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1000 Titel
  • Contemporary Transposon Tools: A Review and Guide through Mechanisms and Applications of Sleeping Beauty, piggyBac and Tol2 for Genome Engineering
1000 Autor/in
  1. Sandoval-Villegas, Nicolás |
  2. Nurieva, Wasifa |
  3. Amberger, Maximilian |
  4. Ivics, Zoltan |
1000 Erscheinungsjahr 2021
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-05-11
1000 Erschienen in
1000 Quellenangabe
  • 22(10):5084
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2021
1000 Lizenz
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1000 Abstract/Summary
  • Transposons are mobile genetic elements evolved to execute highly efficient integration of their genes into the genomes of their host cells. These natural DNA transfer vehicles have been harnessed as experimental tools for stably introducing a wide variety of foreign DNA sequences, including selectable marker genes, reporters, shRNA expression cassettes, mutagenic gene trap cassettes, and therapeutic gene constructs into the genomes of target cells in a regulated and highly efficient manner. Given that transposon components are typically supplied as naked nucleic acids (DNA and RNA) or recombinant protein, their use is simple, safe, and economically competitive. Thus, transposons enable several avenues for genome manipulations in vertebrates, including transgenesis for the generation of transgenic cells in tissue culture comprising the generation of pluripotent stem cells, the production of germline-transgenic animals for basic and applied research, forward genetic screens for functional gene annotation in model species and therapy of genetic disorders in humans. This review describes the molecular mechanisms involved in transposition reactions of the three most widely used transposon systems currently available (Sleeping Beauty, piggyBac, and Tol2), and discusses the various parameters and considerations pertinent to their experimental use, highlighting the state-of-the-art in transposon technology in diverse genetic applications.
1000 Sacherschließung
lokal genome engineering
lokal transposition
lokal induced pluripotent stem cells
lokal transgenesis
lokal transposon
lokal genetic screens
gnd 4233582-6 Transposon
lokal nonviral
lokal gene therapy
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