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1000 Titel
  • Zika virus infection studies with CD34+ hematopoietic and megakaryocyte-erythroid progenitors, red blood cells and platelets
1000 Autor/in
  1. Roth, Hanna |
  2. Schneider, Lucas |
  3. Eberle, Regina |
  4. Lausen, Jörn |
  5. Modlich, Ute |
  6. Bluemel, Johannes |
  7. Baylis, Sally |
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2020-02-22
1000 Erschienen in
1000 Quellenangabe
  • 60(3):561-574
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2020
1000 Lizenz
1000 Verlagsversion
  • |
1000 Ergänzendes Material
  • |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • BACKGROUND: To date, several cases of transfusion-transmitted ZIKV infections have been confirmed. Multiple studies detected prolonged occurrence of ZIKV viral RNA in whole blood as compared to plasma samples indicating potential ZIKV interaction with hematopoietic cells. Also, infection of cells from the granulocyte/macrophage lineage has been demonstrated. Patients may develop severe thrombocytopenia, microcytic anemia, and a fatal course of disease occurred in a patient with sickle cell anemia suggesting additional interference of ZIKV with erythroid and megakaryocytic cells. Therefore, we analyzed whether ZIKV propagates in or compartmentalizes with hematopoietic progenitor, erythroid, and megakaryocytic cells. METHODS: ZIKV RNA replication, protein translation and infectious particle formation in hematopoietic cell lines as well as primary CD34+ HSPCs and ex vivo differentiated erythroid and megakaryocytic cells was monitored using qRT-PCR, FACS, immunofluorescence analysis and infectivity assays. Distribution of ZIKV RNA and infectious particles in spiked red blood cell (RBC) units or platelet concentrates (PCs) was evaluated. RESULTS: While subsets of K562 and KU812Ep6EPO cells supported ZIKV propagation, primary CD34+ HSPCs, MEP cells, RBCs, and platelets were non-permissive for ZIKV infection. In spiking studies, ZIKV RNA was detectable for 7 days in all fractions of RBC units and PCs, however, ZIKV infectious particles were not associated with erythrocytes or platelets. CONCLUSION: Viral particles from plasma or contaminating leukocytes, rather than purified CD34+ HSPCs or the cellular component of RBC units or PCs, present the greatest risk for transfusion-transmitted ZIKV infections.
1000 Sacherschließung
gnd 4007293-9 Bluttransfusion
lokal Zika-Virus
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
1000 Label
1000 Förderer
  1. Bundesministerium für Gesundheit |
1000 Fördernummer
  1. -
1000 Förderprogramm
  1. Sicherheit von Blut(produkten) und Geweben hinsichtlich der Abwesenheit von Zikaviren
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Bundesministerium für Gesundheit |
    1000 Förderprogramm Sicherheit von Blut(produkten) und Geweben hinsichtlich der Abwesenheit von Zikaviren
    1000 Fördernummer -
1000 Objektart article
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1000 @id frl:6432776.rdf
1000 Erstellt am 2022-04-01T10:27:59.998+0200
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