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1000 Titel
  • Is the bioactivity of induced membranes time dependent?
1000 Autor/in
  1. Gessmann, Jan |
  2. Rosteius, Thomas |
  3. Baecker, Hinnerk |
  4. Sivalingam, Kavitha |
  5. Peter, Elvira |
  6. Schildhauer, Thomas Armin |
  7. Köller, Manfred |
1000 Erscheinungsjahr 2021
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-12-06
1000 Erschienen in
1000 Quellenangabe
  • 48(4):3051-3061
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1007/s00068-021-01844-4 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360131/ |
1000 Publikationsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • Purpose!#!The induced membrane technique (IMT) is a two-stage surgical procedure for reconstruction of bone defects. Bone grafting (second stage of IMT) is recommend after 4-8 weeks assuming the highest bioactivity of IMs. However, larger studies concerning the biology and maturation of IMs and a potential time dependency of the bioactivity are missing. Therefore, aim of this study was the time-dependent structural and cellular characterization of cement spacer IMs concomitantly to an analysis of membrane bioactivity.!##!Methods!#!IMs from 60 patients (35-82 years) were obtained at different maturation stages (1-16 weeks). IMs were studied by histology and co-culture with mesenchymal stem cells (MSC). IM lysates were analyzed by ELISA and protein microarray.!##!Results!#!Increasing vascularization and fibrosis were found in membranes older than 4 and 7 weeks, respectively. MSC grew out from all membranes and all membranes enhanced proliferation of cultured MSC. Osteocalcin and osteopontin (in membrane lysates or induced in MSC by membrane tissue) were found over all time points without significant differences. In contrast to alkaline phosphatase activity, increasing levels of osteoprotegerin were found in membranes.!##!Conclusion!#!The histological structure of IMs changes during growth and maturation, however, biologically active MSC and factors related to osteogenesis are found over all time points with minor changes. Thus, membranes older than 8 weeks exert regenerative capacities comparable to the younger ones. The postulated narrow time frame of 4-8 weeks until bone grafting can be questioned and surgeons may choose timing for the second operation more independently and based on other clinical factors.
1000 Sacherschließung
lokal Original Article
lokal Osteocalcin [MeSH]
lokal Osteogenesis [MeSH]
lokal Bioactivity
lokal Induced membrane
lokal Bone defect
lokal Humans [MeSH]
lokal Masquelet
lokal Coculture Techniques [MeSH]
lokal Membranes [MeSH]
lokal Mesenchymal stem cells
lokal Mesenchymal Stem Cells/metabolism [MeSH]
1000 Liste der Beteiligten
  1. https://frl.publisso.de/adhoc/uri/R2Vzc21hbm4sIEphbg==|https://orcid.org/0000-0002-3382-0510|https://frl.publisso.de/adhoc/uri/QmFlY2tlciwgSGlubmVyaw==|https://frl.publisso.de/adhoc/uri/U2l2YWxpbmdhbSwgS2F2aXRoYQ==|https://frl.publisso.de/adhoc/uri/UGV0ZXIsIEVsdmlyYQ==|https://frl.publisso.de/adhoc/uri/U2NoaWxkaGF1ZXIsIFRob21hcyBBcm1pbg==|https://frl.publisso.de/adhoc/uri/S8O2bGxlciwgTWFuZnJlZA==
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1000 Erstellt am 2023-05-03T18:27:38.084+0200
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1000 Zuletzt bearbeitet 2023-10-20T22:42:31.403+0200
1000 Objekt bearb. Fri Oct 20 22:42:31 CEST 2023
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