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1000 Titel
  • Human monocyte-derived type 1 and 2 macrophages recognize Ara h 1, a major peanut allergen, by different mechanisms
1000 Autor/in
  1. Krause, Maren |
  2. Crauwels, Peter |
  3. Blanco Pérez, Frank Eliezer |
  4. Globisch, Martin |
  5. Wangorsch, Andrea |
  6. Henle, Thomas |
  7. Lidholm, Jonas |
  8. van Zandbergen, Ger |
  9. Vieths, Stefan |
  10. scheurer, stephan |
  11. Toda, Masako |
1000 Erscheinungsjahr 2021
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-05-12
1000 Erschienen in
1000 Quellenangabe
  • 11:10141
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1038/s41598-021-89402-1 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115286/ |
1000 Ergänzendes Material
  • https://www.nature.com/articles/s41598-021-89402-1#Sec16 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Evidence has suggested that major peanut allergen Ara h 1 activates dendritic cells (DCs) via interaction with DC-SIGN (dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin), a C-type lectin receptor, and contributes to development of peanut allergy. Since macrophages, as well as DCs, play a crucial role in innate immunity, we investigated whether natural Ara h 1 (nAra h 1) activates two different subsets of macrophages, human monocyte derived macrophage type 1 (hMDM1: pro-inflammatory model) and type 2 (hMDM2: anti-inflammatory model). hMDM1 and hMDM2 predominantly produced pro-inflammatory cytokines (IL-6 and TNF-α) and an anti-inflammatory cytokine (IL-10) in response to nAra h 1, respectively. hMDM2 took up nAra h 1 and expressed DC-SIGN at higher levels than hMDM1. However, small interfering RNA knockdown of DC-SIGN did not suppress nAra h 1 uptake and nAra h 1-mediated cytokine production in hMDM2. Inhibitors of scavenger receptor class A type I (SR-AI) suppressed the response of hMDM2, but not of hMDM1, suggesting that SR-AI is a major receptor in hMDM2 for nAra h 1 recognition and internalization. nAra h 1 appears to exert stimulatory capacity on DC and macrophages via different receptors. This study advances our understanding how a major peanut allergen interacts with innate immunity.
1000 Sacherschließung
lokal Immunology
lokal Molecular biology
gnd 4034871-4 Lebensmittelallergie
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0003-4407-336X|https://frl.publisso.de/adhoc/uri/Q3JhdXdlbHMsIFBldGVyIA==|https://orcid.org/0000-0003-3092-539X|https://frl.publisso.de/adhoc/uri/R2xvYmlzY2gsIE1hcnRpbg==|https://orcid.org/0000-0002-5184-5455|https://frl.publisso.de/adhoc/uri/SGVubGUsIFRob21hcw==|https://frl.publisso.de/adhoc/uri/IExpZGhvbG0sIEpvbmFz|https://orcid.org/0000-0001-5218-4962|https://d-nb.info/gnd/143949705|https://orcid.org/0000-0002-2859-562X|https://orcid.org/0000-0002-7273-9977
1000 Label
1000 Förderer
  1. Japan Society for the Promotion of Science |
  2. Projekt DEAL |
1000 Fördernummer
  1. 18H05998
  2. -
1000 Förderprogramm
  1. KAKENHI ; Core-to-Core Program Advanced Research Networks entitled “Establishment of international agricultural immunology research-core for a quantum improvement in food safety”
  2. Open Access funding
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Japan Society for the Promotion of Science |
    1000 Förderprogramm KAKENHI ; Core-to-Core Program Advanced Research Networks entitled “Establishment of international agricultural immunology research-core for a quantum improvement in food safety”
    1000 Fördernummer 18H05998
  2. 1000 joinedFunding-child
    1000 Förderer Projekt DEAL |
    1000 Förderprogramm Open Access funding
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6432007.rdf
1000 Erstellt am 2022-03-07T13:08:36.567+0100
1000 Erstellt von 323
1000 beschreibt frl:6432007
1000 Bearbeitet von 25
1000 Zuletzt bearbeitet Fri Mar 18 07:25:38 CET 2022
1000 Objekt bearb. Fri Mar 18 07:20:27 CET 2022
1000 Vgl. frl:6432007
1000 Oai Id
  1. oai:frl.publisso.de:frl:6432007 |
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1000 Sichtbarkeit Daten public
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