Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas

  1. Wangorsch, Andrea ORCID logo
  2. Jamin, Annette
  3. Eichhorn, Stephanie
  4. Pablos, Isabel
  5. Sharma, Swati
  6. Schweidler, Bettina
  7. Kastner, Bianca
  8. Wildner, Sabrina ORCID logo
  9. Saloga, Joachim
  10. Führer, Frank
  11. Reyna Orozco, Reinaldo Rafael
  12. Sherkat, Roya
  13. Sadeghi, Somayeh
  14. Teifoori, Fardis ORCID logo
  15. Park, Jung-Won
  16. Briza, Peter ORCID logo
  17. Vieths, Stefan
  18. Ferreira, Fatima
  19. Arora, Naveen
  20. Lidholm, Jonas ORCID logo
  21. Gadermaier, Gabriele ORCID logo
  22. scheurer, stephan ORCID logo

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1000 Titel
  • Component-Resolved Diagnosis of American Cockroach (Periplaneta americana) Allergy in Patients From Different Geographical Areas
1000 Autor/in
  1. Wangorsch, Andrea |
  2. Jamin, Annette |
  3. Eichhorn, Stephanie |
  4. Pablos, Isabel |
  5. Sharma, Swati |
  6. Schweidler, Bettina |
  7. Kastner, Bianca |
  8. Wildner, Sabrina |
  9. Saloga, Joachim |
  10. Führer, Frank |
  11. Reyna Orozco, Reinaldo Rafael |
  12. Sherkat, Roya |
  13. Sadeghi, Somayeh |
  14. Teifoori, Fardis |
  15. Park, Jung-Won |
  16. Briza, Peter |
  17. Vieths, Stefan |
  18. Ferreira, Fatima |
  19. Arora, Naveen |
  20. Lidholm, Jonas |
  21. Gadermaier, Gabriele |
  22. scheurer, stephan |
1000 Erscheinungsjahr 2021
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-06-29
1000 Erschienen in
1000 Quellenangabe
  • 2:691627
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.3389/falgy.2021.691627 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8974670/ |
1000 Ergänzendes Material
  • https://www.frontiersin.org/articles/10.3389/falgy.2021.691627/full#h11 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: Manifestation of respiratory allergy to American cockroach (Periplaneta americana) is prominent in the subtropical and tropical areas. However, co-existing perennial indoor inhalant allergies frequently compromise clinical diagnosis of cockroach allergy, and the analysis of sensitization pattern is limited by the lack of Periplaneta allergens widely available for component-resolved diagnostics (CRD). OBJECTIVE: To evaluate a collection of previously described recombinant Periplaneta allergens for CRD in cockroach allergy. METHODS: A panel of nine recombinant Periplaneta allergens (Per a 1–5, 7–10) was generated, purified, and subjected to physicochemical characterization by applying circular dichroism (CD) spectroscopy, dynamic light scattering (DLS), amino acid (AA) analysis, and mass spectrometry (MS). Patients (n = 117) from India, Korea, Venezuela, and Iran, reporting perennial respiratory indoor allergies with IgE sensitization to cockroach (P. americana and/or Blattella germanica), were included. The sensitization profile was monitored by the experimental ImmunoCAP testing. RESULTS: ImmunoCAP testing confirmed IgE sensitization to Periplaneta and/or Blattella extract in 98 of 117 patients (r = 0.95). Five out of 117 patients were sensitized to only one of the two cockroach species. Within the whole study group, the prevalence of sensitization to individual allergens varied from 4% (Per a 2) to 50% (Per a 9), with the highest IgE values to Per a 9. Patients from four countries displayed different sensitization profiles at which Per a 3 and Per a 9 were identified as major allergens in India and Korea. Periplaneta-derived lipocalin and myosin light chain were characterized as new minor allergens, designated as Per a 4 and Per a 8. Periplaneta extract showed higher diagnostic sensitivity than all individual components combined, suggesting the existence of allergens yet to be discovered. CONCLUSION: Utilization of a panel of purified Periplaneta allergens revealed highly heterogeneous sensitization patterns and allowed the classification of lipocalin and myosin light chain from Periplaneta as new minor allergens.
1000 Sacherschließung
lokal American cockroach
lokal Blattella germanica
lokal component-resolved diagnosis
lokal cockroach allergy
lokal Periplaneta americana (Insecta)
gnd 4197432-3 Insektenallergie
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0002-5184-5455|https://frl.publisso.de/adhoc/uri/ICAgIEphbWluLCBBbm5ldHRlICAgICAgICA=|https://frl.publisso.de/adhoc/uri/IEVpY2hob3JuLCBTdGVwaGFuaWUgICAgICAg|https://frl.publisso.de/adhoc/uri/UGFibG9zLCBJc2FiZWwg|https://frl.publisso.de/adhoc/uri/IFNoYXJtYSwgU3dhdGkgICAgICA=|https://frl.publisso.de/adhoc/uri/ICBTY2h3ZWlkbGVyLCBCZXR0aW5hICAgICAgIA==|https://frl.publisso.de/adhoc/uri/ICBLYXN0bmVyLCBCaWFuY2EgICAgIA==|https://orcid.org/0000-0003-2348-7598|https://frl.publisso.de/adhoc/uri/ICAgIFNhbG9nYSwgSm9hY2hpbSAgICAgICA=|https://d-nb.info/gnd/133681807|https://frl.publisso.de/adhoc/uri/ICAgUmV5bmEgT3JvemNvLCBSZWluYWxkbyBSYWZhZWwgICAgICAg|https://frl.publisso.de/adhoc/uri/ICBTaGVya2F0LCBSb3lhICAgICA=|https://frl.publisso.de/adhoc/uri/ICAgIFNhZGVnaGksIFNvbWF5ZWggICAgIA==|https://orcid.org/0000-0002-7613-0325|https://frl.publisso.de/adhoc/uri/ICBQYXJrLCBKdW5nLVdvbiAg|https://orcid.org/0000-0002-4941-6782|https://d-nb.info/gnd/143949705|https://frl.publisso.de/adhoc/uri/RmVycmVpcmEsIEZhdGltYSA=|https://frl.publisso.de/adhoc/uri/ICBBcm9yYSwgTmF2ZWVuICAgIA==|https://orcid.org/0000-0002-7779-3411|https://orcid.org/0000-0002-4886-417X|https://orcid.org/0000-0002-2859-562X
1000 (Academic) Editor
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  1. ERAnet New INDIGO Project GENALL |
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  1. DBT2-038
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1000 Erstellt am 2022-03-17T10:22:40.549+0100
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1000 Zuletzt bearbeitet Thu Apr 07 11:02:56 CEST 2022
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  1. oai:frl.publisso.de:frl:6432324 |
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