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1000 Titel
  • Persistence of infectious SARS-CoV-2 particles for up to 37 days in patients with mild COVID-19
1000 Autor/in
  1. Zahn, Tobias |
  2. Mhedhbi, Ines |
  3. Hein, Sascha |
  4. Raupach, Jan |
  5. Miskey, Csaba |
  6. Husria, Younes |
  7. Bayanga, Kathrin |
  8. Bartel, Detlef |
  9. Vieths, Stefan |
  10. Ivics, Zoltan |
  11. Oberle, Doris Franziska |
  12. Keller-Stanislawski, Brigitte |
  13. Herrlein, Marie-Luise |
  14. Maier, Thorsten Jürgen |
  15. hildt, eberhard |
1000 Erscheinungsjahr 2021
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-10-12
1000 Erschienen in
1000 Quellenangabe
  • Early View
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1111/all.15138 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8652783/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: People suffering from COVID-19 are typically considered non-infectious 14 days after diagnosis if symptoms have disappeared for at least 48 h. We describe three patients who independently acquired their infection. These three patients experienced mild COVID-19 and completely recovered symptomatically within 10 days, but remained PCR-positive in deep pharyngeal samples for at least 38 days. We attempted to isolate virus from pharyngeal swabs to investigate whether these patients still carried infectious virus. METHODS: Infectious virus was amplified in Vero E6 cells and characterized by electron microscopy and WGS. The immune response was investigated by ELISA and peptide arrays. RESULTS: In all three cases, infectious and replication-competent virus was isolated and amplified in Vero E6 cells. Virus replication was detected by RT-PCR and immunofluorescence microscopy. Electron microscopy confirmed the formation of intact SARS-CoV-2 particles. For a more detailed analysis, all three isolates were characterized by whole-genome sequencing (WGS). The sequence data revealed that the isolates belonged to the 20A or 20C clade, and two mutations in ORF8 were identified among other mutations that could be relevant for establishing a long-term infection. Characterization of the humoral immune response in comparison to patients that had fully recovered from mild COVID-19 revealed a lack of antibodies binding to sequential epitopes of the receptor-binding domain (RBD) for the long-term infected patients. CONCLUSION: Thus, a small portion of COVID-19 patients displays long-term infectivity and termination of quarantine periods after 14 days, without PCR-based testing, should be reconsidered critically.
1000 Sacherschließung
gnd 1206347392 COVID-19
lokal mutations
lokal whole-genome sequencing
lokal SARS-CoV-2
lokal SARS-CoV2
lokal persistence
lokal antibodies
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://d-nb.info/gnd/1216847819|https://orcid.org/0000-0002-1165-4355|https://orcid.org/0000-0001-7257-4538|https://frl.publisso.de/adhoc/uri/UmF1cGFjaCwgSmFu|https://orcid.org/0000-0002-7566-9556|https://frl.publisso.de/adhoc/uri/ICAgICBIdXNyaWEsIFlvdW5lcyAgICAgICAg|https://frl.publisso.de/adhoc/uri/QmF5YW5nYSwgS2F0aHJpbiAgICAgICA=|https://frl.publisso.de/adhoc/uri/IEJhcnRlbCwgRGV0bGVmICAgICAg|https://d-nb.info/gnd/143949705|https://orcid.org/0000-0002-7803-6658|https://d-nb.info/gnd/141799676|https://d-nb.info/gnd/1157276989|https://orcid.org/0000-0003-2466-5114|https://orcid.org/0000-0003-3234-947X|https://orcid.org/0000-0002-3020-9564
1000 Label
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  1. Bundesministerium für Gesundheit |
1000 Fördernummer
  1. -
1000 Förderprogramm
  1. grant b (VARIPATH)
1000 Dateien
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  1. 1000 joinedFunding-child
    1000 Förderer Bundesministerium für Gesundheit |
    1000 Förderprogramm grant b (VARIPATH)
    1000 Fördernummer -
1000 Objektart article
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1000 @id frl:6432332.rdf
1000 Erstellt am 2022-03-17T11:05:20.172+0100
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1000 Zuletzt bearbeitet 2022-04-12T13:19:09.663+0200
1000 Objekt bearb. Tue Apr 12 13:18:34 CEST 2022
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1000 Oai Id
  1. oai:frl.publisso.de:frl:6432332 |
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