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1000 Titel
  • An Evaluation of Hepatitis E Virus Molecular Typing Methods
1000 Autor/in
  1. Baylis, Sally |
  2. Adlhoch, Cornelia |
  3. Childs, Liam |
1000 Mitwirkende/r
  1. HEV Sequencing Study Group |
1000 Erscheinungsjahr 2021
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-12-30
1000 Erschienen in
1000 Quellenangabe
  • 68(1):181-191
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1093/clinchem/hvab186 |
1000 Ergänzendes Material
  • https://academic.oup.com/clinchem/article/68/1/181/6490235?login=false#supplementary-data |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Hepatitis E virus (HEV) is a major cause of acute viral hepatitis. Better understanding of HEV subtypes involved in hepatitis E infections is essential. Investigation of sources and routes of transmission and the identification of potential clusters/outbreaks rely upon molecular typing of viral strains. A study was carried out to evaluate the ability of laboratories to undertake molecular typing with genotype and subtype determination. METHODS: A blinded panel of 11 different Orthohepevirus A strains was distributed to 28 laboratories performing HEV sequence analysis. Laboratories used their routine HEV sequencing and genotyping methods. RESULTS: Results were returned by 25 laboratories. Overall, 93% samples were assigned to the correct genotype and 81% were assigned to the correct subtype. Fragments amplified for typing ranged in size and the sequencing assays targeted both the structural and non-structural protein-coding regions. There was good agreement between the reported sequences where methods targeted overlapping fragments. In some cases, incorrect genotypes/subtypes were reported, including those not contained in the panel, and in one case, a genotype was reported for a blinded control sample containing Zika virus; collectively these data indicate contamination problems. CONCLUSIONS: In general, identification of genotypes was good; however, in a small number of cases, there was a failure to generate sequences from some of the samples. There was generally broad agreement between the use of online typing tools such as the one provided by HEVnet and curated lists of published HEV reference sequences; however, going forward harmonization between these resources is essential.
1000 Sacherschließung
lokal genotype
lokal subtype
gnd 1042401683 Hepatitis E
lokal HEV
lokal sequencing
lokal hepatitis E virus
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0002-2841-2918|https://orcid.org/0000-0002-8842-0469|https://orcid.org/0000-0002-2080-1804|https://frl.publisso.de/adhoc/uri/SEVWIFNlcXVlbmNpbmcgU3R1ZHkgR3JvdXA=
1000 Label
1000 Förderer
  1. European Centre for Disease Prevention and Control |
  2. Health Canada |
  3. Bundesministerium für Gesundheit |
  4. Ministero della Salute |
  5. Netherlands Food and Consumer Product Safety Authority (NVWA) |
  6. Ministerio de Ciencia, Innovación y Universidades |
  7. European Centre for Disease Prevention and Control |
1000 Fördernummer
  1. ECD.9621
  2. -
  3. ZMVI1-2518FSB705
  4. -
  5. -
  6. AGL2017-82909
  7. -
1000 Förderprogramm
  1. service contract
  2. internal funds
  3. CHED project grant
  4. -
  5. -
  6. -
  7. -
1000 Dateien
  1. An Evaluation of Hepatitis E Virus Molecular Typing Methods
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer European Centre for Disease Prevention and Control |
    1000 Förderprogramm service contract
    1000 Fördernummer ECD.9621
  2. 1000 joinedFunding-child
    1000 Förderer Health Canada |
    1000 Förderprogramm internal funds
    1000 Fördernummer -
  3. 1000 joinedFunding-child
    1000 Förderer Bundesministerium für Gesundheit |
    1000 Förderprogramm CHED project grant
    1000 Fördernummer ZMVI1-2518FSB705
  4. 1000 joinedFunding-child
    1000 Förderer Ministero della Salute |
    1000 Förderprogramm -
    1000 Fördernummer -
  5. 1000 joinedFunding-child
    1000 Förderer Netherlands Food and Consumer Product Safety Authority (NVWA) |
    1000 Förderprogramm -
    1000 Fördernummer -
  6. 1000 joinedFunding-child
    1000 Förderer Ministerio de Ciencia, Innovación y Universidades |
    1000 Förderprogramm -
    1000 Fördernummer AGL2017-82909
  7. 1000 joinedFunding-child
    1000 Förderer European Centre for Disease Prevention and Control |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6431676.rdf
1000 Erstellt am 2022-02-18T10:13:38.518+0100
1000 Erstellt von 323
1000 beschreibt frl:6431676
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet 2022-02-24T10:20:13.286+0100
1000 Objekt bearb. Thu Feb 24 10:19:37 CET 2022
1000 Vgl. frl:6431676
1000 Oai Id
  1. oai:frl.publisso.de:frl:6431676 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

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