Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction

  1. Zywitza, Vera
  2. Rusha, Ejona
  3. Shaposhnikov, Dmitry
  4. Ruiz-Orera, Jorge
  5. Telugu, Narasimha
  6. Rishko, Valentyna
  7. Hayashi, Masafumi
  8. Michel, Geert
  9. Wittler, Lars
  10. Stejskal, Jan
  11. Holtze, Susanne
  12. Göritz, Frank
  13. Hermes, Robert
  14. Wang, Jichang
  15. Izsvák, Zsuzsanna
  16. Colleoni, Silvia
  17. Lazzari, Giovanna
  18. Galli, Cesare
  19. Hildebrandt, Thomas B.
  20. Hayashi, Katsuhiko
  21. Diecke, Sebastian
  22. Drukker, Micha

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WeightNameValue
1000 Titel
  • Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction
1000 Autor/in
  1. Zywitza, Vera |
  2. Rusha, Ejona |
  3. Shaposhnikov, Dmitry |
  4. Ruiz-Orera, Jorge |
  5. Telugu, Narasimha |
  6. Rishko, Valentyna |
  7. Hayashi, Masafumi |
  8. Michel, Geert |
  9. Wittler, Lars |
  10. Stejskal, Jan |
  11. Holtze, Susanne |
  12. Göritz, Frank |
  13. Hermes, Robert |
  14. Wang, Jichang |
  15. Izsvák, Zsuzsanna |
  16. Colleoni, Silvia |
  17. Lazzari, Giovanna |
  18. Galli, Cesare |
  19. Hildebrandt, Thomas B. |
  20. Hayashi, Katsuhiko |
  21. Diecke, Sebastian |
  22. Drukker, Micha |
1000 Erscheinungsjahr 2022
1000 LeibnizOpen
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2022-03-08
1000 Erschienen in
1000 Quellenangabe
  • 12(1):3100
1000 FRL-Sammlung
1000 Copyrightjahr
  • 2022
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1038/s41598-022-07059-w |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8904600/ |
1000 Ergänzendes Material
  • https://www.nature.com/articles/s41598-022-07059-w#Sec34 |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • The northern white rhinoceros (NWR) is probably the earth's most endangered mammal. To rescue the functionally extinct species, we aim to employ induced pluripotent stem cells (iPSCs) to generate gametes and subsequently embryos in vitro. To elucidate the regulation of pluripotency and differentiation of NWR PSCs, we generated iPSCs from a deceased NWR female using episomal reprogramming, and observed surprising similarities to human PSCs. NWR iPSCs exhibit a broad differentiation potency into the three germ layers and trophoblast, and acquire a naïve-like state of pluripotency, which is pivotal to differentiate PSCs into primordial germ cells (PGCs). Naïve culturing conditions induced a similar expression profile of pluripotency related genes in NWR iPSCs and human ESCs. Furthermore, naïve-like NWR iPSCs displayed increased expression of naïve and PGC marker genes, and a higher integration propensity into developing mouse embryos. As the conversion process was aided by ectopic BCL2 expression, and we observed integration of reprogramming factors, the NWR iPSCs presented here are unsuitable for gamete production. However, the gained insights into the developmental potential of both primed and naïve-like NWR iPSCs are fundamental for in future PGC-specification in order to rescue the species from extinction using cryopreserved somatic cells.
1000 Sacherschließung
lokal Induced Pluripotent Stem Cells [MeSH]
lokal Female [MeSH]
lokal Multidisciplinary
lokal Biological techniques
lokal Stem cells
lokal Developmental biology
lokal Cell Differentiation/genetics [MeSH]
lokal Animals [MeSH]
lokal Perissodactyla/genetics [MeSH]
lokal Mice [MeSH]
lokal Germ Cells/metabolism [MeSH]
lokal Germ Layers [MeSH]
lokal Cell biology
1000 Fächerklassifikation (DDC)
1000 Liste der Beteiligten
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1000 Label
1000 Förderer
  1. Projekt DEAL |
  2. Bundesministerium für Bildung und Forschung |
  3. Japanese KAKENHI Grants-in-Aid from MEXT |
  4. Rewind Rhino Extinction |
1000 Fördernummer
  1. -
  2. 01LC1902B
  3. -
  4. -
1000 Förderprogramm
  1. Open Access funding
  2. -
  3. -
  4. -
1000 Dateien
1000 Förderung
  1. 1000 joinedFunding-child
    1000 Förderer Projekt DEAL |
    1000 Förderprogramm Open Access funding
    1000 Fördernummer -
  2. 1000 joinedFunding-child
    1000 Förderer Bundesministerium für Bildung und Forschung |
    1000 Förderprogramm -
    1000 Fördernummer 01LC1902B
  3. 1000 joinedFunding-child
    1000 Förderer Japanese KAKENHI Grants-in-Aid from MEXT |
    1000 Förderprogramm -
    1000 Fördernummer -
  4. 1000 joinedFunding-child
    1000 Förderer Rewind Rhino Extinction |
    1000 Förderprogramm -
    1000 Fördernummer -
1000 Objektart article
1000 Beschrieben durch
1000 @id frl:6449450.rdf
1000 Erstellt am 2023-05-05T09:10:47.668+0200
1000 Erstellt von 336
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1000 Bearbeitet von 317
1000 Zuletzt bearbeitet Mon May 22 12:02:24 CEST 2023
1000 Objekt bearb. Mon May 22 12:02:10 CEST 2023
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