Establishment of Transfusion-Relevant Bacteria Reference Strains for Red Blood Cells

  1. Prax, Marcel ORCID logo
  2. Spindler-Raffel, Eva
  3. McDonald, Carl ORCID logo
  4. Bearne, Jennifer
  5. Satake, Masahiro
  6. Kozakai, Moe ORCID logo
  7. Rojo, Julieta
  8. Hanschmann, Kay-Martin Ove ORCID logo
  9. Lambrecht, Bernd
  10. Grundmann, Udo
  11. O'Flaherty, Niamh ORCID logo
  12. Klimek-Zdeb, Agata ORCID logo
  13. Bekeredjian-Ding, Isabelle ORCID logo
  14. Gathof, Birgit ORCID logo
  15. Störmer, Melanie
  16. Süßner, Susanne
  17. Renke, Claudia
  18. LEE, Cheukkwong ORCID logo
  19. Knabbe, Cornelius
  20. Vollmer, Tanja ORCID logo
  21. Keil, Shawn ORCID logo
  22. Shipps, Marley E.
  23. Wagner, Stephen J.
  24. Jentsch, Ute ORCID logo
  25. Mpumlwana, Xoliswa
  26. Cloutier, Marc ORCID logo
  27. Bringmann, Peter
  28. Lu, Thea
  29. Ramirez-Arcos, Sandra ORCID logo
  30. Kou, Yuntong
  31. Krut, Oleg ORCID logo
  32. ISBT Transfusion-Transmitted Infectious Diseases Working Party, Subgroup on Bacteria

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WeightNameValue
1000 Titel
  • Establishment of Transfusion-Relevant Bacteria Reference Strains for Red Blood Cells
1000 Autor/in
  1. Prax, Marcel |
  2. Spindler-Raffel, Eva |
  3. McDonald, Carl |
  4. Bearne, Jennifer |
  5. Satake, Masahiro |
  6. Kozakai, Moe |
  7. Rojo, Julieta |
  8. Hanschmann, Kay-Martin Ove |
  9. Lambrecht, Bernd |
  10. Grundmann, Udo |
  11. O'Flaherty, Niamh |
  12. Klimek-Zdeb, Agata |
  13. Bekeredjian-Ding, Isabelle |
  14. Gathof, Birgit |
  15. Störmer, Melanie |
  16. Süßner, Susanne |
  17. Renke, Claudia |
  18. LEE, Cheukkwong |
  19. Knabbe, Cornelius |
  20. Vollmer, Tanja |
  21. Keil, Shawn |
  22. Shipps, Marley E. |
  23. Wagner, Stephen J. |
  24. Jentsch, Ute |
  25. Mpumlwana, Xoliswa |
  26. Cloutier, Marc |
  27. Bringmann, Peter |
  28. Lu, Thea |
  29. Ramirez-Arcos, Sandra |
  30. Kou, Yuntong |
  31. Krut, Oleg |
1000 Mitwirkende/r
  1. ISBT Transfusion-Transmitted Infectious Diseases Working Party, Subgroup on Bacteria |
1000 Erscheinungsjahr 2020
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2020-12-20
1000 Erschienen in
1000 Quellenangabe
  • 116(6):692-701
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2020
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1111/vox.13057 |
1000 Ergänzendes Material
  • https://onlinelibrary.wiley.com/doi/10.1111/vox.13057#support-information-section |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND AND OBJECTIVES: Red blood cell concentrates (RBCC) are susceptible to bacterial contamination despite cold storage. A reliable evaluation of strategies to minimize the risk of RBCC-associated bacterial transmission requires the use of suitable reference bacteria. Already existing Transfusion-Relevant Bacteria Reference Strains (TRBRS) for platelet concentrates fail to grow in RBCC. Consequently, the ISBT TTID, Working Party, Bacterial Subgroup, conducted an international study on TRBRS for RBCC. MATERIALS AND METHODS: Six bacterial strains (Listeria monocytogenes PEI-A-199, Serratia liquefaciens PEI-A-184, Serratia marcescens PEI-B-P-56, Pseudomonas fluorescens PEI-B-P-77, Yersinia enterocolitica PEI-A-105, Yersinia enterocolitica PEI-A-176) were distributed to 15 laboratories worldwide for enumeration, identification, and determination of growth kinetics in RBCC at days 7, 14, 21, 28, 35 and 42 of storage after low-count spiking (10–25 CFU/RBCC). RESULTS: Bacterial proliferation in RBCC was obtained for most strains, except for S. marcescens, which grew only at 4 of 15 laboratories. S. liquefaciens, S. marcescens, P. fluorescens and the two Y. enterocolitica strains reached the stationary phase between days 14 and 21 of RBCC storage with a bacterial concentration of approximately 109 CFU/ml. L. monocytogenes displayed slower growth kinetics reaching 106–107 CFU/ml after 42 days. CONCLUSION: The results illustrate the importance of conducting comprehensive studies to establish well-characterized reference strains, which can be a tool to assess strategies and methods used to ameliorate blood safety. The WHO Expert Committee on Biological Standardization adopted the five successful strains as official RBCC reference strains. Our study also highlights the relevance of visual inspection to interdict contaminated RBC units.
1000 Sacherschließung
lokal contamination
lokal sepsis
lokal reference material
lokal blood safety
gnd 4007293-9 Bluttransfusion
lokal bacteria
lokal red blood cells
lokal validation
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1000 Liste der Beteiligten
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