Status quo of ALK testing in lung cancer: results of an EQA scheme based on in-situ hybridization, immunohistochemistry, and RNA/DNA sequencing

  1. Jurmeister, Philipp ORCID logo
  2. Vollbrecht, Claudia
  3. Jöhrens, Korinna
  4. Aust, Daniela
  5. Behnke, Anke
  6. Stenzinger, Albrecht
  7. Penzel, Roland
  8. Endris, Volker
  9. Schirmacher, Peter
  10. Fisseler-Eckhoff, Annette
  11. Neumann, Jens
  12. Kirchner, Thomas
  13. Büttner, Reinhard
  14. Merkelbach-Bruse, Sabine
  15. Kreipe, Hans
  16. Jonigk, Danny
  17. Jochum, Wolfram
  18. Rodriguez, Regulo
  19. Dietel, Manfred
  20. Horst, David
  21. Hummel, Michael
  22. von Laffert, Maximilian

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1000 Titel
  • Status quo of ALK testing in lung cancer: results of an EQA scheme based on in-situ hybridization, immunohistochemistry, and RNA/DNA sequencing
1000 Autor/in
  1. Jurmeister, Philipp |
  2. Vollbrecht, Claudia |
  3. Jöhrens, Korinna |
  4. Aust, Daniela |
  5. Behnke, Anke |
  6. Stenzinger, Albrecht |
  7. Penzel, Roland |
  8. Endris, Volker |
  9. Schirmacher, Peter |
  10. Fisseler-Eckhoff, Annette |
  11. Neumann, Jens |
  12. Kirchner, Thomas |
  13. Büttner, Reinhard |
  14. Merkelbach-Bruse, Sabine |
  15. Kreipe, Hans |
  16. Jonigk, Danny |
  17. Jochum, Wolfram |
  18. Rodriguez, Regulo |
  19. Dietel, Manfred |
  20. Horst, David |
  21. Hummel, Michael |
  22. von Laffert, Maximilian |
1000 Erscheinungsjahr 2021
1000 Publikationstyp
  1. Artikel |
1000 Online veröffentlicht
  • 2021-06-25
1000 Erschienen in
1000 Quellenangabe
  • 479(2):247-255
1000 Copyrightjahr
  • 2021
1000 Lizenz
1000 Verlagsversion
  • https://doi.org/10.1007/s00428-021-03106-5 |
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364534/ |
1000 Publikationsstatus
1000 Begutachtungsstatus
1000 Sprache der Publikation
1000 Abstract/Summary
  • With this external quality assessment (EQA) scheme, we aim to investigate the diagnostic performance of the currently available methods for the detection of ALK alterations in non-small cell lung cancer on a national scale, namely, in situ hybridization (ISH), immunohistochemistry (IHC), and RNA/DNA sequencing (NGS). The EQA scheme cohort consisted of ten specimens, including four ALK positive and six ALK negative samples, which were thoroughly pretested using IHC, ISH, and RNA/DNA NGS. Unstained tumor sections were provided to the 57 participants, and the results were retrieved via an online questionnaire. ISH was used by 29, IHC by 38, and RNA/DNA sequencing by 19 participants. Twenty-eight institutions (97%) passed the ring trial using ISH, 33 (87%) by using IHC, and 18 (95%) by using NGS. The highest sensitivity and interrater agreement (Fleiss ' kappa) was observed for RNA/DNA sequencing (99%, 0.975), followed by ISH (94%, 0.898) and IHC (92%, 0.888). However, the proportion of samples that were not evaluable due to bad tissue quality was also higher for RNA/DNA sequencing (4%) compared with ISH (0.7%) and IHC (0.5%). While all three methods produced reliable results between the different institutions, the highest sensitivity and concordance were observed for RNA/DNA sequencing. These findings encourage the broad implementation of this method in routine diagnostic, although the application might be limited by technical capacity, economical restrictions, and tissue quality of formalin-fixed samples.
1000 Sacherschließung
lokal Anaplastic lymphoma kinase
lokal Observer Variation [MeSH]
lokal Carcinoma, Non-Small-Cell Lung/genetics [MeSH]
lokal Lung Neoplasms/genetics [MeSH]
lokal Original Article
lokal In Situ Hybridization [MeSH]
lokal Lung Neoplasms/enzymology [MeSH]
lokal Next-generation sequencing
lokal Sequence Analysis, DNA [MeSH]
lokal External quality assessment
lokal Carcinoma, Non-Small-Cell Lung/pathology [MeSH]
lokal Laboratory Proficiency Testing [MeSH]
lokal Translocation, Genetic [MeSH]
lokal Humans [MeSH]
lokal Sequence Analysis, RNA [MeSH]
lokal Predictive Value of Tests [MeSH]
lokal Immunohistochemistry [MeSH]
lokal Non-small cell lung cancer
lokal Reproducibility of Results [MeSH]
lokal Biomarkers, Tumor/genetics [MeSH]
lokal Germany [MeSH]
lokal Lung Neoplasms/pathology [MeSH]
lokal Carcinoma, Non-Small-Cell Lung/enzymology [MeSH]
lokal Anaplastic Lymphoma Kinase/genetics [MeSH]
lokal High-Throughput Nucleotide Sequencing [MeSH]
lokal Round robin
1000 Liste der Beteiligten
  1. https://orcid.org/0000-0001-7745-3794|https://frl.publisso.de/adhoc/uri/Vm9sbGJyZWNodCwgQ2xhdWRpYQ==|https://frl.publisso.de/adhoc/uri/SsO2aHJlbnMsIEtvcmlubmE=|https://frl.publisso.de/adhoc/uri/QXVzdCwgRGFuaWVsYQ==|https://frl.publisso.de/adhoc/uri/QmVobmtlLCBBbmtl|https://frl.publisso.de/adhoc/uri/U3RlbnppbmdlciwgQWxicmVjaHQ=|https://frl.publisso.de/adhoc/uri/UGVuemVsLCBSb2xhbmQ=|https://frl.publisso.de/adhoc/uri/RW5kcmlzLCBWb2xrZXI=|https://frl.publisso.de/adhoc/uri/U2NoaXJtYWNoZXIsIFBldGVy|https://frl.publisso.de/adhoc/uri/Rmlzc2VsZXItRWNraG9mZiwgQW5uZXR0ZQ==|https://frl.publisso.de/adhoc/uri/TmV1bWFubiwgSmVucw==|https://frl.publisso.de/adhoc/uri/S2lyY2huZXIsIFRob21hcw==|https://frl.publisso.de/adhoc/uri/QsO8dHRuZXIsIFJlaW5oYXJk|https://frl.publisso.de/adhoc/uri/TWVya2VsYmFjaC1CcnVzZSwgU2FiaW5l|https://frl.publisso.de/adhoc/uri/S3JlaXBlLCBIYW5z|https://frl.publisso.de/adhoc/uri/Sm9uaWdrLCBEYW5ueQ==|https://frl.publisso.de/adhoc/uri/Sm9jaHVtLCBXb2xmcmFt|https://frl.publisso.de/adhoc/uri/Um9kcmlndWV6LCBSZWd1bG8=|https://frl.publisso.de/adhoc/uri/RGlldGVsLCBNYW5mcmVk|https://frl.publisso.de/adhoc/uri/SG9yc3QsIERhdmlk|https://frl.publisso.de/adhoc/uri/SHVtbWVsLCBNaWNoYWVs|https://frl.publisso.de/adhoc/uri/dm9uIExhZmZlcnQsIE1heGltaWxpYW4=
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1000 Erstellt am 2023-05-09T11:01:40.755+0200
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