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1000 Titel
  • DNA Metabarcoding for the Characterization of Terrestrial Microbiota—Pitfalls and Solutions
1000 Autor/in
  1. Francioli, Davide |
  2. Lentendu, Guillaume |
  3. Lewin, Simon |
  4. Kolb, Steffen |
1000 Erscheinungsjahr 2021
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-02-12
1000 Erschienen in
1000 Quellenangabe
  • 9(2):361
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.3390/microorganisms9020361 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Soil-borne microbes are major ecological players in terrestrial environments since they cycle organic matter, channel nutrients across trophic levels and influence plant growth and health. Therefore, the identification, taxonomic characterization and determination of the ecological role of members of soil microbial communities have become major topics of interest. The development and continuous improvement of high-throughput sequencing platforms have further stimulated the study of complex microbiota in soils and plants. The most frequently used approach to study microbiota composition, diversity and dynamics is polymerase chain reaction (PCR), amplifying specific taxonomically informative gene markers with the subsequent sequencing of the amplicons. This methodological approach is called DNA metabarcoding. Over the last decade, DNA metabarcoding has rapidly emerged as a powerful and cost-effective method for the description of microbiota in environmental samples. However, this approach involves several processing steps, each of which might introduce significant biases that can considerably compromise the reliability of the metabarcoding output. The aim of this review is to provide state-of-the-art background knowledge needed to make appropriate decisions at each step of a DNA metabarcoding workflow, highlighting crucial steps that, if considered, ensures an accurate and standardized characterization of microbiota in environmental studies.
1000 Sacherschließung
lokal fungi
lokal bacteria
lokal terrestrial ecosystem
lokal protists
lokal archaea
lokal soil and plant-associated microorganisms
lokal DNA metabarcoding workflow
lokal high-throughput sequencing
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0001-6572-3574|https://orcid.org/0000-0003-3826-6253|https://frl.publisso.de/adhoc/uri/TGV3aW4sIFNpbW9u|https://orcid.org/0000-0002-5455-8662
1000 (Academic) Editor
1000 Label
1000 Förderer
  1. Leibniz-Gemeinschaft |
1000 Fördernummer
  1. K102/2018
1000 Förderprogramm
  1. Volcorn-Volatilome of a Cereal Crop Microbiota Complex under drought and Flooding
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Leibniz-Gemeinschaft |
    1000 Förderprogramm Volcorn-Volatilome of a Cereal Crop Microbiota Complex under drought and Flooding
    1000 Fördernummer K102/2018
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6430175.rdf
1000 Erstellt am 2021-11-10T13:17:44.534+0100
1000 Erstellt von 317
1000 beschreibt frl:6430175
1000 Bearbeitet von 317
1000 Zuletzt bearbeitet Wed Nov 10 13:18:45 CET 2021
1000 Objekt bearb. Wed Nov 10 13:18:32 CET 2021
1000 Vgl. frl:6430175
1000 Oai Id
  1. oai:frl.publisso.de:frl:6430175 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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