Liver-expressed Cd302 and Cr1l limit hepatitis C virus cross-species transmission to mice

  1. Brown, Richard ORCID logo
  2. Reinecke, Birthe M. ORCID logo
  3. Sheldon, Julie
  4. Khera, Tanvi ORCID logo
  5. Anggakusuma
  6. Todt, Daniel ORCID logo
  7. Vieyres, Gabrielle ORCID logo
  8. Weller, Romy ORCID logo
  9. Joecks, Sebastian ORCID logo
  10. Zhang, Yudi
  11. Sake, Svenja
  12. Bankwitz, Dorothea ORCID logo
  13. Welsch, Kathrin
  14. Ginkel, Corinne ORCID logo
  15. Engelmann, Michael
  16. Gerold, Gisa ORCID logo
  17. Steinmann, Eike ORCID logo
  18. Yuan, Qinggong
  19. Ott, Michael
  20. Vondran, Florian ORCID logo
  21. Krey, Thomas ORCID logo
  22. Ströh, Luisa ORCID logo
  23. Miskey, Csaba ORCID logo
  24. Ivics, Zoltan ORCID logo
  25. Herder, Vanessa ORCID logo
  26. Baumgärtner, Wolfgang ORCID logo
  27. Lauber, Chris ORCID logo
  28. Seifert, Michael ORCID logo
  29. Tarr, Alexander W.
  30. McClure, Patrick ORCID logo
  31. Randall, Glenn ORCID logo
  32. Baktash, Yasmine ORCID logo
  33. Ploss, Alexander ORCID logo
  34. Dao Thi, Viet Loan ORCID logo
  35. Michailidis, Eleftherios ORCID logo
  36. Saeed, Mohsan ORCID logo
  37. Verhoye, Lieven
  38. Meuleman, Philip ORCID logo
  39. Goedecke, Natascha ORCID logo
  40. Wirth, Dagmar ORCID logo
  41. Rice, Charles M.
  42. Pietschmann, Thomas ORCID logo

Download
Upload.pdf 8,00MB
WeightNameValue
1000 Titel
  • Liver-expressed Cd302 and Cr1l limit hepatitis C virus cross-species transmission to mice
1000 Autor/in
  1. Brown, Richard |
  2. Reinecke, Birthe M. |
  3. Sheldon, Julie |
  4. Khera, Tanvi |
  5. Anggakusuma |
  6. Todt, Daniel |
  7. Vieyres, Gabrielle |
  8. Weller, Romy |
  9. Joecks, Sebastian |
  10. Zhang, Yudi |
  11. Sake, Svenja |
  12. Bankwitz, Dorothea |
  13. Welsch, Kathrin |
  14. Ginkel, Corinne |
  15. Engelmann, Michael |
  16. Gerold, Gisa |
  17. Steinmann, Eike |
  18. Yuan, Qinggong |
  19. Ott, Michael |
  20. Vondran, Florian |
  21. Krey, Thomas |
  22. Ströh, Luisa |
  23. Miskey, Csaba |
  24. Ivics, Zoltan |
  25. Herder, Vanessa |
  26. Baumgärtner, Wolfgang |
  27. Lauber, Chris |
  28. Seifert, Michael |
  29. Tarr, Alexander W. |
  30. McClure, Patrick |
  31. Randall, Glenn |
  32. Baktash, Yasmine |
  33. Ploss, Alexander |
  34. Dao Thi, Viet Loan |
  35. Michailidis, Eleftherios |
  36. Saeed, Mohsan |
  37. Verhoye, Lieven |
  38. Meuleman, Philip |
  39. Goedecke, Natascha |
  40. Wirth, Dagmar |
  41. Rice, Charles M. |
  42. Pietschmann, Thomas |
1000 Erscheinungsjahr 2020
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2020-11-04
1000 Erschienen in
1000 Quellenangabe
  • 6:eabd3233
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2020
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1126/sciadv.abd3233 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673688/ |
1000 Ergänzendes Material
  • https://www.science.org/doi/10.1126/sciadv.abd3233#supplementary-materials |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Hepatitis C virus (HCV) has no animal reservoir, infecting only humans. To investigate species barrier determinants limiting infection of rodents, murine liver complementary DNA library screening was performed, identifying transmembrane proteins Cd302 and Cr1l as potent restrictors of HCV propagation. Combined ectopic expression in human hepatoma cells impeded HCV uptake and cooperatively mediated transcriptional dysregulation of a noncanonical program of immunity genes. Murine hepatocyte expression of both factors was constitutive and not interferon inducible, while differences in liver expression and the ability to restrict HCV were observed between the murine orthologs and their human counterparts. Genetic ablation of endogenous Cd302 expression in human HCV entry factor transgenic mice increased hepatocyte permissiveness for an adapted HCV strain and dysregulated expression of metabolic process and host defense genes. These findings highlight human-mouse differences in liver-intrinsic antiviral immunity and facilitate the development of next-generation murine models for preclinical testing of HCV vaccine candidates.
1000 Sacherschließung
gnd 4262007-7 Hepatitis C
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0002-3292-6671|https://orcid.org/0000-0003-3875-0707|https://frl.publisso.de/adhoc/uri/IFNoZWxkb24sIEp1bGllIA==|https://orcid.org/0000-0002-1287-3197|https://frl.publisso.de/adhoc/uri/QW5nZ2FrdXN1bWE=|https://orcid.org/0000-0002-3564-1014|https://orcid.org/0000-0001-8511-2164|https://orcid.org/0000-0002-6290-457X|https://orcid.org/0000-0003-0248-5374|https://frl.publisso.de/adhoc/uri/IFpoYW5nLCBZdWRpICA=|https://frl.publisso.de/adhoc/uri/ICBTYWtlLCBTdmVuamEgICA=|https://orcid.org/0000-0002-8185-3202|https://frl.publisso.de/adhoc/uri/IFdlbHNjaCwgS2F0aHJpbiAg|https://orcid.org/0000-0002-4876-5681|https://frl.publisso.de/adhoc/uri/RW5nZWxtYW5uLCBNaWNoYWVs|https://orcid.org/0000-0002-1326-5038|https://orcid.org/0000-0002-8771-4262|https://frl.publisso.de/adhoc/uri/IFl1YW4sIFFpbmdnb25nICAg|https://frl.publisso.de/adhoc/uri/IE90dCwgTWljaGFlbCAg|https://orcid.org/0000-0001-8355-5017|https://orcid.org/0000-0002-4548-7241|https://orcid.org/0000-0002-2781-2303|https://orcid.org/0000-0002-7566-9556|https://orcid.org/0000-0002-7803-6658|https://orcid.org/0000-0003-4814-1382|https://orcid.org/0000-0001-8151-5644|https://orcid.org/0000-0002-2265-2953|https://orcid.org/0000-0002-2844-053X|https://frl.publisso.de/adhoc/uri/VGFyciwgQWxleGFuZGVyIFcuICA=|https://orcid.org/0000-0002-1710-1049|https://orcid.org/0000-0001-7417-3615|https://orcid.org/0000-0002-1027-9207|https://orcid.org/0000-0001-9322-7252|https://orcid.org/0000-0003-2293-3592|https://orcid.org/0000-0002-9907-4346|https://orcid.org/0000-0001-8505-7054|https://frl.publisso.de/adhoc/uri/ICAgVmVyaG95ZSwgTGlldmVuICA=|https://orcid.org/0000-0001-6821-234X|https://orcid.org/0000-0002-6578-6601|https://orcid.org/0000-0002-2541-6251|https://frl.publisso.de/adhoc/uri/ICBSaWNlLCBDaGFybGVzIE0uICAg|https://orcid.org/0000-0001-6789-4422
1000 Label
1000 Förderer
  1. National Institute of Allergy and Infectious Diseases |
  2. American Cancer Society |
  3. Burroughs Wellcome Fund |
  4. FWO-Vlaanderen |
  5. Deutsche Forschungsgemeinschaft |
  6. European Research Council |
  7. Deutsches Zentrum für Infektionsforschung |
  8. National Institute of Allergy and Infectious Diseases |
  9. Bundesministerium für Gesundheit |
1000 Fördernummer
  1. R01AI107301-06
  2. RSG-15-048-01-MPC
  3. -
  4. G047417N; VirEOS 30981113
  5. 158989968; 39087428; GE 2145/3-2
  6. ERC-2011-StG_281473
  7. R01AI107301
  8. R01AI107301
  9. 1-2516-FSB-416
1000 Förderprogramm
  1. -
  2. -
  3. Award for Investigators in Pathogenesis program
  4. -
  5. CRC 900; EXC 2155 “RESIST”
  6. VIRAFRONT
  7. Delphi grant
  8. -
  9. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer National Institute of Allergy and Infectious Diseases |
    1000 Förderprogramm -
    1000 Fördernummer R01AI107301-06
  2. 1000 joinedFunding-child
    1000 Förderer American Cancer Society |
    1000 Förderprogramm -
    1000 Fördernummer RSG-15-048-01-MPC
  3. 1000 joinedFunding-child
    1000 Förderer Burroughs Wellcome Fund |
    1000 Förderprogramm Award for Investigators in Pathogenesis program
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer FWO-Vlaanderen |
    1000 Förderprogramm -
    1000 Fördernummer G047417N; VirEOS 30981113
  5. 1000 joinedFunding-child
    1000 Förderer Deutsche Forschungsgemeinschaft |
    1000 Förderprogramm CRC 900; EXC 2155 “RESIST”
    1000 Fördernummer 158989968; 39087428; GE 2145/3-2
  6. 1000 joinedFunding-child
    1000 Förderer European Research Council |
    1000 Förderprogramm VIRAFRONT
    1000 Fördernummer ERC-2011-StG_281473
  7. 1000 joinedFunding-child
    1000 Förderer Deutsches Zentrum für Infektionsforschung |
    1000 Förderprogramm Delphi grant
    1000 Fördernummer R01AI107301
  8. 1000 joinedFunding-child
    1000 Förderer National Institute of Allergy and Infectious Diseases |
    1000 Förderprogramm -
    1000 Fördernummer R01AI107301
  9. 1000 joinedFunding-child
    1000 Förderer Bundesministerium für Gesundheit |
    1000 Förderprogramm -
    1000 Fördernummer 1-2516-FSB-416
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6432392.rdf
1000 Erstellt am 2022-03-21T15:16:20.833+0100
1000 Erstellt von 323
1000 beschreibt frl:6432392
1000 Bearbeitet von 317
1000 Zuletzt bearbeitet 2022-04-20T11:59:33.063+0200
1000 Objekt bearb. Wed Apr 20 11:59:32 CEST 2022
1000 Vgl. frl:6432392
1000 Oai Id
  1. oai:frl.publisso.de:frl:6432392 |
1000 Sichtbarkeit Metadaten public
1000 Sichtbarkeit Daten public
1000 Gegenstand von

View source