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1000 Titel
  • Signalling pathways and mechanistic cues highlighted by transcriptomic analysis of primordial, primary, and secondary ovarian follicles in domestic cat
1000 Autor/in
  1. Kehoe, Shauna |
  2. Jewgenow, Katarina |
  3. Johnston, Paul R. |
  4. Mbedi, Susan |
  5. Braun, Beate C. |
1000 Erscheinungsjahr 2021
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-01-29
1000 Erschienen in
1000 Quellenangabe
  • 11(1):2683
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1038/s41598-021-82051-4 |
1000 Ergänzendes Material
  • https://www.nature.com/articles/s41598-021-82051-4#Sec16 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • In vitro growth (IVG) of dormant primordial ovarian follicles aims to produce mature competent oocytes for assisted reproduction. Success is dependent on optimal in vitro conditions complemented with an understanding of oocyte and ovarian follicle development in vivo. Complete IVG has not been achieved in any other mammalian species besides mice. Furthermore, ovarian folliculogenesis remains sparsely understood overall. Here, gene expression patterns were characterised by RNA-sequencing in primordial (PrF), primary (PF), and secondary (SF) ovarian follicles from Felis catus (domestic cat) ovaries. Two major transitions were investigated: PrF-PF and PF-SF. Transcriptional analysis revealed a higher proportion in gene expression changes during the PrF-PF transition. Key influencing factors during this transition included the interaction between the extracellular matrix (ECM) and matrix metalloproteinase (MMPs) along with nuclear components such as, histone HIST1H1T (H1.6). Conserved signalling factors and expression patterns previously described during mammalian ovarian folliculogenesis were observed. Species-specific features during domestic cat ovarian folliculogenesis were also found. The signalling pathway terms “PI3K-Akt”, “transforming growth factor-β receptor”, “ErbB”, and “HIF-1” from the functional annotation analysis were studied. Some results highlighted mechanistic cues potentially involved in PrF development in the domestic cat. Overall, this study provides an insight into regulatory factors and pathways during preantral ovarian folliculogenesis in domestic cat.
1000 Sacherschließung
lokal Gene expression
lokal Gene expression analysis
lokal Bioinformatics
lokal Sequencing
lokal Cell biology
lokal RNA
lokal Transcriptomics
lokal Cell signalling
lokal Development
lokal Gene regulation
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  1. https://frl.publisso.de/adhoc/uri/S2Vob2UsIFNoYXVuYQ==|https://frl.publisso.de/adhoc/uri/SmV3Z2Vub3csIEthdGFyaW5h|https://frl.publisso.de/adhoc/uri/Sm9obnN0b24sIFBhdWwgUi4=|https://frl.publisso.de/adhoc/uri/TWJlZGksIFN1c2Fu|https://frl.publisso.de/adhoc/uri/QnJhdW4sIEJlYXRlIEMu
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