Mesenchymal stromal cells mitigate liver damage after extended resection in the pig by modulating thrombospondin-1/TGF-β

  1. Nickel, Sandra
  2. Vlaic, Sebastian
  3. Christ, Madlen
  4. Schubert, Kristin ORCID logo
  5. Henschler, Reinhard
  6. Tautenhahn, Franziska
  7. Burger, Caroline
  8. Kühne, Hagen
  9. Erler, Silvio
  10. Roth, Andreas ORCID logo
  11. Wild, Christiane ORCID logo
  12. Brach, Janine
  13. Hammad, Seddik
  14. Gittel, Claudia ORCID logo
  15. Baunack, Manja
  16. Lange, Undine
  17. Broschewitz, Johannes ORCID logo
  18. Stock, Peggy ORCID logo
  19. Metelmann, Isabella Bettine ORCID logo
  20. Bartels, Michael
  21. Pietsch, Uta-Carolin
  22. Krämer, Sebastian ORCID logo
  23. Eichfeld, Uwe
  24. von Bergen, Martin
  25. Dooley, Steven
  26. Tautenhahn, Hans-Michael
  27. Christ, Bruno ORCID logo

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WeightNameValue
1000 Titel
  • Mesenchymal stromal cells mitigate liver damage after extended resection in the pig by modulating thrombospondin-1/TGF-β
1000 Autor/in
  1. Nickel, Sandra |
  2. Vlaic, Sebastian |
  3. Christ, Madlen |
  4. Schubert, Kristin |
  5. Henschler, Reinhard |
  6. Tautenhahn, Franziska |
  7. Burger, Caroline |
  8. Kühne, Hagen |
  9. Erler, Silvio |
  10. Roth, Andreas |
  11. Wild, Christiane |
  12. Brach, Janine |
  13. Hammad, Seddik |
  14. Gittel, Claudia |
  15. Baunack, Manja |
  16. Lange, Undine |
  17. Broschewitz, Johannes |
  18. Stock, Peggy |
  19. Metelmann, Isabella Bettine |
  20. Bartels, Michael |
  21. Pietsch, Uta-Carolin |
  22. Krämer, Sebastian |
  23. Eichfeld, Uwe |
  24. von Bergen, Martin |
  25. Dooley, Steven |
  26. Tautenhahn, Hans-Michael |
  27. Christ, Bruno |
1000 Erscheinungsjahr 2021
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-12-03
1000 Erschienen in
1000 Quellenangabe
  • 6(1):84
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1038/s41536-021-00194-4 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8642541/ |
1000 Publikationsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Post-surgery liver failure is a serious complication for patients after extended partial hepatectomies (ePHx). Previously, we demonstrated in the pig model that transplantation of mesenchymal stromal cells (MSC) improved circulatory maintenance and supported multi-organ functions after 70% liver resection. Mechanisms behind the beneficial MSC effects remained unknown. Here we performed 70% liver resection in pigs with and without MSC treatment, and animals were monitored for 24 h post surgery. Gene expression profiles were determined in the lung and liver. Bioinformatics analysis predicted organ-independent MSC targets, importantly a role for thrombospondin-1 linked to transforming growth factor-β (TGF-β) and downstream signaling towards providing epithelial plasticity and epithelial-mesenchymal transition (EMT). This prediction was supported histologically and mechanistically, the latter with primary hepatocyte cell cultures. MSC attenuated the surgery-induced increase of tissue damage, of thrombospondin-1 and TGF-β, as well as of epithelial plasticity in both the liver and lung. This suggests that MSC ameliorated surgery-induced hepatocellular stress and EMT, thus supporting epithelial integrity and facilitating regeneration. MSC-derived soluble factor(s) did not directly interfere with intracellular TGF-β signaling, but inhibited thrombospondin-1 secretion from thrombocytes and non-parenchymal liver cells, therewith obviously reducing the availability of active TGF-β.
1000 Sacherschließung
lokal Article
lokal Regenerative medicine
lokal Mesenchymal stem cells
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/uri/Tmlja2VsLCBTYW5kcmE=|https://frl.publisso.de/adhoc/uri/VmxhaWMsIFNlYmFzdGlhbg==|https://frl.publisso.de/adhoc/uri/Q2hyaXN0LCBNYWRsZW4=|https://orcid.org/0000-0003-4365-084X|https://frl.publisso.de/adhoc/uri/SGVuc2NobGVyLCBSZWluaGFyZA==|https://frl.publisso.de/adhoc/uri/VGF1dGVuaGFobiwgRnJhbnppc2th|https://frl.publisso.de/adhoc/uri/QnVyZ2VyLCBDYXJvbGluZQ==|https://frl.publisso.de/adhoc/uri/S8O8aG5lLCBIYWdlbg==|https://frl.publisso.de/adhoc/uri/RXJsZXIsIFNpbHZpbw==|https://orcid.org/0000-0002-1145-955X|https://orcid.org/0000-0001-9435-6769|https://frl.publisso.de/adhoc/uri/QnJhY2gsIEphbmluZQ==|https://frl.publisso.de/adhoc/uri/SGFtbWFkLCBTZWRkaWs=|https://orcid.org/0000-0003-1447-6299|https://frl.publisso.de/adhoc/uri/QmF1bmFjaywgTWFuamE=|https://frl.publisso.de/adhoc/uri/TGFuZ2UsIFVuZGluZQ==|https://orcid.org/0000-0002-3390-735X|https://orcid.org/0000-0002-9208-8411|https://orcid.org/0000-0003-1963-8189|https://frl.publisso.de/adhoc/uri/QmFydGVscywgTWljaGFlbA==|https://frl.publisso.de/adhoc/uri/UGlldHNjaCwgVXRhLUNhcm9saW4=|https://orcid.org/0000-0003-4867-8884|https://frl.publisso.de/adhoc/uri/RWljaGZlbGQsIFV3ZQ==|https://frl.publisso.de/adhoc/uri/dm9uIEJlcmdlbiwgTWFydGlu|https://frl.publisso.de/adhoc/uri/RG9vbGV5LCBTdGV2ZW4=|https://frl.publisso.de/adhoc/uri/VGF1dGVuaGFobiwgSGFucy1NaWNoYWVs|https://orcid.org/0000-0001-8213-9548
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  • DeepGreen-ID: 88821b4e132244f199e86b46ec6aac59 ; metadata provieded by: DeepGreen (https://www.oa-deepgreen.de/api/v1/), LIVIVO search scope life sciences (http://z3950.zbmed.de:6210/livivo), Crossref Unified Resource API (https://api.crossref.org/swagger-ui/index.html), to.science.api (https://frl.publisso.de/), ZDB JSON-API (beta) (https://zeitschriftendatenbank.de/api/), lobid - Dateninfrastruktur für Bibliotheken (https://lobid.org/resources/search)
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1000 Erstellt am 2023-04-27T09:53:59.426+0200
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