426919.rdf
1000 Erstellt am 2021-04-19T11:55:16.095+0200
1000 Erstellt von 122
1000 beschreibt frl:6426919
1000 Bearbeitet von 122
1000 Zuletzt bearbeitet Mon Apr 19 11:57:58 CEST 2021
1000 Objekt bearb. Mon Apr 19 11:56:28 CEST 2021
1000 Vgl. frl:6426919
1000 Oai Id
  1. oai:frl.publisso.de:frl:6426919 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

View source