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1000 Titel
  • Re-Discovery of Giardiavirus: Genomic and Functional Analysis of Viruses from Giardia duodenalis Isolates
1000 Autor/in
  1. Marucci, Gianluca |
  2. Zullino, Ilaria |
  3. Bertuccini, Lucia |
  4. Camerini, Serena |
  5. Cecchetti, Serena |
  6. Pietrantoni, Agostina |
  7. Casella, Marialuisa |
  8. Vatta, Paolo |
  9. Greenwood, Alex |
  10. FIORILLO, Annarita |
  11. Lalle, Marco |
1000 Erscheinungsjahr 2021
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-06-08
1000 Erschienen in
1000 Quellenangabe
  • 9(6):654
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.3390/biomedicines9060654 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8230311/ |
1000 Ergänzendes Material
  • https://www.mdpi.com/2227-9059/9/6/654#supplementary |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Giardiasis, caused by the protozoan parasite Giardia duodenalis, is an intestinal diarrheal disease affecting almost one billion people worldwide. A small endosymbiotic dsRNA viruses, G. lamblia virus (GLV), genus Giardiavirus, family Totiviridae, might inhabit human and animal isolates of G. duodenalis. Three GLV genomes have been sequenced so far, and only one was intensively studied; moreover, a positive correlation between GLV and parasite virulence is yet to be proved. To understand the biological significance of GLV infection in Giardia, the characterization of several GLV strains from naturally infected G. duodenalis isolates is necessary. Here we report high-throughput sequencing of four GLVs strains, from Giardia isolates of human and animal origin. We also report on a new, unclassified viral sequence (designed GdRV-2), unrelated to Giardiavirus, encoding and expressing for a single large protein with an RdRp domain homologous to Totiviridae and Botybirnaviridae. The result of our sequencing and proteomic analyses challenge the current knowledge on GLV and strongly suggest that viral capsid protein translation unusually starts with a proline and that translation of the RNA-dependent RNA polymerase (RdRp) occurs via a +1/−2 ribosomal frameshift mechanism. Nucleotide polymorphism, confirmed by mass-spectrometry analysis, was also observed among and between GLV strains. Phylogenetic analysis indicated the occurrence of at least two GLV subtypes which display different phenotypes and transmissibility in experimental infections of a GLV naïve Giardia isolate.
1000 Sacherschließung
lokal genomics
lokal Giardiavirus
lokal virion
lokal RNA viruses
lokal proteomics
lokal Giardia duodenalis
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/uri/TWFydWNjaSwgR2lhbmx1Y2E=|https://frl.publisso.de/adhoc/uri/WnVsbGlubywgSWxhcmlh|https://orcid.org/0000-0002-4278-2376|https://frl.publisso.de/adhoc/uri/Q2FtZXJpbmksIFNlcmVuYQ==|https://frl.publisso.de/adhoc/uri/Q2VjY2hldHRpLCBTZXJlbmE=|https://frl.publisso.de/adhoc/uri/UGlldHJhbnRvbmksIEFnb3N0aW5h|https://frl.publisso.de/adhoc/uri/Q2FzZWxsYSwgTWFyaWFsdWlzYQ==|https://frl.publisso.de/adhoc/uri/VmF0dGEsIFBhb2xv|https://orcid.org/0000-0002-8249-1565|https://orcid.org/0000-0002-8966-8554|https://orcid.org/0000-0001-5168-2150
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1000 Erstellt am 2022-07-27T12:41:18.360+0200
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1000 Zuletzt bearbeitet 2022-08-05T16:09:16.573+0200
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